Document Type : Original Article
Genetics Department, Urmia University of Medical Sciences, Urmia, Iran
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
Obstetrics and Gynecology Department, Urmia University of Medical Sciences, Urmia, Iran
To assess whether the C677T and A1298C mutations in the methylenetetrahydrofolate reductase (MTHER) gene are associated with recurrent abortion (RA), we determined the frequencies of the T677 and C1298 mutations in patients and controls.
Materials and methods
Mutations were determined by a RFLP-PCR method in 53 patients and 61 matched controls.
The frequencies of T alleles were 0.26 in patients and 0.29 in controls. The frequencies of C/C, T/C and T/T genotypes were 34 (55.7%), 22 (36.1%) and 5 (8.2%) in patients, and 27 (50.9%), 21 (39.6%) and 5 (9.43%) in controls. The C allele frequencies were 0.38 in patients and controls. C/C, A/C and A/A genotype distributions were 9 (14.8%), 28 (45.9%) and 24 (39.3%) in patients, and 8 (15.1%), 24 (45.3%) and 21 (39.6%) in controls.
There were no significant differences between patients and controls concerning the T677 and C1298 mutations.