TY - JOUR ID - 45219 TI - Chromosomal Abnormality in Men with Impaired Spermatogenesis JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Mierla, Dana AU - Jardan, Dumitru AU - Stoian, Veronica AD - Life Memorial Hospital, Bucharest, Romania;Department of Genetics, Faculty of Biology, University of Bucharest, Bucharest, Romania AD - Life Memorial Hospital, Bucharest, Romania AD - Department of Genetics, Faculty of Biology, University of Bucharest, Bucharest, Romania Y1 - 2014 PY - 2014 VL - 8 IS - 1 SP - 35 EP - 42 KW - Chromosomal abnormality KW - Chromosome Microdeletion KW - Male infertility KW - Azoospermia KW - Oligozoospermia DO - N2 - Background: Chromosomal abnormalities and Y chromosome microdeletions are regarded as two most frequent genetic causes associated with failure of spermatogenesis in the Caucasian population. Materials and Methods: To investigate the distribution of genetic defects in the Romanian population with azoospermia or severe oligozoospermia, karyotype analysis by G-banding was carried out in 850 idiopathic infertile men and in 49 fertile men with one or more children. Screening for microdeletions in the azoospermia factor (AZF) region of Y chromosome was performed by multiplex polymerase chain reaction (PCR) on a group of 67 patients with no detectable chromosomal abnormality. The results of the two groups were compared by a two-tailed Fisher’s exact test. Results: In our study chromosomal abnormalities were observed in 12.70% and 8.16% of infertile and fertile individuals respectively. Conclusion: Our data suggests that infertile men with severe azoospermia have higher incidences of genetic defects than fertile men and also patients from any other group. Infertile men with normal sperm present a higher rate of polymorphic variants. It is important to know whether there is a genetic cause of male infertility before patients are subjected to intracytoplasmic sperm injection (ICSI) or testicular sperm extraction (TESE)/ICSI treatment. UR - https://www.ijfs.ir/article_45219.html L1 - https://www.ijfs.ir/article_45219_8c00af110eda6ef51c0a28ac2019bdeb.pdf ER -