TY - JOUR ID - 247970 TI - Therapeutic Effects of Eugenol in Polycystic Ovarian Rats Induced by Estradiol Valerate: A Histopathological and A Biochemical Study JO - International Journal of Fertility and Sterility JA - IJFS LA - en SN - 2008-076X AU - Kokabiyan, Zahra AU - Yaghmaei, Parichehreh AU - Jameie, Seyed Behnamedin AU - Hajebrahimi, Zahra AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Department of Anatomy, Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran AD - A&S Research Institute, Ministry of Science Research and Technology, Tehran, Iran Y1 - 2022 PY - 2022 VL - 16 IS - 3 SP - 184 EP - 191 KW - COX-2 KW - Eugenol KW - metabolic dysfunction KW - Polycystic Ovary Syndrome KW - Ppar-α DO - 10.22074/ijfs.2021.537724.1176 N2 - Background: Polycystic ovary syndrome (PCOS) is a common type of endocrinopathy in women which is accompanied by androgens elevation, insulin resistance, and metabolic dysfunction. Eugenol is a phenolic component of clove oil that has an antioxidant, anti-inflammatory, and anti-diabetic activity. The present study aimed to evaluate the therapeutic effects of eugenol on the PCOS models of rats. Materials and Methods: In this experimental study, thirty adults female Wistar rats weighing between 180 and 200 g were used. Estradiol valerate-induced PCOS rats (4 mg/rat) were treated with eugenol (12 and 24 mg/kg) for 28 days. The effects of eugenol were studied on levels of glucose, lipid profile, liver enzymes, reproductive hormones, oxidative stress, and the expression of cyclooxygenase-2 (Cox-2) and peroxisome proliferator-activated receptor alpha (Ppar-α) genes, using biochemical analysis of blood and histopathological evaluation of ovaries.Results: Estradiol valerate-induced PCOS resulted in the formation of cystic follicles in the ovaries, hyperinsulinemia, hyperglycemia, hyperlipidemia, hyperandrogenism, and anovulation. It altered the Cox-2 and Ppar-α gene expression and increased oxidative stress and activities of liver enzymes. Eugenol treatment improved the PCOS-associated endocrine and metabolic disorder and histopathological alterations, mostly through antioxidant, anti-diabetic, anti hyperlipidemic, and anti-androgenic properties. It showed beneficial effects on serum glucose, serum insulin, fat profile, reproductive hormones, liver activity, oxidative stress, expression of Cox-2 and Ppar-α genes, as well as restoration of normal ovulation in the PCOS animals.Conclusion: Eugenol could represent a promising natural product to prevent PCOS or reduce its symptoms. UR - https://www.ijfs.ir/article_247970.html L1 - https://www.ijfs.ir/article_247970_faebc7a04993d97408bd9625e6f3bc19.pdf ER -