USP7 and SET9 Expression in Human Oligospermic Semen

Document Type : Original Article


Department of Biology, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran



Background: Oligospermia is a condition in which the number of sperm in each ejaculation is less than 15 million per milliliter of semen. Proper and complete spermatogenesis requires the expression of a large number of genes. As a result stopping the expression of any of them may lead to disrupting the process of spermatogenesis. In order to understand the disorders of spermatogenesis, it is necessary to study genes whose expression is effective in the process of spermatogenesis. Therefore, in the present study, USP7 and SET9 (SETD7) gene expression was examined in the healthy and oligospermic men.

Materials and Methods: Semen samples of individuals with normal sperm and oligospermia were collected from men who referred to Roya clinic according to WHO parameters after obtaining consent. Then the expression of USP7 and SET9 genes in two groups were analyzed using quantitative polymerase chain reaction (qPCR). Results: There was no difference between the age of healthy and oligospermic individuals (p=0.889). The data showed that, USP7 gene expression in patients was 3.99 times higher than control group (P<0.001). The expression of SET9 gene in patient was 1.28 times less than control, which was not significant (P = 0.231).

Conclusion: The results indicated that USP7 gene expression was increased in 84% of oligospermic individuals. Therefore, this gene can be considered as one of the molecular markers in the development of oligospermia.


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