Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Reactive Oxygen Species Generation and Use of Antioxidants during In Vitro Maturation of Oocytes
63
70
EN
Mozafar
Khazaei
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Faranak
Aghaz
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
faranak_aghaz@yahoo.com
10.22074/ijfs.2017.4995
In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture (IVC) conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic development. It is well-known that higher than physiological levels of ROS trigger granulosa cell apoptosis and thereby reduce the transfer of nutrients and survival factors to oocytes, which leads to apoptosis. ROS are neutralized by an elaborate defense system that consists of enzymatic and non-enzymatic antioxidants. The balance between ROS levels and antioxidants within IVM media are important for maintenance of oocytes that develop to the blastocyst stage. The effects of antioxidant supplementation of IVM media have been studied in various mammalian species. Therefore, this article reviews and summarizes the effects of ROS on oocyte quality and the use of antioxidant supplementations for IVM, in addition to its effects on maturation rates and further embryo development.
Oxidative stress,Reactive Oxygen Species,Antioxidant,maturation
https://www.ijfs.ir/article_45416.html
https://www.ijfs.ir/article_45416_0764e820ea499d2f0a726b0341e76b15.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Hysterosalpingography in The Assessment of Congenital Cervical Anomalies
71
78
EN
Fatemeh
Zafarani
Department of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Tehran, Iran
Firoozeh
Ahmadi
Department of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Tehran, Iran
f_ahmadi@royaninstitute.org
Gholam
Shahrzad
Department of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Tehran, Iran
dr.gh.shahrzad@gmail.com
10.22074/ijfs.2017.4716
Cervical abnormalities may be congenital or acquired. Congenital anomalies of the cervix are rarely isolated, and more commonly accompany other uterine anomalies. Various imaging tools have been used in the assessment of Müllerian duct anomalies (MDAs). Currently, magnetic resonance imaging (MRI) is the modality of choice for definitive diagnosis and classification of these MDAs. Hysterosalpingography is a basic tool for evaluation of infertility and allows us to detect a spectrum of anatomical malformations of the utero-cervix in the setting of MDAs. It provides good outlines of the uterine cavity and fallopian tubes, as well as the cervical canal and isthmus. However, hysterosalpingograms (HSG) cannot be performed in patients with isolated congenital maldevelopment (agenesis/disgenesis) of the cervix. This part of pictorial review illustrates the various radiographic appearances of congenital malformations of the utero-cervix with a brief overview of the embryologic features. Accurate diagnosis of such cases is considered essential for optimal treatment and categorization of each anomaly.
Hysterosalpingography,Congenital,Cervix,Uterine,Anomalies
https://www.ijfs.ir/article_45417.html
https://www.ijfs.ir/article_45417_f2d502daf08f04e6882699e5557d1899.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
A Comparison of Outcomes from In Vitro Fertilization Cycles Stimulated with Follicle Stimulating Hormone Plus either Recombinant Luteinizing Hormone or Human Menopausal Gonadotropins in Subjects Treated with Long Gonadotropin Releasing Hormone Agonist Protocols
79
84
EN
Nathalie
Bleau
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
nathalie.bleau@mail.mcgill.ca
Mohammed
Agdi
Department of Reproductive Endocrinology and Infertility, McGill Reproductive Center, McGill University,
Montreal, Quebec, Canada
mohammedagdi@gmail.com
WeonYoung
Son
Department of Reproductive Endocrinology and Infertility, McGill Reproductive Center, McGill University,
Montreal, Quebec, Canada
SeangLin
Tan
Department of Reproductive Endocrinology and Infertility, McGill Reproductive Center, McGill University,
Montreal, Quebec, Canada
seanglin.tan@muhc.mcgill.ca
Michael H
Dahan
0000-0002-8121-7708
Department of Reproductive Endocrinology and Infertility, McGill Reproductive Center, McGill University,
Montreal, Quebec, Canada
dahanhaim@hotmail.com
10.22074/ijfs.2017.4759
<b>Background</b><br> This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects stimulated with follicle stimulating hormone (FSH) plus either recombinant human luteinizing hormone (r-LH) or human menopausal gonadotropin (hMG) in a long gonadotropin releasing hormone (GnRH) agonist IVF protocol. <br><b>Materials and Methods</b><br> This cohort study enrolled patients who underwent IVF stimu- lation with a long GnRH agonist protocol and received FSH plus r-LH or hMG. Outcomes measured included: FSH and LH doses, number of oocytes and embryos obtained, pregnancy rate per cycle, and clinical pregnancy rate per cycle. Stepwise logistic regression was performed on continuous and categorical variables to control for confounding effects between all variables analyzed. <br><b>Results</b><br> There were 122 patients who underwent 122 IVF cycles with long GnRH agonist protocols. Similar baseline parameters existed between groups. Patients that received r-LH required a lower FSH dose (3207 ± 1300 IU) for stimulation compared to the group that received hMG (4213 ± 1576 IU, P=0.0001). The LH dose was also lower in these patients (1332 ± 587 IU) compared to the patients who received hMG (1938 ± 1110 IU, P=0.0001). The number of days of stimulation did not differ between groups (P=1.0). The group that received r-LH also had statistically higher numbers of oocytes (14.4 ± 6.3) and embryos (7.9 ± 4.8) compared to the hMG group with 11.0 ± 5.3 oocytes and 6.0 ± 3.7 embryos. Pregnancy rates per cycle start were higher for patients in the r-LH group (49%) compared to the hMG group (27%, P=0.025). Patients that received r-LH had higher implantation rates (62%) compared to the hMG group (33%, P=0.001). The r-LH group had a higher trend toward clinical pregnancy rates per cycle start (39%) compared to the hMG group (25%, P=0.065). <br><b>Conclusion</b><br> r-LH may offer benefits compared to hMG when combined with FSH for ovarian stimulation in long GnRH agonist protocols in good responders. Prospective studies should be undertaken to confirm these results.
Luteinizing hormone,Fertilization,human menopausal gonadotropins,ovarian stimulation
https://www.ijfs.ir/article_45418.html
https://www.ijfs.ir/article_45418_98144040662d6d19d3264265fc4d08cc.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Vitrification of Human Germinal Vesicle Oocytes: before or after In Vitro Maturation?
85
92
EN
Evangelia
Kasapi
Iakentro Fertility Centre, IVF Laboratory, Thessaloniki, Greece;Democritus University of Thrace, Laboratory of Physiology, Faculty of Medicine, School of Health Sciences,
Alexandroupolis, Greece
lia@iakentro.gr
Byron
Asimakopoulos
Democritus University of Thrace, Laboratory of Physiology, Faculty of Medicine, School of Health Sciences,
Alexandroupolis, Greece
basima@med.duth.gr
Katerina
Chatzimeletiou
Aristotle University of Thessaloniki, Medical School, 1st
Department of Obstetrics and Gynaecology,
Papageorgiou General Hospital, Thessaloniki, Greece
Stamatios
Petousis
Iakentro Fertility Centre, IVF Laboratory, Thessaloniki, Greece
petustam@mail.gr
Yannis
Panagiotidis
Iakentro Fertility Centre, IVF Laboratory, Thessaloniki, Greece
panagy@iakentro.gr
Nikos
Prapas
Iakentro Fertility Centre, IVF Laboratory, Thessaloniki, Greece
nikos@iakentro.gr
Nikos
Nikolettos
4Embryokosmogenesis, IVF Laboratory, Alexandroupolis, Greece
nnikolettos@gmail.com
10.22074/ijfs.2017.4717
<b>Background</b><br> The use of immature oocytes derived from stimulated cycles could be of great importance, particularly for urgent fertility preservation cases. The current study aimed to determine whether in vitro maturation (IVM) was more successful before or after vitrification of these oocytes. <br><b>Materials and Methods</b><br> This prospective study was performed in a private in vitro fertilization (IVF) center. We collected 318 germinal vesicle (GV) oocytes from 104 stimulated oocyte donation cycles. Oocytes were divided into two groups according to whether vitrification was applied at the GV stage (group 1) or in vitro matured to the metaphase II (MII) stage and then vitrified (group 2). In the control group (group 3), oocytes were in vitro matured without vitrification. In all three groups, we assessed survival rate after warming, maturation rate, and MII-spindle/chromosome configurations. The chi-square test was used to compare rates between the three groups. Statistical significance was defined at P < 0.05 and we used Bonferroni criterion to assess statistical significance regarding the various pairs of groups. The Statistical Package for the Social Sciences version 17.0 was used to perform statistical analysis. <br><b>Results</b><br> There was no significant difference in the survival rate after vitrification and warming of GV (93.5%) and MII oocytes (90.8%). A significantly higher maturation rate occurred when IVM was performed before vitrification (82.9%) compared to after vitrification (51%). There was no significant difference in the incidence of normal spindle/ chromosome configurations among warmed oocytes matured in vitro before (50.0%) or after (41.2%) vitrification. However, a higher incidence of normal spindle/chromosome configurations existed in the in vitro matured oocytes which were not subjected to vitrification (fresh oocytes, 77.9%). <br><b>Conclusion</b><br> In stimulated cycles, vitrification of in vitro matured MII oocytes rather than GV oocytes seems to be more efficient. This approach needs to be verified in nonstimulated fertility preservation cases.
Vitrification,maturation,meiotic spindle,fertility preservation
https://www.ijfs.ir/article_45419.html
https://www.ijfs.ir/article_45419_aaedd7d752decf268ee8f679adf6fd5f.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Comparative Stepwise Pattern of Reactive Oxygen Species Production during In Vitro Development of Fertilized and Nuclear Transferred Goat Embryos
93
98
EN
Mehdi
Hajian
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center,
Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Sayed Morteza
Hosseini
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center,
Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Somayyeh
Ostadhosseini
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center,
Royan Institute for Biotechnology, ACECR, Isfahan, Iran
somayyehostadhossaini@gmail.com
Mohammad Hossein
Nasr-Esfahani
0000-0003-1983-3435
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center,
Royan Institute for Biotechnology, ACECR, Isfahan, Iran;Department of Embryology, Reproductive Biomedicine Research Center,
mh.nasr-esfahani@royaninstitute.org
10.22074/ijfs.2017.5049
<b>Background</b><br> A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somatic cell nuclear transfer (SCNT). Furthermore, the pattern of ROS production of SCNT embryos were compared with zona free embryos derived from in vitro fertilization (IVF). <br><b>Materials and Methods</b><br> In this experimental study, zona-free oocytes, SCNT and IVF embryos at different stages of in vitro development (2, 4, 8, 16-cells, morula, and blastocyst) were used for assessment of ROS production using 2, 7-dichloro dihydroflourescein diacetate (DCHFDA) probe and the result were presented as fold increase or decrease relative zona free oocytes. <br><b>Results</b><br> The relative level of ROS compared to metaphase-II (MII) oocytes insignificantly decrease during early stages post embryo reconstitution and regained its value by 8-cell and morula stage and, significantly increase compared to MII oocytes by blastocyst stage. <br><b>Conclusion</b><br> The pattern of ROS change in SCNT embryos is similar to zona free IVF derived embryos, except it decrease from two cell stage and regain its value at morula stage. The sudden rise in ROS at blastocyst stage, further emphasizes the special need of IVF and SCNT derived embryos during this stage of development.
somatic cell nuclear transfer,Reactive Oxygen Species,Goat
https://www.ijfs.ir/article_45420.html
https://www.ijfs.ir/article_45420_66f781326953b477eb3d4583f888ab95.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Kisspeptin: A Potential Factor for Unexplained Infertility and Impaired Embryo Implantation
99
104
EN
Aaida
Mumtaz
Medical College, Aga Khan University, Karachi, Pakistan
aaidamumtaz@msn.com
Aqsa
Khalid
Medical College, Aga Khan University, Karachi, Pakistan
aqsakhalid13@hotmail.com
Zehra
Jamil
Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
zehra.jamil@aku.edu
Syeda Sadia
Fatima
Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
Sara
Arif
Civil Hospital, Karachi, Pakistan
Rehana
Rehman
Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
rehana.rehman@aku.edu
10.22074/ijfs.2017.4957
<b>Background</b><br> Kisspeptin (KP) is a neuropeptide that causes the release of the gonadotropin releasing hormone, which controls hypothalamo pituitary ovarian axis and exerts a number of peripheral effects on reproductive organs. The primary objective of this study was to compare baseline KP levels in females with different types of infertility and identify possible correlations with risk of failure to conceive, preclinical abortion and pregnancy after intracytoplasmic sperm injection (ICSI). <br><b>Materials and Methods</b><br> A longitudinal cohort study was carried out from August 2014 until May 2015 by recruiting 124 female patients undergoing ICSI, after obtaining ethical approval from the Australian Concept Infertility Medical Center. Cause of infertility due to male, female and unexplained factors was at a frequency of 32 (24%), 33 (31%) and 59 (45%) among the individuals respectively. KP levels were measured by ELISA assay before the initiation of the ICSI treatment protocol. Outcome of ICSI was categorized into three groups of non-pregnant with beta-human chorionic gonadotropin (β-hCG)25 mIU/ml and no cardiac activity, and clinical pregnancy declared upon confirmation of cardiac activity. Results based on cause of infertility and outcome groups were analyzed by one-way ANOVA. <br><b>Results</b><br> Females with unexplained infertility had significantly lower levels of KP when compared with those with male factor infertility (176.69 ± 5.03 vs. 397.6 ± 58.2, P=0.001). Clinical pregnancy was observed in 28 (23%) females of which 17 (71%) had a female cause of infertility. In the non-pregnant group of 66 (53%) females, common cause of infertility was unexplained 56(85%). A weak positive correlation of KP levels with fertilized oocytes and endometrial thickness was observed (P=0.04 and 0.01 respectively). <br><b>Conclusion</b><br> Deficiency of KP in females with unexplained infertility was associated with reduced chances of implantation after ICSI.
infertility,ovarian stimulation,Intracytoplasmic Sperm Injection,Kisspeptin
https://www.ijfs.ir/article_45421.html
https://www.ijfs.ir/article_45421_a0603a301db737072f3693b89974c8b4.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Evaluation of Relationship between Body Mass Index with Vitamin D Receptor Gene Expression and Vitamin D Levels of Follicular Fluid in Overweight Patients with Polycystic Ovary Syndrome
105
111
EN
Esmat
Aghadavod
Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
aghadavodm@gmail.com
Hakimeh
Mollaei
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Biology, Science and Research Branch of Islamic Azad University, Tehran, Iran
Mohammad
Nouri
4Women Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
nourim@tbzmed.ac.ir
Hamed
Hamishehkar
5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
hamishehkarh@tbzmed.ac.ir
10.22074/ijfs.2017.4704
<b>Background</b><br> Polycystic ovary syndrome (PCOS) is the most common endocrine disorder associated with reproductive disorders and metabolic dysfunctions including insulin resistance. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The present study is to evaluate the effect of body mass index (BMI) on vitamin D levels in follicular fluid and vitamin D receptor (VDR) expression levels in granulos cells. <br><b>Materials and Methods</b><br> A comparative study was conducted on 80 women with average age of 20-35 years referred for in vitro fertilization (IVF). Patients were divided into four groups, and serum levels of testosterone and insulin resistance (IR) were evaluated at the puncture time. Also, vitamin D levels of follicular fluid were evaluated. VDR gene expression was assayed by quantified-polymerase chain reaction (PCR) technique. Correlations were evaluated with calculation of the Spearman coefficient, and also independent relationships were assessed by means of multiple regression analysis. <br><b>Results</b><br> Vitamin D levels of follicular fluid decreased in PCOS patients compared with non-PCOS. Also, over-weight individuals had lower vitamin D levels compared with normal-weight patients. Vitamin D levels of follicular fluid were highly correlated with BMI (r=-0.51, P < 0.01). Homeostatic model assessment-IR (HOMA-IR) values were significantly higher in women of PCOS/overweight and PCOS/normal weight in comparison with women of non-PCOS/normal weight (P < 0.01). The gene expression data of VDR in granulosa cells were significantly lower in the PCOS/overweight group compared with the non-PCOS/normal weight (P < 0.01). <br><b>Conclusion</b><br> The findings indicated significant differences in VDR gene expression in granulosa cells and vitamin D of follicular fluid in PCOS/overweight patients.
Polycystic Ovary Syndrome,Vitamin D,Granulosa cells,follicular fluid
https://www.ijfs.ir/article_45422.html
https://www.ijfs.ir/article_45422_e4baceb20062cbd923ae9e713332a870.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
112
116
EN
Maryam
Khademi Bami
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran
khademi6586@gmail.com
Masoud
Dehghan Tezerjani
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
masoud.msdco@gmail.com
Fateme
Montazeri
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
marjan.montazeri@gmail.com
Hamid Reza
Ashrafzadeh Mehrjardi
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
hr_ashrafzadeh@yahoo.com
Saeed
Ghasemi-Esmailabad
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
qasemisaeed@yahoo.com
Mohammad Hasan
Sheikhha
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Seyed Mehdi
Kalantar
Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
smkalantar@yahoo.com
10.22074/ijfs.2017.4830
<b>Background</b><br> Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and sperm apoptosis. Tumor necrosis factor alpha (TNFα) is one of the most-documented cytokines that is involved in spermatogenesis. We investigated the association of the TNFα -308 G/A single nucleotide polymorphism with sperm abnormalities in Iranian males. <br><b>Materials and Methods</b><br> This case-control study included 180 infertile men who re- ferred to Yazd Research and Clinical Center for Infertility and 100 healthy normospermic controls. Infertile men were classified into four groups of azoospermia (n=91), oligospermia (n=26), teratospermia (n=30) and asthenoteratospermia (n=33). After sperm analysis, DNA was extracted from blood and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for the genotyping of TNFα- 308 G/A. <br><b>Results</b><br> The A allele was significantly associated with sperm abnormality in our population [(P < 0.001, odds ratios (OR) 95% confidence interval (CI)=2.31]. In addition, the A allele was also associated with azoospermia (P < 0.001, OR (95% CI)=2.484), oligospermia (P=0.005, OR (95% CI)=2.51) and teratospemia (P < 0.001, OR (95% CI)=3.385) but not with asthenoteratospermia (P=0.623). <br><b>Conclusion</b><br> Our data suggest that this single nucleotide polymorphism (SNP) maybe associated with the risk of sperm abnormality in infertile men of Iranian origin.
infertility,Cytokines,Tumor Necrosis Factor Alpha,Polymorphism,Restriction Fragment Length Polymorphism
https://www.ijfs.ir/article_45423.html
https://www.ijfs.ir/article_45423_85b7be56dbb7647de877953ffea400b5.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Sperm Retrieval in Patients with Klinefelter Syndrome: A Skewed Regression Model Analysis
117
122
EN
Mohammad
Chehrazi
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran;Department of Epidemiology and Biostatistics, School of Pu
Abbas
Rahimiforoushani
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
rahimifo@tums.ac.ir
Keramat
Nourijelyani
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
nourik@tums.ac.ir
Mohammad Ali
Sadighi Gilani
Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, Iran;4Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehr
ali.sadighi@royaninstitute.org
Mostafa
Hoseini
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Samira
Vesali
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
samiravesali@yahoo.com
Mehdi
Yaseri
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
m.yaseri@gmail.com
Ahad
Alizadeh
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
st.alizadeh@gmail.com
Kazem
Mohammad
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
mohamadk@tums.ac.ir
Reza Omani
Samani
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
10.22074/ijfs.2017.4702
<b>Background</b><br> The most common chromosomal abnormality due to non-obstructive azoospermia (NOA) is Klinefelter syndrome (KS) which occurs in 1-1.72 out of 500-1000 male infants. The probability of retrieving sperm as the outcome could be asymmetrically different between patients with and without KS, therefore logistic regression analysis is not a well-qualified test for this type of data. This study has been designed to evaluate skewed regression model analysis for data collected from microsurgical testicular sperm extraction (micro-TESE) among azoospermic patients with and without non-mosaic KS syndrome. <br><b>Materials and Methods</b><br> This cohort study compared the micro-TESE outcome between 134 men with classic KS and 537 men with NOA and normal karyotype who were referred to Royan Institute between 2009 and 2011. In addition to our main outcome, which was sperm retrieval, we also used logistic and skewed regression analyses to compare the following demographic and hormonal factors: age, level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone between the two groups. <br><b>Results</b><br> A comparison of the micro-TESE between the KS and control groups showed a success rate of 28.4% (38/134) for the KS group and 22.2% (119/537) for the control group. In the KS group, a significantly difference (P < 0.001) existed between testosterone levels for the successful sperm retrieval group (3.4 ± 0.48 mg/mL) compared to the unsuccessful sperm retrieval group (2.33 ± 0.23 mg/mL). The index for quasi Akaike information criterion (QAIC) had a goodness of fit of 74 for the skewed model which was lower than logistic regression (QAIC=85). <br><b>Conclusion</b><br> According to the results, skewed regression is more efficient in estimating sperm retrieval success when the data from patients with KS are analyzed. This finding should be investigated by conducting additional studies with different data structures.
Klinefelter Syndrome,Sperm Retrieval,Logistic regression
https://www.ijfs.ir/article_45424.html
https://www.ijfs.ir/article_45424_8ac9d73cb54db8b4ce3047b117825409.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Evaluation on Hope and Psychological Symptoms in Infertile Couples Undergoing Assisted Reproduction Treatment
123
129
EN
Reza
Omani Samani
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
info@royaninstitet.org
Samira
Vesali
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
samiravesali@yahoo.com
Behnaz
Navid
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
behnaz_navid@yahoo.com
Bahareh
Vakiliniya
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Maryam
Mohammadi
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
maryammohammadi8766@gmail.com
10.22074/ijfs.2017.4838
Background: This study evaluated hope, depression, anxiety, and stress among three groups of infertile couples. Materials and Methods: This cross-sectional study consisted of three groups of infertile couples-candidates for oocyte donation (n=60), embryo donation (n=60), and normal infertile (n=60). Participants included couples seen at Royan Institute, Tehran, Iran between 2013-2014 who were at least 18 years of age and could read and write in Persian. Participants provided demographic and general characteristics and completed the Persian version of the Adult Trait Hope Scale (hope, agency and pathway) and Depression, Anxiety, and Stress Scale (DASS). Data was analyzed by the paired t test, ANOVA, ANCOVA and Pearson correlation tests using SPSS statistical software. Results: Overall, 180 infertile couples participated in the three groups. There was a significant higher mean score for hope in husbands compared to wives in the normal infertile group (P=0.046). Husbands in the normal infertile group also had a significantly higher mean score for pathway (P=0.032). The frequency of anxiety significantly differed in female subjects (P=0.028). In the normal infertile group, the anxiety distribution significantly differed between wives and husbands (P=0.006). There was a significantly different stress frequency in male subjects (P=0.048). In the embryo donation group, stress significantly differed between wives and husbands (P=0.002). In the normal infertile group, stress also significantly differed between wives and husbands (P=0.05). Conclusion: The results have suggested that hope might be important in reducing psychological symptoms and psychological adjustment in those exposed to infertility problems who follow medical recommendations, which accelerates recovery. It is recommended to hold psychological counseling sessions (hope therapy) during reproduction cycles.
hope,Depression,Anxiety,Stress,infertility
https://www.ijfs.ir/article_45425.html
https://www.ijfs.ir/article_45425_e3600fd1638b8cc2019dafa60366941e.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
2
2017
07
01
Relationship between Chlamydia-Ureaplasma-Mycoplasma Genital Detection with Semen Concentration and Motility among Greek Men
130
133
EN
Ageliki
Gerovassili
Biogonidiaki, Center of Infertility Investigation and Genetic Research, Volos, Greece
agerovassili@googlemail.com
Ourania
Marcandona
Biogonidiaki, Center of Infertility Investigation and Genetic Research, Volos, Greece
ranja@hol.gr
Byron
Asimakopoulos
Democritus University of Thrace, Laboratory of Physiology, Alexandroupolis, Greece
Vasilis
Karavasilis
IASO General Hospital, Department of Microbiology, Larissa, Greece
karavassilis_v@iaso.gr
Maria
Panopoulou
4Democritus University of Thrace, Department of Microbiology, Alexandroupolis, Greece
mpanopou@med.duth.gr
Alexandros
Ikonomidis
Biogonidiaki, Center of Infertility Investigation and Genetic Research, Volos, Greece;4Democritus University of Thrace, Department of Microbiology, Alexandroupolis, Greece
info@biogonidiaki.gr
10.22074/ijfs.2017.4690
One hundred and seventy two men at the State of Thessaly, Greece, inquiring semen analysis were enrolled in the study in order to investigate the incidence of Chlamydia, Ureaplasma and Mycoplasma (C-U-M) genera in respect to total sperm number (TSN), progressive motility (grades a and b) and total motility (grades a, b and c). Putative relation of C-U-M acquirement with sexual behavior was also investigated. Incidence of C-U-M among non-oligozoospermic and oligozoospermic men was similar. Νο correlation of C-U-M carriage to either oligozoospermia or asthenozoospermia was found. The tested semen parameters were negatively correlated to the age of sexual intercourse initiation and positively correlated to the number of sex partners. Early age of sexual intercourse initiation or high number of sexual partners was not statistical significantly correlated to C-U-M acquirement. Overall, TSN and motility (either progressive or total) were not influenced by the presence of C-U-M genera in a sample of Greek population undergoing semen evaluation. To distinguish the role of C-U-M in male infertility and clarify the so far controversial scarce literature, large control case studies are needed using nucleic acid amplification techniques to detect these pathogens.
Polymerase chain reaction,Oligozoospermia,asthenozoospermia,Azoospermia,infertility
https://www.ijfs.ir/article_45426.html
https://www.ijfs.ir/article_45426_9f573c7f9c3639cf1b0186ab2349b8f5.pdf