Document Type : Original Article
Department of Reproductive Biology, Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran
Department of Biology, Faculty of Science, Islamic Azad University, Qom, Iran
Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran
Background: It is thought that genetic factors are influential in the etiology of polycystic ovarian syndrome (PCOS),
the most frequent endocrinological disorder of females in their reproductive age. This study was carried out to elucidate
the association of rs13429458 and rs12478601 single nucleotide polymorphisms (SNPs) of the THADA gene and
the risk of the PCOS among a population of Iranian female patients.
Materials and Methods: This case-control study contains 66 infertile women with PCOS (patient group) and 44
healthy women without PCOS (control group) that referred to the IVF Unit of the Infertility Research Center of the
Academic Center for Education, Culture and Research (ACECR). The polymerase chain reaction (PCR) was utilized
to amplify genome DNA as well as direct sequencing to determine SNPs. The THADA rs12478601 and rs13429458
genotypes were consequently examined with amplification refractory mutation system-PCR (ARMS-PCR).
Results: In this study, we observed that rs13429458 polymorphism was not associated with PCOS risk in two groups (P=0.42). On the other hand, data analysis indicated that the rs12478601 genotype significantly increased the risk of PCOS in the case group (P=0.032) in compared with control group. We found that the “T” allele of rs12478601 in the THADA gene had a significant relation to PCOS in the case group (odds ratio [OR]: 2.574, 95% confidence interval [CI]: 1.439-4.604, P=0.001).
Conclusion: This study has presented further evidence that TT and CT genotype of THADA rs12478601 is associated
with a high risk of PCOS.