TY - JOUR ID - 45350 TI - The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Bazrgar, Masood AU - Gourabi, Hamid AU - Eftekhari-Yazdi, Poopak AU - Vazirinasab, Hamed AU - Fakhri, Mostafa AU - Hassani, Fatemeh AU - Chehrazi, Mohamad AU - Valojerdi, Mojtaba Rezazadeh AD - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;Department of Developmental Biology, University of Science and Culture, Tehran, Iran AD - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran AD - Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran AD - 4Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran Y1 - 2016 PY - 2016 VL - 9 IS - 4 SP - 563 EP - 573 KW - Aneuploidy KW - Blastocyst KW - Self KW - correction KW - Mosaicism DO - 10.22074/ijfs.2016.4617 N2 - Background A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. Materials and Methods In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. Results Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P < 0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. Conclusion Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells. UR - https://www.ijfs.ir/article_45350.html L1 - https://www.ijfs.ir/article_45350_c0a259beb9c45d075c868ddae0204763.pdf ER -