Relationship between SPATA18 and P53 Gene Expressions in the Sperm Cells Obtained from Normospermic and Asthenospermic Samples

Document Type : Research Article


Branch Avenue - Teachers - MANSORY martyr St.Islamic Azad University - Zanjan Branch



Introduction: Improving sperm motility results in increasing the success of a treatment cycle. Recently, sperm RNA can be used for diagnostic purposes such as whole seminal fluid and sperm analysis and sperm quality test in the patients undergoing IVF/ICSI. SPATA18-P53 pathway is considered as one of the important pathways related to sperm mitochondria, which controls mitochondrial quality through eliminating its oxidative proteins. Oxidative stress may decrease sperm motility and affect sperm quality negatively due to an increase in P53 expression. SPATA18 protein is found in the satellite fibers related to outer dense fibers in the middle piece of sperm. The downregulation of SPATA18 in asthenospermia group can represent the important function of this gene in sperm motility and fertility. The present study aimed to assess the relationship between SPATA18 and P53 gene expression in the sperm cells obtained from normospermia and asthenospermia.
Materials and method: The quantitative RT-PCR technique was used to measure the level of SPATA18 and P53 gene expression in the sperm samples collected from 21 patients and 63 healthy individuals. Further, SDFA kit was applied to determine the relative level of apoptosis in cells and evaluate the biochemical information related to the sperm samples of patients. Furthermore, all of the participants completed consent form and the study was confirmed by ethic committee.
Results: Based on the results, the level of P53 and SPATA18 gene expression in t most of the samples in which motility was less than 40% increased (p≤0.001) and decreased (p≤0.001).
Conclusion: The level of SPATA18 and P53 gene expression in asthenospermic patients increased and decreased compared to normal group, respectively. Thus, the level of P53 and SPATA18 expression can be used as an appropriate marker for diagnosing sperm motility in males.


Main Subjects