Protective effect of Minocycline on Bax and Bcl-2 gene expression, histological damages and oxidative stress induced by ovarian torsion in adult rats, as an animal experimental study.

Document Type : Original Article

Authors

1 Department of clinical Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.

2 Department of Biochemistry and Nutrition, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.

3 Department Of Anatomical Sciences, Faculty Of Medicine, Tabriz University Of Medical Sciences, Tabriz, Iran.

4 Department of Anatomy, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

5 Department of Obstetrics and Gynecology, School of Medicine, Allameh Bohlool Gonabadi Hospital, Gonabad University of Medical Sciences, Gonabad, Iran

6 Department of Physiology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

10.22074/ijfs.2021.522550.1069

Abstract

Background: The aim of this study is the investigation of apoptotic related gens in ovary of the rat model of torsion/detorsion treated by Minocycline.

Materials and Methods: This experimental study performed on 32 female Wistar rat which classified into 4 group;1 sham; 2 TD: torsion/detorsion group received normal saline; 3 TDM: torsion/detorsion group treated with 40mg/kg Minocycline; 4 MC: healthy group received 40mg/kg Minocycline. After the therapy period (7days), histoplogical parameters, the oxidative stress markers in serum and the expression of apoptotic related genes were measured in the ovary of rats.

Results: The level of SOD, GPX was decreased in the TD group. And also the level of MDA increased in the TD group and decreased in treated groups (p<0.001). The expression of Bax was elevated in the TD group although in treated groups their expression ratio was attenuated.

Conclusion: Our result shows that the administration of Minocycline can replace the oxidative damage in ovarian tissue and regulate the apoptotic related genes expression.

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