Effect of Low-Intensity Endurance Training and High-Intensity Interval Training on Sperm Quality in Male Rats with Fatty Liver

Document Type : Original Article

Authors

1 Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

2 Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Isfahan, Iran

3 Biology Department, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran

4 Department of Exercise Physiology, Faculty of Sport Sciences, Shahrekord University, Shahrekord, Iran

Abstract

Background: We aimed to investigate the effect of low-intensity endurance training (LIET) and high-intensity interval
training (HIIT) on sperm parameters, chromatin status, and oxidative stress in a rat model of non-alcoholic fatty
liver disease (NAFLD).

Materials and Methods: For this experimental study, we divided 40 male Wistar rats into four groups (control, sham,
HIIT and LIET) according to diet treatment and exercise training protocol. Liver triglycerides, sperm parameters,
sperm lipid peroxidation (BODIPY C11 probe) and chromatin status [chromomycin A3 (CMA3)], and acridine orange
[AO] staining) were assessed in these groups at the end of the study.

Results: The mean liver triglyceride values significantly improved in both the LIET and HIIT groups compared to
the control and sham groups. The mean of testicular volume, sperm concentration, motility, intensity of sperm lipid
peroxidation and DNA damage were similar within groups. While, the mean percentage of sperm lipid peroxidation
and protamine deficiency were significantly higher in the LIET and HIIT groups compared to the control group.

Conclusion: Both LIET and HIIT in the rat NAFLD model had no adverse effects on testicular morphometric parameters,
sperm concentration, motility, and DNA integrity. However, the mean sperm lipid peroxidation and protamine
deficiency were significantly higher in both exercise groups. Our study suggests that exercise or antioxidant supplementation
could minimise the adverse effects of oxidant by-products of exercise.

Keywords

References

1. Durairajanayagam D. Lifestyle causes of male infertility. Arab J Urol. 2018; 16(1): 10-20.
2. Keltz J, Zapantis A, Jindal SK, Lieman HJ, Santoro N, Polotsky AJ.Overweight men: clinical pregnancy after ART is decreased in IVF but not ICSI cycles. J Assist Reprod Genet. 2010; 27(9-10): 539-544.
3. Li L, Law C, Lo Conte R, Power C. Intergenerational influences on childhood body mass index: the effect of parental body mass index trajectories. Am J Clin Nutr. 2009; 89(2): 551-557.
4. Bechmann LP, Hannivoort RA, Gerken G, Hotamisligil GS, Trauner M, Canbay A. The interaction of hepatic lipid and glucose metabolism in liver diseases. J Hepatol. 2012; 56(4): 952-964.
5. Benhamed F, Denechaud Pd, Robichon C, Moldes M, Girard J, Lemoine M, et al.The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans. J Clin Invest. 2012; 122(6): 2176-2194.
6. van der Windt DJ, Sud V, Zhang H, Tsung A, Huang H. the effects of physical exercise on fatty liver disease. Gene Expr. 2018; 18(2): 89-101.
7. Kirk EP, Reeds DN, Finck BN, Mayurranjan MS, Patterson BW, Klein S. Dietary fat and carbohydrates differentially alter insulin sensitivity during caloric restriction. Gastroenterology. 2009; 136(5): 1552-1560.
8. MacDonald AA, Herbison GP, Showell M, Farquhar, CM. The impact of body mass index on semen parameters and reproductive hormones in human males: a systematic review with meta-analysis. Hum Reprod Update. 2010; 16(3): 293-311.
9. La Vignera S, Condorelli RA, Vicari E, Calogero AE. Negative effect of increased body weight on sperm conventional and nonconventional flow cytometric sperm parameters. J Androl. 2012; 33(1): 53-58.
10. Vaamonde D, Da Silva ME, Poblador MS, Lancho JL. Reproductive profile of physically active men after exhaustive endurance exercise. Int J Sports Med. 2006; 27(9): 680-689.
11. Hajizadeh Maleki B, Tartibian B. Moderate aerobic exercise training for improving reproductive function in infertile patients: a randomized controlled trial. Cytokine. 2017; 92: 55-67.
12. Maleki BH, Tartibian B. High-intensity exercise training for improving reproductive function in infertile patients: a randomized controlled trial. J Obstet Gynaecol Can. 2017; 39(7): 545-558.
13. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd ed. Oxford: Oxford University Press; 1999; 1-25.
14. Alessio HM. Exercise-induced oxidative stress. Med Sci Sports Exerc. 1993; 25(2): 218-224.
15. Oztasan N, Taysi S, Gumustekin K, Altinkaynak K, Aktas O, Timur H, et al. Endurance training attenuates exercise induced oxidative stress in erythrocytes in rat. Eur J Appl Physiol. 2004; 91(5-6): 622-627.
16. Poulsen HE, Loft S, Vistisen K. Extreme exercise and oxidative DNA modification. J Sports Sci. 1996; 14(4): 343-346.
17. Cho J, Koh Y, Han J, Kim D, Kim T, Kang H. Adiponectin mediates the additive effects of combining daily exercise with caloric restriction for treatment of non-alcoholic fatty liver. Int J Obes (Lond). 2016; 40(11): 1760-1767.
18. Leandro CG, Levada AC, Hirabara SM, Manhães-de-Castro R, De-Castro CB, Curi R, et al. A program of moderate physical training for Wistar rats based on maximal oxygen consumption. J Strength Cond Res. 2007; 21(3): 751-756.
19. Smith-Ryan AE, Melvin MN, Wingfield HL. High-intensity interval training: Modulating interval duration in overweight/obese men. Phys Sportsmed. 2015; 43(2): 107-113.
20. Bagheri MH, Azamian Jazi A, Bani Talebi E, Nasr-Esfahani MH. The effects of eight weeks of high intensity interval training on expression of PPARγ and liver TG in rats with fatty liver disease. Sport Physiology. 2020; 12 (47): 113-132.
21. Afiyani AA, Deemeh MR, Tavalaee M, Razi M, Bahadorani M, Shokrollahi B, et al. Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction. Mol Reprod Dev. 2014; 81(8): 766-776.
22. Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA. Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod. 2007; 13(4): 203-211.
23. Sermondade N, Faure C, Fezeu L, Shayeb AG, Bonde JP, Jensen TK, et al. BMI in relation to sperm count: an updated systematic review and collaborative meta-analysis. Hum Reprod Update. 2013; 19(3): 221-231.
24. Edmonds ES, Dallie SK, Withyachumnarnkul B. Reproductive system of the obese male zucker rat. Reproductive capacity, artificial insemination and plasma testosterone levels. Biol Reprod. 1982; 27(4): 891-897.
25. Joseph AM, Nguyen LM, Welter AE, Dominguez JM, Behnke BJ, Adhihetty PJ. Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats. Biogerontology. 2014; 15(5): 517-534.
26. Dominguez JM, Davis RT, McCullough DJ, Stabley JN, Behnke BJ. Aging and exercise training reduce testes microvascular PO2 and alter vasoconstrictor responsiveness in testicular arterioles. Am J Physiol Regul Integr Comp Physiol. 2011; 301(3): R801-R810.
27. Santos M, Rodríguez-González GL, Ibáñez C, Vega CC, Nathanielsz PW, Zambrano E. Adult exercise effects on oxidative stress and reproductive programming in male offspring of obese rats. Am J Physiol Regul Integr Comp Physiol. 2015; 308(3): R219-R225.
28. Palmer NO, Bakos HW, Owens JA, Setchell BP, Lane M. Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function. Am J Physiol Endocrinol Metab. 2012; 302(7): E768-E780.
29. Yi X, Gao H, Chen D, Tang D, Huang W, Li T, et al. Effects of obesity and exercise on testicular leptin signal transduction and testosterone biosynthesis in male mice. Am J Physiol Regul Integr Comp Physiol. 2017; 312(4): R501-R510.
30. Nematollahi A, Kazeminasab F, Tavalaee M, Marandi SM, Ghaedi K, Nazem MN, et al. Effect of aerobic exercise, low-fat and high-fat diet on the testis tissue and sperm parameters in obese and nonobese mice model. Andrologia. 2019; 51(6): e13273.
31. Chigurupati S, Gen Son T, Hyun DH, Lathia JD, Mughal MR, Savell J, et al. Lifelong running reduces oxidative stress and degenerative changes in the testes of mice. J Endocrinol. 2008; 199(2): 333-341.
32. Gomes M, Freitas MJ, Fardilha M. Physical activity, exercise, and mammalian testis function: emerging preclinical protein biomarker and integrative biology insights. OMICS. 2015; 19(9): 499-511.
33. Tartibian B, Hajizadeh Maleki B. Correlation between seminal oxidative stress biomarkers and antioxidants with sperm DNA damage in elite athletes and recreationally active men. Clin J Sport Med. 2012; 22(2): 132-139.
34. Kawamura T, Muraoka I. Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants (Basel). 2018; 7(9): E119.
35. Aksoy Y, Yapanoğlu T, Aksoy H, Demircan B, Oztaşan N, Canakçi E, et al. Effects of endurance training on antioxidant defense mechanisms and lipid peroxidation in testis of rats. Arch Androl. 2006; 52(4): 319-323.
36. Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Hum Reprod. 2010; 25(10): 2415-2426
37. Xavier MJ, Nixon B, Roman SD, Scott RJ, Drevet JR, Aitken RJ. Paternal impacts on development: identification of genomic regions vulnerable to oxidative DNA damage in human spermatozoa. Hum Reprod. 2019; 34(10): 1876-1890.
38. Kovac JR, Addai J, Smith RP, Coward RM, Lamb DJ, Lipshultz LI. The effects of advanced paternal age on fertility. Asian J Androl. 2013; 15(6): 723-728.
39. Rima D, Shiv BK, Bhavna Ch, Shilpa B, Saima Kh. Oxidative stress induced damage to paternal genome and impact of meditation and Yoga - can it reduce incidence of childhood cancer? Asian Pac J Cancer Prev. 2016; 17(9): 4517-4525.
40. López-Lemus UA, Garza-Guajardo R, Barboza-Quintana O, Rodríguez-Hernandez A, García-Rivera A, Madrigal-Pérez VM, et al. Association between nonalcoholic fatty liver disease and severe male reproductive organ impairment (germinal epithelial loss): study on a mouse model and on human patients. Am J Mens Health. 2018; 12(3): 639-648.
International Journal of Fertility and Sterility
Volume 15, Issue 2
April 2021
Pages 141-147

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