The Impact of Chrysin on The Folliculogenesis and Ovarian Apoptosis in Ischemia-Reperfusion Injury in The Rat Model

Document Type : Original Article

Authors

1 Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran.

2 Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran

3 Deartment of Obstetrics and Gynecology, Gonabad University of Medical Sciences, Gonabad, Iran

4 Deartment of Anatomy, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

5 Clnical Research Development Unit of Tabriz Valiasr Hospital, Tabriz University of Medical Sciences, Tabriz, Iran

6 Deartment of Biochemistry, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

7 Deartment of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

8 Nusing Research Center, Gonabad University of Medical Sciences, Gonabad, Iran

Abstract

Background: The ovarian Ischemia/reperfusion is one of the gynecological emergency concerns that may lead to the ovary damage and folliculogenesis. The present research aimed to evaluate the impact of the Chrysin (CH) on the ischemia-reperfusion (I/R) injury in the rat model.
Materials and Methods: In this experimental research, 48 adult female rats, 8 weeks age and 180-200 g weight, have been categorized into 6 equal groups (n=8) including one sham and 5 ovarian torsion groups (OT+CH groups) that received different treatments. Each group has been treated 30 min before detorsion with gavage of CH or normal saline for 1 week and pregnant mare serum gonadotropin (PMSG) has been injected on the day 5 for initiating folliculogenesis. Finally, bio-chemical, molecular, histopathological, apoptotic and hormonal evaluations were performed.
Results: The anti-oxidant enzyme, superoxide dismutase and glutathione peroxidase, ameliorated in the ovarian tissues of the OT+CH groups in comparison with the OT group (P

Keywords

References

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International Journal of Fertility and Sterility
Volume 16, Issue 4
October 2022
Pages 299-305

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