Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Clomiphene Citrate Treatment Cycle Outcomes of Polycystic Ovary Syndrome Patients Based on Basal High Sensitive C-Reactive Protein Levels: A Cross-Sectional Study3203264539710.22074/ijfs.2016.4849ENSerkan KahyaogluDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyOmer Hamid YumuşakDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeySebnem OzyerDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyMeryem Kuru PekcanDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyMerve ErelDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyMahmut Nedim CicekDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeySalim ErkayaDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyYasemin TasciDepartment of Obstetrics and Gynecology Zekai Tahir Burak Women’s Health Education and Research Hospital Serhat Mahallesi 1292 Sokak Nevbahar Botanik 2 Konutları C Blok No 7/49 Yenimahalle Ankara TurkeyJournal Article19700101<strong>Background</strong><br /> Polycystic ovary syndrome (PCOS) is highly associated with an ovulatory infertility, features of the metabolic syndrome, including obesity, insulin resistance and dyslipidemia. Serum concentrations of high sensitive C-reactive protein (hs-CRP) were significantly higher in obese than in non-obese PCOS patients at baseline, suggesting a relationship between elevated hs-CRP levels and obesity. The aim of this study was to evaluate whether cycle day 3 hs-CRP levels before clomiphene citrate (CC) treatment would predict cycle outcomes in women with PCOS. <br /><strong>Materials and Methods</strong><br /> This cross-sectional study was conducted among 84 infertile women with PCOS who were treated with CC at Zekai Tahir Burak Women’s Health Education and Research Hospital, Ankara, Turkey, between January 2014 and January 2015. Based on the exclusion criteria, cycle outcomes of remaining 66 infertile women with PCOS treated with CC were analyzed. The hs-CRP levels and insulin resistance indexes were evaluated on day 3 of the CC treatment cycle. The primary outcome measures were number of preovulatory follicles measuring≥17 mm and pregnancy rates. <br /><strong>Results</strong><br /> The mean ± SD age of the patients was 24.0 ± 3.8 years (range 18-36). The mean ± SD body mass index (BMI) of the patients was 25.7 ± 4.9 (range 17-43). Fifty patients developed dominant follicle (75%) and 5 patients established clinical pregnancy during the study (clinical pregnancy rate: 7%). The mean ± SD baseline hs-CRP, fasting insulin and Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) values of the patients with and without dominant follicle generation during treatment cycle were 6.42 ± 7.05 and 4.41 ± 2.95 (P=0.27), 11.61 ± 6.94 and 10.95 ± 5.65 (P=0.73), 2.68 ± 1.79 and 2.41 ± 1.30 (P=0.58), respectively. The mean ± SD baseline hs-CRP, fasting insulin and HOMA-IR values of the patients with and without clinical pregnancy establishment following treatment cycle were 6.30 ± 2.56 and 5.90 ± 6.57 (P=0.89), 11.60 ± 7.54 and 11.44 ± 6.61 (P=0.95), 2.42 ± 1.51 and 2.63 ± 1.70 (P=0.79), respectively. <br /><strong>Conclusion</strong><br /> In this study, we did not observe a predictive value of cycle day 3 hs-CRP levels on preovulatory follicle development and pregnancy rates among infertile PCOS patients treated with CC. Also, no relationship between HOMA-IR values and dominant follicle generation or clinical pregnancy establishment was demonstrated in our study, confirming the previous studies emphasizing the neutral effect of metformin utilization before and/or during ovulation induction to pregnancy rates.https://www.ijfs.ir/article_45397_789ef0ed028f722442799dffd05d922d.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Molecular Detection and Genotypic Characterization of Toxoplasma gondii in Paraffin-Embedded Fetoplacental Tissues of Women with Recurrent Spontaneous Abortion3273364539810.22074/ijfs.2016.4569ENAmir AbdoliDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranAbdolhossein DalimiDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranHaleh SoltanghoraeeReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranFatemeh GhaffarifarDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranJournal Article19700101<strong>Background</strong><br /> Congenital toxoplasmosis is an important cause of spontaneous abortion worldwide. However, there is limited information on detection and genotypic characterization of Toxoplasma gondii (T. gondii) in women with recurrent spontaneous abortion (RSA). The aim of this study is the molecular detection and genotypic characterization of T. gondii in formalin-fixed, paraffin-embedded fetoplacental tissues (FFPTs) of women with RSA that have referred to the Avicenna Research Institute in Tehran, Iran. <br /><strong>Materials and Methods</strong><br /> This experimental research was undertaken on 210 FFPTs of women with RSA. The information of the patients was collected from the archives of Avicenna Research Institute in Tehran, Iran. After DNA extraction, the presence of T. gondii was examined by nested polymerase chain reaction targeting the GRA6 gene. Genotyping was performed on positive samples using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) that targeted the GRA6 and SAG3 genes. Sequencing was conducted on two GRA6 positive samples. <br /><strong>Results</strong><br /> T. gondii DNA was detected in 3.8% (8/210) of the samples. Genotyping showed that all positive samples belonged to type III of the T. gondii genotype. Sequencing two genomic DNAs of the GRA6 gene revealed 99% similarity with each other and 99-100% similarity with T. gondii sequences deposited in GenBank. There were six patients with histories of more than three abortions; one patient had a healthy girl and another patient had two previous abortions. Abortions occurred in the first trimester of pregnancy in seven patients and in the second trimester of pregnancy in one patient. <br /><strong>Conclusion</strong><br /> The results of this study have indicated that genotype III is the predominant type of T. gondii in women with RSA in Tehran, Iran. Also, our findings suggest that toxoplasmosis may play a role in the pathogenesis of RSA. However, further studies are needed to elucidate a clear relationship between T. gondii infection and RSA.https://www.ijfs.ir/article_45398_395241e090d7892e6d168ab7c48e55da.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Association between Nutritional Status with Spontaneous Abortion3373424539910.22074/ijfs.2016.4577ENRahimeh AhmadiDepartment of Midwifery and Reproductive Health, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranSaeideh ZiaeiDepartment of Midwifery and Reproductive Health, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranSosan ParsayDepartment of Midwifery and Reproductive Health, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranJournal Article19700101<strong>Background</strong><br /> Spontaneous abortion is the most common adverse pregnancy outcome. We aimed to investigate a possible link between nutrient deficiencies and the risk of spontaneous abortion. <br /><strong>Materials and Methods</strong><br /> This case-control study included the case group (n=331) experiencing a spontaneous abortion before 14 weeks of pregnancy and the control group (n=331) who were healthy pregnant women over 14 weeks of pregnancy. The participants filled out Food Frequency Questionnaire (FFQ), in which they reported their frequency of consumption for a given serving of each food item during the past three months, on a daily, weekly or monthly basis. The reported frequency for each food item was converted to a daily intake. Then, consumption of nutrients was compared between the two groups. <br /><strong>Results</strong><br /> There are significant differences between the two groups regarding consumed servings/day of vegetables, bread and cereal, meat, poultry, fish, eggs, beans, fats, oils and dairy products (P=0.012, P < 0.001, P=0.004, P < 0.001, P=0.019, respectively). There are significant differences between the two groups in all micronutrient including folic acid, iron, vitamin C, vitamin B6, vitamin B12 and zinc (P < 0.001). <br /><strong>Conclusion</strong><br /> Poor nutrientions may be correlated with increased risk of spontaneous abortion.https://www.ijfs.ir/article_45399_b5e70d5374d6df2671a3e8d33c553481.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Lack of Association between Cu T-380A Intrauterine Device and Secondary Infertility in Iran3433494540010.22074/ijfs.2016.5085ENMahnaz AbdinasabYazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranRazieh Dehghani FirouzabadiYazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranTahmineh FarajkhodaResearch Center for Nursing and Midwifery Care, Shahid Sadoughi University of Medical Sciences, Yazd, IranAli Mohammad AbdoliYazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, IranJournal Article19700101<strong>Background</strong><br /> The appropriate choice of a contraceptive method has been a major issue in reproductive health research. Cu T intrauterine device (Cu T IUD) has been introduced as one of the most effective contraceptive methods in the world, however, the relationship between prior use of Cu T IUD and secondary infertility has not been evaluated in Iran. To examine the association of Cu T-380A IUD and secondary infertility in Iran. <br /><strong>Materials and Methods</strong><br /> A retrospective cohort study was conducted from December 2010 to September 2011 in the Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. A total of 750 married women (15-49 years old) with at least one parity, whom were referred to four educational healthcare centers of Shahid Sadoughi University of Medical Sciences, were selected as participants. They were divided into two groups (case and control) based on previous history of using Cu T-380A IUD. Data were gathered using a standard reliable questionnaire along with a face-to-face interview and were analyzed with descriptive and analytical (χ²) tests. <br /><strong>Results</strong><br /> Mean period of Cu T-380A IUD usage in the case group was 57.46 ± 47.74 months and mean time length from Cu T-380A IUD removal to pregnancy was 14.87 ± 5.18 months in this group. We observed no relationship between the use of Cu T-380A IUD and frequency of secondary infertility (3.5% in the case group versus 2.7% in the control group, P=0.52). <br /><strong>Conclusion</strong><br /> Given the relatively large sample size studied here, it is unlikely that Cu T-380A IUD results in secondary infertility and may be used by Iranian women as a safe contraceptive method.https://www.ijfs.ir/article_45400_fbdae53768c86cf9a877d7c16dc5fa7e.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Chances to Have A Boy after Gender Selection by Pre-Implantation Genetic Screening Are Reduced in Couples with only Girls and without A Boy Sired by The Male Partner3503564540110.22074/ijfs.2016.4828ENSoryya PanahiDepartment of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for
Biotechnology, ACECR, Isfahan, Iran;Department of Midwifery, School of Nursing and Midwifery Care Research Center,Fariba FahamiDepartment of Midwifery, School of Nursing and Midwifery Care Research Center, Isfahan University of Medical
Sciences, Isfahan, IranMohammad Reza DeemehDepartment of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for
Biotechnology, ACECR, Isfahan, IranMarziyeh TavalaeeDepartment of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for
Biotechnology, ACECR, Isfahan, IranHamid GourabiDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, IranMohammad Hossain Nasr-EsfahaniDepartment of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for
Biotechnology, ACECR, Isfahan, Iran;4Isfahan Fertility and Infertility Center, Isfahan, Iran0000-0003-1983-3435Journal Article19700101<strong>Background</strong><br /> Gender selection and family planning have their roots in human history. Despite great interest in these fields, very few scientific propositions exist which could explain why some family do not attain the desired sex. Therefore, the aim of this study was to evaluate whether sex of previous child or children could affect the outcomes of pre-implantation genetic screening (PGS). <br /><strong>Materials and Methods</strong><br /> This historical cohort study including 218 PGS cases referring to Isfahan Fertility and Infertility Center (IFIC). Couples were grouped as those who their male child passed away or her husbands’ has a son(s) from their previous marriage (n=70) and couples who just have daughter (n=148). Male normal blastocysts were transferred for both groups. The outcomes of PGS including pregnancy, implantation and abortion rates, along with possible confounding factors were compared between the two groups. <br /><strong>Results</strong><br /> Significant differences in pregnancy, implantation and abortion rates were observed between couples whose their male partner had/has one boy (n=70) compared to those who have just girl(s) (n=148) despite similar number and quality of male normal blastocyst transferred in the two groups. Confounding factors were also considered. <br /><strong>Conclusion</strong><br /> The Ybearing spermatozoa in male partners with no history of previous boy have lower ability to support a normal development to term, compared to male partners with previous history of boy requesting family balancing.https://www.ijfs.ir/article_45401_b3b400e08952276e377232260c185d21.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Which Stage of Mouse Embryos Is More Appropriate for Vitrification?3573624540210.22074/ijfs.2016.5086ENNasibeh GhandyDepartment of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranAbbas Ali Karimpur MalekshahMolecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of
Medical Sciences, Sari, IranJournal Article19700101<strong>Background</strong><br /> Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, morula and blastocyst stage embryos and subsequent blast formation and hatching after thawing. <br /><strong>Materials and Methods</strong><br /> In this experimental study, 2-cell embryos were obtained from the oviducts of super ovulated female NMRI mice. Some embryos were randomly selected and vitrified through a two-step media protocol and cryotop. Other embryos were cultured to assess their development. During the ensuing days, some of these cultured embryos were vitrified at 4-cell, 8-cell, morula and blastocyst stages. After 10 to 14 days, the embryos were thawed to assess their survival and also cultured to determine the rate of blastocyst formation and hatching. The results were analyzed using one-way ANOVA and Tukey’s post-hoc tests. <br /><strong>Results</strong><br /> There was no significant difference in the survival rates of vitrified embryos at 2-cell, 4-cell, 8-cell, morula and blastocyst stages after thawing (P > 0.05). The blastocyst formation rate of vitrified 8-cell embryos was significantly higher than that of 2-cell embryos (P < 0.05). The hatching rate of vitrified 4-cell, 8-cell and blastocysts were significantly higher than that of 2-cell embryos (P < 0.05). <br /><strong>Conclusion</strong><br /> Vitrification is suitable for cryopreservation of all stages of mouse embryonic development. However, the best tolerance for vitrification was observed at 4and 8-cell stages of development. Accordingly, the development of vitrified embryos to blastocysts, following thawing, was most efficacious for 4 and 8-cell embryos. Compared to mouse 2-cell embryos, embryos vitrified as blastocysts had the highest rate of hatching.https://www.ijfs.ir/article_45402_72c86c7f82c3ca92caababf03b45456d.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101The Efficacy of Well-Being Therapy for Depression in Infertile Women3633704540310.22074/ijfs.2016.5087ENMajid MoeenizadehDepartment of Psychology, Faculty of Education and Psychology, Ferdowsi University of Mashhad, Mashhad, IranHaniyeh ZarifDepartment of Psychology, Faculty of Education and Psychology, Ferdowsi University of Mashhad, Mashhad, IranJournal Article19700101<strong>Background</strong><br /> Infertility is a major public health problem with physical, psychological and social dimensions. High prevalence of psychological problems has been reported in infertile women. The objective of this study was to examine the effectiveness of well- being therapy (WBT) for depression in infertile women who were referred to an infertility center in Mashhad, Iran. <br /><strong>Materials and Methods</strong><br /> This preliminary trial was conducted at the Montasariya Infertility Center, Mashhad, Iran, between July and October 2011. A group of 22 infertile women were randomly assigned into experimental (n=11) and control groups (n=11). Patients were assessed with two self-rating inventories including the Psychological Well- being (PWB) and the Depression, Anxiety and Stress Scale-21 (DASS-21) before and after the interventions and the waiting-list period. WBT was performed in 8 to 10 sessions according to the published protocol. <br /><strong>Results</strong><br /> Analysis of covariance (ANCOVA) showed a significant difference regarding the depression scores of experimental group between preand post-treatment as compared to control subjects. <br /><strong>Conclusion</strong><br /> The results suggested the feasibility and clinical advantages of adding WBT to repertoire of the treatment techniques for depression in infertile women.https://www.ijfs.ir/article_45403_475a33779aa0ebe10999c823077a01a8.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Psychometric Properties of The Fertility Quality of Life Instrument in Infertile Iranian Women3713794540410.22074/ijfs.2016.4696ENSaman MaroufizadehDepartment of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranAzadeh GhaheriDepartment of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranPayam AminiDepartment of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran0000-0001-8675-0045Reza Omani SamaniDepartment of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranJournal Article19700101<strong>Background</strong><br /> Infertility and its treatment can have a considerable effect on a person’s quality of life (QoL). The Fertility QoL (FertiQoL) questionnaire is currently the most frequently used instrument to measure QoL in people with fertility problems. This study aims to examine the reliability and validity of the FertiQoL in infertile Iranian women. <br /><strong>Materials and Methods</strong><br /> This cross-sectional study included 155 women with fertility problems in a referral fertility clinic in Tehran, Iran from January to March 2014. A battery of instruments was used: FertiQoL, Satisfaction with Life Scale (SWLS), Hospital Anxiety and Depression Scale (HADS), and a demographic questionnaire. Construct validity of the scale was evaluated using confirmatory factor analysis (CFA). We assessed internal consistency with Cronbach’s alpha and convergent validity was examined by correlating the FertiQoL with SWLS and HADS. <br /><strong>Results</strong><br /> The results of the CFA generally supported the four-factor model of Core FertiQoL and two-factor model of Treatment FertiQoL. Both FertiQoL modules and their subscales revealed acceptable internal consistency that ranged from 0.643 to 0.911. However, the FertiQoL might be improved if Q15 and T2 items were removed from the scale. These items had low loadings on the Relational and Environment factors which decreased their internal consistency. The FertiQoL and their subscales significantly correlated with both SWLS and HADS, which confirmed convergent validity. <br /><strong>Conclusion</strong><br /> The Persian version of the FertiQoL is a valid, reliable instrument to measure QoL in infertile women and seems to perform as well as the original English Version.https://www.ijfs.ir/article_45404_e9e71dd224d85ef30d0fd5786454b908.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Permissibility of Multifetal Pregnancy Reduction from The Shiite Point of View3803894540510.22074/ijfs.2016.5088ENAtefeh Zabihi BidgoliDepartment of Family Law, Faculty of Law, Imam Sadiq University, Tehran, IranFaezeh Azimzadeh ArdbiliDepartment of Theology and Law, Imam Sadiq University, Tehran, IranJournal Article19700101<strong>Background</strong><br /> Advancements in medical technology have significantly increased the possibility of successful infertility treatment. Medical interventions in the initial process of pregnancy that intend to increase the chances of pregnancy create the risk of multifetal pregnancies for both mothers and fetuses. Physicians attempt to reduce the numbers of fetuses in order to decrease this risk and guarantee the continuation of pregnancy. The aim of this paper is to understand the Shiite instruction in terms of the risks multifetal pregnancies have for fetuses and if it is permissible to reduce the numbers of fetuses. An affirmative answer will lead to the development of Islamic criteria for reduction of the number of embryos. <br /><strong>Materials and Methods</strong><br /> This analytical-descriptive research gathered relevant data as a literature search. We reviewed a number of Islamic resources that pertained to the fetus; after a description of the fundamentals and definitions, we subsequently analyzed juridical texts. The order of reduction was inevitably determined by taking into consideration the rules that governed the abortion provisions or general juridical rules. We also investigated the UK law as a comparison to the Shiite perspective. <br /><strong>Results</strong><br /> The primary ordinance states that termination of an embryo is not permitted and is considered taboo. However, fetal reductions that occur in emergency situations where there is no option or ordinary indication are permitted before the time of ensoulment. The goal of reduction can be chosen from different ways. <br /><strong>Conclusion</strong><br /> According to Shiite sources, fetal reduction is permitted. Defective fetuses are the criteria for selective reduction. If none are defective, the criteria are possibility and facility. But if the possibility of selection is equally for more than one fetus, the criterion is importance (for example one fetus is healthier).https://www.ijfs.ir/article_45405_a1a74a8eee847c868506a0b4c903d13c.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)International Journal of Fertility and Sterility2008-076X10420170101Screening of Two Neighboring CFTR Mutations in Iranian Infertile Men with Non-Obstructive Azoospermia3903944540610.22074/ijfs.2016.4593ENSomayeh HeidariDepartment of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranZohreh HojatiDepartment of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranMajid Motovali-BashiDepartment of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IranJournal Article19700101The genetic association between cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations and male infertility due to congenital bilateral absence of vas deferens (CBAVD) is well established. Mutant CFTR, however may also be involved in the etiology of male infertility in non-CBAVD cases. The present study was conducted to estimate the frequency of ∆I507 and ∆F508 CFTR gene mutations in Iranian infertile males. We undertook the first study of association between these CFTR mutations and non-obstructive azoospermia in Iran. In this case-control study, 100 fertile healthy fathers and 100 non-obstructive azoospermia’s men were recruited from Isfahan Infertility Center (IIC) and Sari Saint Mary’s Infertility Center, between 2008 and 2009. Screening of F508del and I507del mutations was carried out by the multiplex-ARMS-PCR. Significance of differences in mutation frequencies between the patient and control groups was assessed by Fisher’s exact test. The ΔF508 was detected in three patients. However there are no significant association was found between the presence of this mutated allele and infertility [OR=9.2 (allele-based) and 7.2 (individual-based), P=0.179]. None of the samples carried the ΔI507 mutation. Altogether, we show that neither ΔI507 nor ΔF508 is involved in this population of Iranian infertile males with non-obstructive azoospermia.https://www.ijfs.ir/article_45406_8b1e4f8ff14e12b14b950d176d10c149.pdf