Document Type : Original Article
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Background: In many diabetic patients, spermatogenesis complications are frequent and cause infertility problems. This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.
Materials and methods: In this study, type 2 diabetes was induced by a high-fat diet (HFD) for one month and then a low single dose injection (35 mg/kg) of Streptozotocin (STZ) in Wistar rats. After 72 hours, rats with more than 200 mg/dl of blood glucose were considered type 2 diabetic rats.
Forty rats (200-250 grams) were divided into four groups (n=10) including Group 1 (G1): rats with normal diet and buffer citrate (STZ Solvent) injection, Group 2 (G2): control type 2 diabetic rats with HFD and STZ injection, Group 3 (G3): type 2 diabetic rats received phosphate buffer saline (PBS) as Forskolin solvent, and Group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month.
Results: In comparison to control group, in diabetic groups (G2 & G3) some parameters are increased significantly: The blood glucose (p=0.00078), testicular MDA level and body weight (p = 0.00009) and Bax gene expression (p = 0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone (p = 0.0009), testicular SOD (p = 0.00007) and GPX levels (p = 0.00008), sperm concentration (p = 0.00008), motility (p = 0.00009), normal morphological sperm (p = 0.00008) and Bcl-2 gene expression (p = 0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values and was significantly (p = 0.00007) higher than in G2 & G3 groups. Therefore, treatment with Forskolin was significantly improved this abnormal changes in Forskolin-treated group.
Conclusion: Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improves sperm concentration, testosterone levels, and antioxidant activity in diabetic rats.