TY - JOUR ID - 45422 TI - Evaluation of Relationship between Body Mass Index with Vitamin D Receptor Gene Expression and Vitamin D Levels of Follicular Fluid in Overweight Patients with Polycystic Ovary Syndrome JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Aghadavod, Esmat AU - Mollaei, Hakimeh AU - Nouri, Mohammad AU - Hamishehkar, Hamed AD - Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran AD - Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Biology, Science and Research Branch of Islamic Azad University, Tehran, Iran AD - 4Women Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - 5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2017 PY - 2017 VL - 11 IS - 2 SP - 105 EP - 111 KW - Polycystic Ovary Syndrome KW - Vitamin D KW - Granulosa cells KW - follicular fluid DO - 10.22074/ijfs.2017.4704 N2 - Background Polycystic ovary syndrome (PCOS) is the most common endocrine disorder associated with reproductive disorders and metabolic dysfunctions including insulin resistance. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The present study is to evaluate the effect of body mass index (BMI) on vitamin D levels in follicular fluid and vitamin D receptor (VDR) expression levels in granulos cells. Materials and Methods A comparative study was conducted on 80 women with average age of 20-35 years referred for in vitro fertilization (IVF). Patients were divided into four groups, and serum levels of testosterone and insulin resistance (IR) were evaluated at the puncture time. Also, vitamin D levels of follicular fluid were evaluated. VDR gene expression was assayed by quantified-polymerase chain reaction (PCR) technique. Correlations were evaluated with calculation of the Spearman coefficient, and also independent relationships were assessed by means of multiple regression analysis. Results Vitamin D levels of follicular fluid decreased in PCOS patients compared with non-PCOS. Also, over-weight individuals had lower vitamin D levels compared with normal-weight patients. Vitamin D levels of follicular fluid were highly correlated with BMI (r=-0.51, P < 0.01). Homeostatic model assessment-IR (HOMA-IR) values were significantly higher in women of PCOS/overweight and PCOS/normal weight in comparison with women of non-PCOS/normal weight (P < 0.01). The gene expression data of VDR in granulosa cells were significantly lower in the PCOS/overweight group compared with the non-PCOS/normal weight (P < 0.01). Conclusion The findings indicated significant differences in VDR gene expression in granulosa cells and vitamin D of follicular fluid in PCOS/overweight patients. UR - https://www.ijfs.ir/article_45422.html L1 - https://www.ijfs.ir/article_45422_e4baceb20062cbd923ae9e713332a870.pdf ER -