Twelve Hours Post-Injection Serum Human Chorionic Gonadotropin and Body Mass Index Predicts In Vitro Fertilization Oocyte Maturation Rate: A Cross-Sectional Study

Document Type : Original Article


1 Reproductive Immunoendocrinology Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia

2 Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia

3 Human Reproduction, Infertility, and Family Planning Cluster, Indonesia Reproductive Medicine Research and Training Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

4 Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia


Background: In vitro fertilization (IVF) remains a main treatment for infertility cases. Post-injection human chorionic gonadotropin
(hCG) level is an essential factor in determining oocyte maturation rate in IVF. This study aimed to determine the
relationship between 12 hours post-injection serum hCG level and oocyte maturation rate among IVF participants.
Materials and Methods: A cross-sectional study on IVF participants was done at a tertiary hospital in Indonesia from
January 2020 to December 2021. Subjects were injected with 250 μg of recombinant-hCG (r-hCG) subcutaneously.
Twelve hours post-injection serum hCG levels and oocyte maturation rate data were retrieved and analyzed accordingly.
Results: Twenty-eight subjects were recruited into the study. Higher 12 hours post-injection serum hCG was related
to oocyte maturation rate (P=0.046). The cut-off point of 12 hours post-injection serum hCG to predict better oocyte
maturation rate was 90.15 mIU/mL (sensitivity 68.2%, specificity 83.3%). Oocyte maturation rate may be predicted
using body mass index (BMI) and 12 hours post-injection serum hCG.
Conclusion: Higher 12 hours post-injection serum hCG was associated with a higher oocyte maturation rate in IVF



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