Document Type : Original Article
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Background: In many diabetic patients, spermatogenesis complications are frequent causing infertility problems.
This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.
Materials and Methods: In this experimental study, type 2 diabetes was induced by a high-fat diet (HFD) for one
month and then a low single dose injection (35 mg/kg) of streptozotocin (STZ) in Wistar rats. After 72 hours, rats with
more than 200 mg/dl of blood glucose were considered type 2 diabetic rats. Forty rats (200-250 g) were divided into
four groups (n=10) including group 1 (G1): rats with normal diet and buffer citrate (STZ solvent) injection, group 2
(G2): control type 2 diabetic rats with HFD and STZ injection, group 3 (G3): type 2 diabetic rats received phosphate
buffer saline (PBS) as Forskolin solvent, and group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month.
Results: In comparison to control group, in diabetic groups (G2 and G3) some parameters are increased significantly:
The blood glucose (P=0.00078), testicular malondialdehyde (MDA) level and body weight (P=0.00009) and Bax
gene expression (P=0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone
(P=0.0009), testicular superoxide dismutase (SOD, P=0.00007) and glutathione peroxidase (GPX) levels (P=0.00008),
sperm concentration (P=0.00008), motility (P=0.00009), normal morphological sperm (P=0.00008) and Bcl-2 gene
expression (P=0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values that
was significantly (P=0.00007) higher than in G2 and G3 groups. Therefore, treatment with Forskolin significantly
improved these abnormal changes in Forskolin-treated group.
Conclusion: Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improves
sperm concentration, testosterone levels, and antioxidant activity in diabetic rats.