Document Type : Original Article
Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra Institute of Higher Education and Research, Chennai, India
Background: Polycystic ovary syndrome (PCOS) and oral health are found to share a reciprocal link. Previous substantiating
evidences suggest that PCOS may have a confounding effect on periodontal health and may quantitatively modify
the composition of the oral microbiome. To analyze the role of PCOS as a risk factor in causing periodontal disease, we
compared and evaluated the levels of Porphyromonas gingivalis and Fusobacterium nucleatum in patients with polycystic
ovary syndrome, polycystic ovary syndrome, chronic periodontitis, polycystic ovary syndrome, and gingivitis, and
Materials and Methods: In this case-control study, 40 female participants are enrolled and grouped into four groups
which included healthy female individuals, patients with PCOS, patients with PCOS and gingivitis, and patients with
PCOS and periodontitis. Periodontal examination is assessed primarily on all the participants using a UNC-15 probe.
Dental plaque is then collected using a sterile curette in one stroke and transferred into an Eppendorf tube containing
TE Buffer (Tris-EDTA buffer) solution. The level of Porphyromonas gingivalis and Fusobacterium nucleatum was
estimated by real-time polymerase chain reaction (PCR).
Results: The levels of Fusobacterium nucleatum were observed to be significantly higher in group with patients with
PCOS and periodontitis.
Conclusion: PCOS may have an impact on the composition of oral microflora causing repercussions in periodontal