Document Type : Original Article
Authors
1
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3
Department of Biochemistry, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Kerman Regional Blood Transfusion
4
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
5
Department of Biochemistry, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
6
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
7
Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran
10.22074/ijfs.2023.1999395.1457
Abstract
Background: Endometriosis is an idiopathic gynecological condition affecting women with pelvic pain and infertility in reproductive ages. The aim of the current study was to evaluate the potential role of soy isoflavones (SI) and broccoli extract (BE) on the pathological scores of endometrioses and also consider the expression level of the gene markers of autophagy, apoptosis, and oxidative stress in endometriosis rat model.
Materials and methods: In the present experimental study, 45 mature female Sprague- Dawley rats weighing 220 ± 20gr at the age of eight weeks with surgically induced endometriosis was divided into five groups and treated for six weeks with normal saline (control group), BE, SI, BE+SI by oral gavage, and also Diphereline intramuscularly. The histopathology of the endometrial implants and the expression level of Bcl2, BAX, caspase-3, Beclin-1, LC3, and SOD within peritoneal tissue were compared among the groups.
Results: Pathologic scores of the implants in the Diphereline (1.2 ± 0.27) and BE+SI (1.2 ± 0.41) group were declined significantly compared to the control group (2.08 ± 0.44) (p ≤ 0.001). In the endometriotic structures, the expression of autophagy-related (LC3 and BECN1), apoptosis-related (CASP3, BAX, and Bcl2) and oxidative stress related genes was improved significantly (p ≤ 0.01) compared to the control group.
Conclusion: The findings of the current study demonstrate that the simultaneous consumption of a certain amount of broccoli extract and soy isoflavones can be considered as a promising therapeutic strategy for treatment of endometriosis.
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