Risk factors associated with recurrent pregnancy loss and outcome of pre-implantation genetic screening of affected couples

Document Type : Original Article

Authors

1 2. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 6. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute

2 1. Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran. 2. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive

3 2. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 3. Department of Molecular Genetics, Faculty of Basic Sciences and Advanced Technologies in Biology,

4 Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

5 Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, Iran.

6 5. Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

7 1. Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran.

10.22074/ijfs.2021.137626.1027

Abstract

Background: Recurrent pregnancy loss (RPL) is a multifactorial disorder which affects up to 5% of couples. Several factors are considered to be involved in RPL; however, in 35–60% of cases, the etiology remains unexplained. The aim of this study was to assess the frequency of risk factors associated with this phenomenon as well as pre-implantation genetic screening (PGS) outcomes achieved by array comparative genomic hybridization (array-CGH) and fluorescence in situ hybridization (FISH), in 602 Iranian couples with RPL.
Materials and Methods: Clinical data were analyzed to assess possible associations between various factors and RPL. Karyotyping was performed using standard cytogenetic techniques. PGS using FISH and array-CGH was applied for pre-implantation embryos of the RPL patients.
Results: Data analysis revealed a frequency of 15.61% chromosomal abnormalities in RPL couples. Also, the reciprocal translocations were more frequent (33/1204 cases) compared to the other structural abnormalities. In couples with normal karyotype, the live birth rate after PGS, as assessed by array-CGH and FISH, was 70.37 and 55%, respectively.
Conclusion: Our findings could confirm a positive correlation between chromosomal abnormalities and RPL. In addition, applying PGS for the patients who suffer from RPL leads to improvement of pregnancy success rate.

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