TY - JOUR ID - 45722 TI - Combination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Absalan, Forouzan AU - Movahedin, Mansoureh AU - Mowla, Seyed Javad AD - Anatomical Sciences Department, Faculity of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Genetics Department, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2008 PY - 2008 VL - 2 IS - 3 SP - 115 EP - 120 KW - Co-culture KW - Colonization KW - Spermatogonial KW - Cryptorchidism DO - 10.22074/ijfs.2008.45722 N2 - Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. Materials and methods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (α-MEM) supplemented with 10% fetal calf serum (FCS) for three weeks. The identity of the cells was confirmed through immunocytochemistry against Oct-4 and Vimentin. Results Best results were achieved from the co-culture system spermatogonia which continued to proliferate, and eventually, type A spermatogonia colonies were found. Most of the cells in these colonies were Oct-4 positive. Conclusion Bilateral cryptorchid surgery model is a good model for enrichment of spermatogonial stem cells (SSCs). These cells can be used for molecular characterization, genetic manipulation and restoration of male fertility. UR - https://www.ijfs.ir/article_45722.html L1 - https://www.ijfs.ir/article_45722_dee1edd08a746120c38980070717a866.pdf ER -