Document Type : Original Article
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Department of Clinical Studies, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Background: Cloning or SCNT (somatic cell nuclear transfer) is an approach for propagation of elite animals. In vitro condition, especially the composition of culture media has been shown to have profound effect on developmental competency of in vitro fertilized derived embryos. So far there are limited studies evaluating the effect of culture media on SCNT outcomes. To address this gap, we compare the effect of two culture media (synthetic oviduct fluid (SOF) vs. commercial Bracket-Oliphant (BO) on pre- and post-implantation development, as well as gene expression on Saanen goat SCNT embryos.
Material and method: In vitro and in vivo development of in vitro fertilized (IVF) and (SCNT) embryos were assessed in both BO and SOF media. The expression of 16 genes belonging to 7 pathways were examined at blastocyst stage.
Result: The blastocyst rate in BO medium was significantly higher than that of the SOF medium in SCNT group (P< 0.05). All of the genes examined showed increased expression levels in SCNT blastocyst in both media compared to IVF Blastocyst. In the IVF group, OCT4, BMPR1, and GCN5 showed significantly higher expression in the SOF medium compared to the BO medium while AKT, FGFR4, SOX2 showed significantly lower expression in the SOF medium compared to the BO medium. In the SCNT group, FGFR4, GCN5, FZD, CTNNB, BMPR1, and FGFR4 showed significantly higher expression in SOF compared to BO derived blastocyst.
Conclusion: it appears that in SCNT blastocysts, gene regulation is less controlled compared to IVF ones, irrespective of type of medium. In addition, there are difference regarding certain genes expressions between IVF and SCNT derived blastocysts between SOF and BO, reiterating that culture composition have an effect on developmental competency and gene expression.