Document Type : Original Article
Ferticenter IVF Unit, Istanbul, Turkey . Ferticenter IVF Unit, Istanbul, Turkey Department of Biology, Gebze Institute of Technology, Kocaeli, Turkey . Department of Biology, Gebze Institute of Technology, Kocaeli, Turkey
Department of Biology, Gebze Institute of Technology, Kocaeli, Turkey
Ferticenter IVF Unit, Istanbul, Turkey
Zeynep Kamil Hospital, Istanbul, Turkey
Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmentation levels (DFLs) of patients.
Materials and methods
The DFLs were determined by Halosperm, a new improved sperm chromatin dispersion (SCD) test. Patients were grouped as low DNA fragmentation group (LFG ≤30%) and high fragmentation group (HFG >30%).
Our analysis showed that semen parameters including concentration of untreated sperm and motility of prepared semen were low in HGF, whereas other parameters were not different. Sperm DNA fragmentation levels decreased in both groups after semen preparation by density gradient technique.
No difference was detected on ICSI outcomes (fertilization, embryo development, embryo cleavage, embryo quality and pregnancy rates) between two group.