Cryopreservation of a small number of sperm using taurine antioxidant and thawing in two different temperatures and devices

Document Type : Original Article


1 Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

2 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran



Background: Sperm cryopreservation plays a vital role in maintaining the fertility of couples undergoing infertility treatment, specifically in patients with limited sperm numbers. This study was performed to compare the effect of sucrose (S) and taurine antioxidant (T) compounds against commercial cryoprotectant (CPA) during individual human sperm cryopreservation on cryotop and thawing at 37 °C and 42 °C.

Materials and Methods: In this experimental study, progressively motile sperm were selected using an inverted microscope after sample processing by the "Swim-up" method. Sperm were transferred into droplets of the freezing medium, including "sucrose medium" with 50 mM Taurine antioxidant (S+T) and commercial cryoprotectant "Sperm Freeze" (CPA), located on a petri dish and cryotop. Following rapid freezing, the sample was thawed at two temperatures (37 °C and 42 °C), and sperm parameters, viability, and DNA fragmentation were examined.

Results: Statistical analysis displayed a significant increase in total and progressive motility in individual sperm freezing on cryotop with CPA and thawing at 42°C (p<0.05). Other parameters did not show any differences between the CPA and S+T group and two thawing temperatures in either of the cryopreservation methods.

Conclusion: Although, both cryoprotectants (CPA and S+T) may preserve individual sperm effectively using cryotop, the CPA and thawing at 42 °C showed a better effect on motility percentage of the small number of sperm.


Main Subjects