Document Type : Short Communication
Authors
1
Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran. Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
2
Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran, P.O. Box 159163-4311
3
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
4
Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, P.O. Box 19395-4644
10.22074/ijfs.2023.1990652.1443
Abstract
Artificial ovary based on the alginate (ALG) hydrogel has been introduced to preserve prepubertal girls’ fertility. Amniotic membrane extract (AME), a rich supply of proteins and signaling factors, could be a promising component to ameliorate the ALG limitations. In this study, first, the impact of different concentrations of follicle-stimulating hormone (FSH) was evaluated on the mouse preantral follicles encapsulated in ALG. 100 mIU/ml FSH showed a significant follicle survival rate compared with 10 mIU/ml FSH (p<0.01). Later, the appropriate dose was adjusted for the follicles encapsulated in the ALG-AME hydrogel. According to MTT findings, the rate of weight loss, and rheology evaluations, ALG containing 1 mg/ml AME was identified as an optimal sample for follicle culture instead of other AME concentrations. Follicle diameter significantly increased in the ALG-AME 1 hydrogel compared with the ALG control group without AME (p<0.05). The storage modulus of ALG-AME 1 was 773 Pa and retained the follicle morphology for 13 days. No statistically substantial difference was seen in survival, antrum cavity formation, and competent oocyte in terms of the normal chromosomal arrangement and meiotic spindle rate in comparison with the control group. Consequently, ALG-AME 1 could not significantly impact the mouse preantral follicle.
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