Document Type : Original Article
Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Hormozgan Institute of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Medical Genetics and Prenatal Diagnostic Lab, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Background: Accumulating evidences suggest that date palm pollen (DPP) induces antioxidant activity and improves
semen parameters in male rats. However, there is a few scientific evidences in support of the DPP effects on human
male fertility. Hence, the effect of oral consumption of DPP on sperm parameters and expression pattern of Peroxiredoxin-
1 (PRDX1) and Peroxiredoxin-6 (PRDX6) genes was evaluated in men with infertility.
Materials and Methods: The current controlled clinical trial included 40 men with infertility (DPP group) and 10 normospermic
fertile men as controls. The DPP group received gelatinous capsules of DPP (400 mg/kg) for 74 days. Semen
sampling was done before and after treatment in the both groups. Semen analysis and 8-isoprostane concentration assessments
were performed by computer-assisted sperm analysis and ELISA methods, respectively. Quantitative reverse transcription
polymerase chain reaction (qRT-PCR) assays were employed to explore expression of PRDX1 and PRDX6 genes.
Results: DPP consumption significantly improved semen volume (P=0.030), count (P<0.001) and morphology of
sperm (P=0.023). Concentration of 8-isoprostane was significantly decreased after intervention in the DPP group
(P<0.001). DPP consumption led to a significant elevation in the expression of PRDX1 and PRDX6 genes (P<0.001).
Elevated gene expression of PRDX6 and PRDX1 was positively correlated with improved parameters of sperm including
count, volume, motility and morphology.
Conclusion: Taken together, DPP seems to promote sperm quality through a decrease in reactive oxygen species
(ROS) by increasing expression of antioxidant genes. Further large-scale studies are required to challenge this hypothesis
(registration number: IRCT2015021221014N2)