Betaine attenuates the expression of vasoactive mediators and histological alterations associated with ovarian hyperstimulation syndrome in rats

Articles in Press

Document Type : Original Article

Authors

Shahid Chamran University of Ahvaz, Ahvaz, Iran

10.22074/ijfs.2023.1982717.1409

Abstract

Background: Ovarian hyperstimulation syndrome (OHSS) is one of the female reproductive disorders that can occur following the administration of injectable hormonal drugs to stimulate ovulation. Betaine (BET) is an intracellular biomolecule which anti-inflammatory and tissue protection effects. However, information about its effects in the experimental model of OHSS is not available. The current study aimed to investigate the possible effects of BET on abnormal expression of vasoconstrictor proteins and ovarian histological changes observed in experimental OHSS rat model.

Materials and Methods: In this experimental study, thirty adult female 2-month-old rats randomly were divided into 6 groups (n=5) including the control, OHSS (the eCG (10 IU, sc) for 4 days and then hCG (30 IU, sc) on the 5th day), OHSS+ BET (200 mg/kg/day, orally for 7 days), OHSS+ CAB (100 mg/kg/day, orally for 6 days), BET and CAB. The expression of vascular endothelial growth factor (VEGF), cyclooxygenase-2 (COX-2), and blood levels of estradiol (E2) and progesterone (P4) were measured at the end of the experiment. Also, the ovaries of the animals were studied for histomorphological alterations.

Results: Our results showed that OHSS induction altered tissue histology, including an increase in the number of corpus luteum and atretic follicles, while decreasing the number of follicular reserves. In this group seen increase of expressions of VEGF and COX-2 proteins and increased level of E2 and P4. The administration of CAB and BET could significantly attenuate all molecular and histological alterations observed in OHSS animals.

Conclusions: Our findings for first time, indicated the beneficial effects of BET to reduce the complications of OHSS in patients by reducing the expression of vasoactive proteins and also improving ovarian tissue changes, similar to CAB as chemical drug and can be a new gate to open more research in the future.

Keywords

International Journal of Fertility and Sterility

Articles in Press, Accepted Manuscript
Available Online from 23 December 2023

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