Altered Expression of Circulating miR-223-3p in patients with idiopathic recurrent pregnancy loss

Articles in Press

Document Type : Original Article

Authors

1 Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran

2 Genetics Errors of Fertility Research Center, ART Center of ACECR East Azerbaijan Branch, East Azerbaijan ACECR, Tabriz, Iran

3 Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran

10.22074/ijfs.2023.2005351.1483

Abstract

objective: Circulating microRNAs have recently emerged as key regulators of a wide array of biological processes which are aberrantly expressed under physiological as well as pathological conditions. This study aimed to determine whether altered expression of the circulating miR-223-3p in plasma is associated with an increased risk of idiopathic recurrent pregnancy loss (iRPL), and could be novel non-invasive biomarkers for diagnosis of iRPL.

Materials and methods: Plasma samples were obtained from sixty women with a history of ≥ 2 consecutive miscarriages and sixty women with at least one full-term pregnancy and no history of miscarriage to assess the expression of the circulating miR-223-3p by quantitative real-time polymerase chain reaction (qPCR) procedure, and determine the correlation between patient clinico-demographic parameters and miR-223-3p expression levels. We also used receiver operating characteristic (ROC) curve analysis to evaluate the diagnostic accuracy of miR-223-3p in iRPL diagnosis.

Results: Our findings indicated that the expression level of miR-223-3p in patients with iRPL was significantly up-regulated compared with healthy controls (P = 0.002). No significant correlation was found between the expression level of miR-223-3p and number of abortions in iRPL patients (P = 0.344). ROC curve analysis revealed that the area under the curve (AUC) value for miR-223-3p was 0.658 (95% Confidence Interval (95% CI) = 0.559 - 0.757, P = 0.002).

Conclusion: These results suggest that a higher expression level of circulating miR-223-3p may be closely related with increased iRPL risk and may serve as a promising non-invasive diagnostic biomarker for iRPL.

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International Journal of Fertility and Sterility

Articles in Press, Accepted Manuscript
Available Online from 15 October 2023

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