Relationship between serum vitamin D in male, sperm function and clinical outcomes in infertile men candidate for ICSI

Document Type : Original Article


1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.



Background: Today, vitamin D deficiency is one of the main issues around the world, as the lack of this vitamin causes several disorders in our body, especially in organs, and cells that have vitamin D receptors.
Materials and Methods: In this observational study, we evaluated different parameters of male fertility such as, sperm parameters, oxidative stress, sperm chromatin status, sperm DNA fragmentation, as well as clinical outcomes (fertilization, embryo quality, and implantation) in sperm samples of 30 infertile couples who were candidate for ICSI. Data was then analyzed by IBM SPSS Statistics 25.0 software. We evaluated correlation between sperm parameters, stress oxidative, sperm chromatin status, sperm DNA fragmentation and serum vitamin D level. Candidates were divided in three groups according to their vitamin D level. Clinical outcomes including sperm parameters, ROS (reactive oxygen species) level, chromatin status and DNA fragmentation were compared between study groups.
Results: Analysis of all the data reveals that, there is significant correlation between vitamin D and sperm concentration (p≤0.001, r=0.5), sperm count (p=0.03, r=0.31). In addition, the result shows that there is negative significant correlation between vitamin D level and sperm ROS (p≤0.001, r= -0.77). Moreover, comparing clinical outcomes within study groups shows that there is a significant difference in implantation rate between sufficient and other groups (insufficient and deficient) (p<0.02).
Conclusion: Considering the association between sperm concentration and ROS level with vitamin D and, higher implantation rate in individuals with vitamin D sufficient group compared to other groups, our data call for vitamin D supplementation as part of male infertility treatment. But considering our sample size, further research is need to verify these findings.


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