Document Type : Original Article
Authors
Abstract
Keywords
The first recognizable structure inside the gestational sac is the yolk sac, which should be detectable as a regularly rounded extra-amniotic structure when the gestational sac reaches dimensions of 8 to 10 mm. The normal biometric value of the yolk sac diameter during the first trimester should be an inner diameter of 3-6 mm.
Spontaneous abortion has been noted to occur
in cases with no yolk sac, a yolk sac with dimensions
over 6 mm or under 3 mm, an irregular shape
(mainly wrinkled with indented walls), the presence
of degenerative changes such as numerous
calcifications that have decreased translucency
of the yolk sac and the yolk sac number, which
should be the same as the number of embryos as
visualized by 2-D ultrasound (
In a normal pregnancy it should be possible to observe the yolk sac between 5-12 weeks of pregnancy
or when it reaches 10 mm in size. Abnormal morphological
appearance of the yolk sac and/or a size
over 9 mm is suggestive of serious growth disorders
of the fetus (
Pregnancies that have a mean yolk sac diameter
equal or larger than 5 mm on early ultrasound are
also associated with a threefold increased risk for
first trimester loss, independent of maternal risk
factors such as age, body mass index, polycystic
ovary syndrome, smoking, and diabetes (
The lack of a yolk sac or a smaller than gestational
age yolk sac diameter are indicative of pregnancies
that may result in spontaneous abortion (
This study was undertaken to determine if there were different abortion rates between normal and abnormal yolk sacs.
This study was a cohort study carried out on 193 pregnant women who were between 5-6.5 weeks of gestation and with a body mass index (BMI) between 18-24. Patients were seen in Amir University Hospital, Semnan, Iran during May 2009 to May 2010. The method of sampling was convenient. Written informed consent was obtained from all patients. This study was approved by the Ethical Committee of Semnan University of Medical Sciences.
The following patients were excluded: those whose gestational age was more than 6.5 weeks, patients with diabetes, hypertension, or any systemic disease, those who expressed unwillingness to come for follow up visits, and known cases of Mϋllerian anomalies.
We performed two-dimensional transvaginal ultrasonography on 191 consecutive pregnant cases that were between 5-6.5 weeks of gestation, as part of the routine evaluation or other indication for ultrasonography. The size of the yolk sac (inner to inner diameter), shape, echogenicity of rim and center of sac, numbers of yolk sac and degenerative changes such as calcification were evaluated. For all cases, the two-dimensional transvaginal ultrasonography was performed by the same sonographer.
Yolk sacs that had the following characteristics were classified as normal: diameter between 2-5 mm, round shape, absence of degenerative changes, equal number with embryos, the presence of an echogenic rim and hypo-echoic center. Yolk sacs that had diameters smaller than 2 mm or larger than 5 mm, were not round (i.e., oval or distorted), had evidence of degenerative changes, hyper- or hypoechogenic rim, hyperechoic center, and unequal number with embryos were considered abnormal.
Based on the above mentioned criteria patients were divided into two groups, study (abnormal yolk sac) and control (normal yolk sac).
All cases were followed until delivery and the abortion rates (delivery before 20 weeks of gestation) were compared between the groups.
Data were expressed as percentage and compared using the chi-square and students’ t tests. Statistical analyses were performed with the Statistical Package for Social Sciences (SPSS, version 16.0).
The mean age of the study group was 28.1 ± 5.8 years and that of the control group was 28 ± 4.8 years, which was not statistically significant (p=0.876). During the study period a total of 193 cases were evaluated. There were two cases who did not return for their follow up visits, therefore we excluded them from the study. Out of 191 cases that were evaluated, 22 (11.51%) had abnormal yolk sacs of which spontaneous abortion occurred in 14 (63.63%). The control group consisted of 169 cases. Of these, spontaneous abortion occurred in 6 (3.55%). There was a statistically significant difference in abortion rates between the two groups (p=0.000).
With regards to abnormal yolk sac characteristics,
11 (50%) had yolk sacs that were greater
than 5 mm; the largest was 9.2 mm in a patient
who had four recurrent abortions (
The largest yolk sac in a case with recurrent abortion.
Frequency of abortion rates of the study group based on yolk sac characteristics.
Characteristics | Number (%) | Abortion rates |
---|---|---|
11 (50) | 10 (45.45) | |
4(18.18) | 2(9.9) | |
4(18.18) | 1(4.54) | |
1(4.54) | 0 | |
1(4.54) | 1(4.54) | |
1 (4.54) | 0 | |
Four (18.18%) had distorted yolk shape with
spontaneous abortion in 2 (9.90%) who were between
6-8 weeks of pregnancy (
A distorted yolk sac (a case of abortion in this study).
One (4.54%) case had two yolks, however her
pregnancy was uneventful. In 1 (4.54%) case, no
yolk sac was present and a spontaneous abortion
occurred at nine weeks of pregnancy (
One (4.54%) case had two yolks, however her
pregnancy was uneventful. In 1 (4.54%) case, no
yolk sac was present and a spontaneous abortion occurred
at nine weeks of pregnancy (
In 4 (18.18%) patients, the yolk sacs had hypo-echoic
rims; all had long-term bleeding during the first trimester
of pregnancy which was stopped by the use of progesterone
preparations. With the exception of one abortion
at seven weeks of pregnancy, the remaining pregnancies
continued to term. In one case of this group, the pregnancy
was terminated at 34 weeks because of oligohydramnios,
but the fetus was normal and had no problems
after delivery (
In the control group, abortion occurred in 6 (3.55%) cases. Pregnancy continued to term in the remaining control cases (n=163; 96.44%). Preterm delivery occurred in 7 control patients; however all were more than 33 weeks pregnant. Of these, all neonates survived.
With regards to yolk sac characteristics of the control group, the sac diameters ranged from 2-4.9 mm, all of which had a round shape.
The yolk sac is the first structure of the gestational
sac, which must be present when the mean
gestational sac diameter is 13 mm or smaller in size
(
In this study we evaluated the characteristics of the first important structure of the gestational sac and its relation to the spontaneous abortion rate.
Pregnancies that have a mean yolk sac diameter
equal or larger than 5 mm as visualized on early ultrasound
are associated with a threefold increased risk of
first trimester loss (
In agreement with the above studies, the results of this study showed that yolk size was an important factor for prediction of spontaneous abortion. Yolk sacs larger than 5 mm at 5-6.5 weeks of pregnancy were a good indication that the probability of abortion was significantly high.
However, a very large yolk sac may exist in normal
pregnancy and the presence of a yolk sac with
a diameter of 8.1 mm in a viable pregnancy has been reported (
Larger yolk sac diameters may represent evidence
of certain diseases and the pregnancy loss in these
pregnancies is reflective of the presence of such underlying
diseases. According to Ivanisević et al. in
their study the researchers confirmed the presence
of larger yolk sac diameters amongst type 1 diabetic
women who were over six weeks of gestation (
Abnormal yolk sac shape are associated with abnormal
pregnancy outcome with a sensitivity of 29%,
specificity of 95%, positive predictive value of 47%
and negative predictive value 90.5% (
Failure to detect the presence of a yolk sac before
the detection of an embryo in the first trimester by
ultrasound is suggestive of an abnormal intrauterine
pregnancy (
Usually there is one yolk sac in a single pregnancy
and the number of sacs must equal the number of
embryos. During the first trimester of a dichorionic
twin pregnancy the yolk sacs are always separated
by a septum. They are not separated in a monochorionic
twin pregnancy (
Usually a normal yolk sac has an echogenic rim and hypoechoic center. In the current study there were 4 (18.18%) with hypoechoic rims, all experienced long term bleeding in the first trimester, however only one case had an abortion. It was probable that this particular characteristic of the yolk sac was not an important factor for an abortion.
We have concluded that abnormal yolk sac characteristics are associated with spontaneous abortion. In this study abortion occurred in 90.9% of cases where the yolk sac was of an abnormal size and in 50% of those with distorted shape yolk sacs.
Thus, it is presumed that among the yolk sac characteristics, a large-size yolk and distorted shape are the most important factors for early pregnancy loss. Evaluation of the importance of other criteria needs additional studies with larger numbers of cases.