TY - JOUR ID - 45428 TI - Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Ramgir, Shalaka S AU - Sekar, Nishu AU - Jindam., Divya AU - V.G., Abilash AD - Department of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu- 632014, India Y1 - 2017 PY - 2017 VL - 11 IS - 3 SP - 142 EP - 147 KW - CYP1A1 KW - Restriction Fragment Length Polymorphism KW - infertility KW - Cohort DO - 10.22074/ijfs.2017.4752 N2 - Background: Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world. The aim of this study was to investigate the association of CYP1A1 polymorphisms with idiopathic non-obstructive azoospermia in a South Indian cohort. Materials and Methods: An experimental study was conducted with idiopathic nonobstructive azoospermia. A total of 120 infertile and 80 fertile samples were collected, and DNA was then extracted from all samples. The CYP1A1*2A polymorphism genotyping was carried out by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: The genotype distribution of CYP1A1*2A polymorphism showed significant difference between patients and controls. Moreover, the CC genotype was associated with decreased risk of idiopathic non-obstructive azoospermia in comparison with the TT and TC genotypes. Conclusion: The current experimental study identified that the CT genotype of CYP1A1*2A polymorphism may contribute to the pathogenesis of male infertility in the South Indian population. UR - https://www.ijfs.ir/article_45428.html L1 - https://www.ijfs.ir/article_45428_dd1afdbb8d1da1f13aabc6d0afcf7564.pdf ER -