The Correlation of Urine Bisphenol A with Semen Parameters in Men Referred to Infertility Centers: A Cross-Sectional Study

Document Type : Original Article


1 Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

2 Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran


Background: Bisphenol A (BPA) is known as an endocrine disruptor that has harmful effects on general health. It is
commonly used in various industrial products. In this study we tried to evaluate the amount of BPA in urine samples
of the men referred to an infertility center.
Materials and Methods: The cross-sectional study population consisted of male partners of infertile couples, who were
referred to infertility clinic in Mazandaran, a northern state of Iran. Questionnaires included demographic characteristics,
medical history, lifestyle factors, physical examinations. A semen sample and a spot urine sample were taken from each
participant. In the initial study group of 240 men, 3 groups were excluded, and 122 men remained for the analysis. Highperformance
liquid chromatography (HPLC) was applied to measure the amount of BPA in the urine samples.
Results: BPA was not detected in about half of the samples (53.3%). Multiple linear regression analysis showed that
no significant relationship existed between the urine concentrations of BPA, semen parameters and male reproductive
hormones. However, in a comparison with semen parameters in people with detectable urine BPA versus nondetectable
ones, an inverse association was noticed with sperm concentration. In other parameters, differences were not
significant. Smoking had no effects on sperm parameters, but body mass index (BMI) ≥25 reduced the percentage of
normal sperm parameters.
Conclusion: In most participants, urinary BPA was not detected. Probably in this study low environmental exposure to
BPA is the cause of lower urine BPA concentrations compared to other industrially developed countries. Therefore, no
overall relationship was observed between BPA level and male infertility.


Main Subjects


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