TY - JOUR ID - 45580 TI - Temperature Decline in Embryological Culture Dishes outside Incubator JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Korakaki, Dimitra AU - Mouroutsos, Spyros AU - Tripsianis, Gregory AU - Nikolettos, Nikos AU - Asimakopoulos, Byron AD - Laboratory of Physiology, Faculty of Medicine, School of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece AD - Laboratory of Mechatronics and Systems Automation, Department of Electrical and Computer Engineering, Democritus University of Thrace, Xanthi, Greece AD - Laboratory of Medical Statistics, Faculty of Medicine, School of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece Y1 - 2020 PY - 2020 VL - 14 IS - 1 SP - 63 EP - 67 KW - Culture Dish KW - embryo culture KW - Temperature DO - 10.22074/ijfs.2020.5917 N2 - Background In embryological culture dishes, there is a temperature decline when they are removed outside incubators. This study aimed at investigating the effects of this temperature decline within a certain time frame, the type of culture dish with or without the use of laminar air flow and whether it is possible to achieve a sufficient thermal control with the use of a heating stage. Materials and Methods In this experimental study, the temperatures of four different types of polystyrene dishes [50 mm intracytoplasmic sperm injection (ICSI), 35 mm, 60 mm, 90 mm], filled with culture medium and oil were recorded for a period of 10 minutes outside the incubator. Temperature was measured with an infrared thermographic camera. The reference temperature was 37°C. Four parameters were analyzed: the type of dishes, air flow, a heating stage at 37°C and 38.5°C. Results There was a time-dependant significant temperature decline outside the incubator in all types of dishes and under all experimental conditions. Under air flow temperature decline increased compared to the no air flow condition. The use of a heating stage at either 37°C or 38.5°C slightly improved the situation in most cases. After three minutes out of the incubator without a heating stage and air flow, the temperature was UR - https://www.ijfs.ir/article_45580.html L1 - https://www.ijfs.ir/article_45580_95692e9df1538e4035c74bc55e608251.pdf ER -