Detection and Evaluation of Macrolide Resistance (Erythromycin) in Mycoplasma hominis Isolated from Endocervical Specimens of Patients Referring to Ibn Sina Infertility Treatment Centre, Tehran, Iran

Document Type : Original Article


1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran

2 Nanobiotechnology Research Centre, Iranian Academic Centre for Education, Culture and Research, Tehran, Iran


Background: Mycoplasma hominis (M. hominis) is an important cause of bacterial infections of the genital tract. Macrolides are the first selective agents used to treat mycoplasma infections. However, widespread use of macrolides has led to a rapid and global emergence of macrolide-resistant strains. We evaluated macrolide resistance in M. hominis
isolated from endocervical specimens of patients who referred to Ibn Sina Infertility Centre in Tehran, Iran.
Materials and Methods: In this cross-sectional descriptive-analytical study, 160 samples of Dacron endocervix  swabs (80 infertile patient samples and 80 healthy controls) were collected and transferred to the laboratory. All samples were cultured in liquid pleuropneumonia-like organisms (PPLO) broth and PPLO agar solid media. After culturing and genome extraction, polymerase chain reaction (PCR) was performed using specific primers. Then, minimum inhibitory concentration (MIC) was obtained using the broth microdilution method. The MIC was recorded and reported for all samples positive for M. hominis against erythromycin.
Results: From the 160 endocervical specimens cultured in PPLO agar medium, 19 cases (23.75%) were positive. A total 
of 35 cases (42.5%) were positive using specific primers of M. hominis species. MIC results from all samples positive for 
M. hominis were measured against erythromycin. All of the M. hominis samples were resistant to erythromycin.
Conclusion: The results of the present study showed that a significant percentage of infertile women were infected 
with M. hominis. Also, MIC results from the broth microdilution method indicated that all strains positive for M. hominis were also resistant to erythromycin.


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