Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
The Effect of Coasting on Intracytoplasmic Sperm Injection Outcome in Antagonist and Agonist Cycle
1
6
EN
Z.Candan
İltemir Duvan
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey
Müberra
Namlı Kalem
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey
muberranamli@hotmail.com
Yuksel
Onaran
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey
Esra
Aktepe Keskin
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey
Aylin
Ayrım
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey
Aslıhan
Pekel
Department of IVF-Embriology, School of Medicine, Turgut Ozal University, Ankara, Turkey
Hasan
Kafalı
Department of Obstetrics and Gynaecology, School of Medicine, Turgut Ozal University, Ankara, Turkey;Department of Obstetrics and Gynaecology, School of Medicine, Gazi University, Ankara, Turkey
Nilgün
Turhan
4Department of Obstetrics and Gynaecology, School of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey
10.22074/ijfs.2016.5144
<strong>Background</strong><br /> Coasting can reduce the ovarian hyperstimulation syndrome (OHSS) risk in ovulation induction cycles before intracytoplasmic sperm injection (ICSI). This study aimed to investigate the effect of gonadotropin-releasing hormone (GnRH) agonist and GnRH antagonist protocols to controlled ovarian hyperstimulation (COH) cycles with coasting on the parameters of ICSI cycles and the outcome. <br /><strong>Materials and Methods</strong><br /> In a retrospective cohort study, 117 ICSI cycles were per- formed and coasting was applied due to hyperresponse, between 2006 and 2011. The ICSI outcomes after coasting were then compared between the GnRH agonist group (n=91) and the GnRH antagonist group (n=26). <br /><strong>Results</strong><br /> The duration of induction and the total consumption of gonadotropins were found to be similar. Estradiol (E2) levels on human chorionic gonadotropin (hCG) day were found higher in the agonist group. Coasting days were similar when the two groups were compared. The number of mature oocytes and the fertilization rates were similar in both groups; however, the number of grade 1 (G1) embryos and the number of transferred embryos were higher in the agonist group. Implantation rates were significantly higher in the antagonist group compared to the agonist group. Pregnancy rates/embryo transfer rates were higher in the antagonist group; however, this difference was not statistically significant (32.8% for agonist group vs. 39.1% for antagonist group, P > 0.05). <br /><strong>Conclusion</strong><br /> The present study showed that applying GnRH-agonist and GnRH-antago- nist protocols to coasted cycles did not result in any differences in cycle parameters and clinical pregnancy rates.
Ovarian Hyperstimulation Syndrome,GnRH Agonist,GnRH Antagonist
https://www.ijfs.ir/article_45407.html
https://www.ijfs.ir/article_45407_f6a228ef9785a8a19a06d813ec605d95.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Low-Dose Urinary Human Chorionic Gonadotropin Is Effective for Oocyte Maturation in In Vitro Fertilization/ Intracytoplasmic Sperm Injection Cycles Independent of Body Mass Index
7
14
EN
Luis
R. Hoyos
. Department of Obstetrics and Gynecology, Wayne State University School of Medicine,
Detroit Medical Center, Detroit, MI, USA
Sana
Khan
. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology,
Wayne State University School of Medicine, Detroit Medical Center, Detroit, MI, USA
Jing
Dai
. C.S. Mott Center for Human Growth and Development, Wayne State University School of Medicine,
Detroit Medical Center, Detroit, MI, USA
Manvinder
Singh
. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology,
Wayne State University School of Medicine, Detroit Medical Center, Detroit, MI, USA
Michael
P. Diamond
4. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology,
Augusta University, Augusta, GA, USA
michael.diamond@augusta.edu
Elizabeth
E. Puscheck
. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology,
Wayne State University School of Medicine, Detroit Medical Center, Detroit, MI, USA
Awoniyi
O. Awonuga
. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology,
Wayne State University School of Medicine, Detroit Medical Center, Detroit, MI, USA
dx2350@wayne.edu
10.22074/ijfs.2016.5145
Background: Currently, there is no agreement on the optimal urinary derived human chorionic gonadotropin (u-hCG) dose requirement for initiating final oocyte maturation prior to oocyte collection in in vitro fertilization (IVF), but doses that range from 2500- 15000 IU have been used. We intended to determine whether low dose u-hCG was effective for oocyte maturation in IVF/intracytoplasmic sperm injection (ICSI) cycles independent of body mass index (BMI). Materials and Methods: We retrospectively evaluated a cohort of 295 women who underwent their first IVF/ICSI cycles between January 2003 and December 2010 at the Division of Reproductive Endocrinology and Infertility, Wayne State University, Detroit, MI, USA. Treatment cycles were divided into 3 groups based on BMI (kg/ m2):
Body Mass Index,Urinary Human Chorionic Gonadotropin,Fertilization,Pregnancy Rate,Live Birth Rate
https://www.ijfs.ir/article_45408.html
https://www.ijfs.ir/article_45408_bcf5263bea8155b3454bffa34e672af2.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Renal and Hepatic Functions after A Week of Controlled Ovarian Hyperstimulation during In Vitro Fertilization Cycles
15
19
EN
Ilaria
Romito
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
romitoilaria@gmail.com
Ferdinando Antonio
Gulino
0000-0001-7910-0386
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
docferdi@hotmail.it
Antonio Simone
Laganà
. Unit of Gynecology and Obstetrics, Department of Human Pathology in Adulthood and Childhood “G. Barresi”,
University of Messina, Messina, Italy
antlagana@unime.it
Salvatore Giovanni
Vitale
. Unit of Gynecology and Obstetrics, Department of Human Pathology in Adulthood and Childhood “G. Barresi”,
University of Messina, Messina, Italy
Attilio
Tuscano
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
attiliotuscano@gmail.com
Gianluca
Leanza
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
gianlucamed@gmail.com
Giulia
Musmeci
. Department of Experimental and Clinical Pharmacology, University of Catania, Catania, Italy
giuli.musmeci@outlook.it
Vito
Leanza
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
vito.leanza@gmail.com
Agnese Maria Chiara
Rapisarda
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
agni.rap@live.it
Marco Antonio
Palumbo
. Department of General Surgery and Medical Surgical Specialties, University of Catania, Catania, Italy
marcoant.palumbo@tiscali.it
10.22074/ijfs.2016.4689
Background: One the main aspects of in vitro fertilization (IVF) cycle is to avoid any possible systemic damage on women undergoing a controlled ovarian hyperstimulation (COH). The aim of this work is to evaluate renal and hepatic function blood tests in patients undergoing controlled ovarian hyperstimulation during IVF cycles. Materials and Methods: We performed a prospective cohort analysis. All patients re- ceived a long stimulation protocol with gonadotropin-releasing hormone (GnRH) analogues by daily administration, since the twenty-first day of the previous ovarian cycle followed by COH with recombinant follicle-stimulating hormone (FSH). The daily dose of exogenous gonadotropins for every single patient was modified according to her follicular growth. The oocytes were retrieved during the oocyte pick up and fertilized by standard procedures of intracytoplasmic sperm injection (ICSI). The blood samples to evaluate renal and hepatic functions were taken at the 7th day of ovarian stimulation. Results: We enrolled 426 women aged between 19 and 44 years, with a mean body mass index (BMI) of 24.68 Kg/m2. The mean value of blood urea nitrogen was 14 ± 3.16 mg/ dl, creatinine: 1 ± 0.45 mg/dl, uric acid: 4 ± 1.95 mg/dl, total proteins: 7 ± 3.93 mg/dl, aspartate aminotransferase: 18 ± 6.29 mU/ml, alanine aminotransferase: 19 ± 10.41 mU/ ml, alkaline phosphatase: 81 ± 45.25 mU/ml, total bilirubin 1 ± 0.35 mg/dL. All of the results were considered as a normal range following the Medical Council of Canada. Conclusion: Our data suggest that, unlike ovarian hyperstimulation syndrome (OHSS), COH patients did not show any alteration to renal and hepatic functions.
infertility,ovarian hyperstimulation,Intracytoplasmic Sperm Injection,Fertilization
https://www.ijfs.ir/article_45409.html
https://www.ijfs.ir/article_45409_84657df9b427578b37416b2c47fb92c9.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Identification of Reproductive Education Needs of Infertile Clients Undergoing Assisted Reproduction Treatment Using Assessments of Their Knowledge and Attitude
20
27
EN
Zahra
Ezabadi
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
Fahimeh
Mollaahmadi
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran
mollaahmadi@royaninstitute.org
Maryam
Mohammadi
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
maryambluebutterfly@gmail.com
Reza
Omani Samani
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
info@royaninstitet.org
Samira
Vesali
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
samiravesali@yahoo.com
10.22074/ijfs.2016.4728
<strong>Background</strong><br /> In order to empower infertile individuals and provide high quality patient-centered infertility care, it is necessary to recognize and meet infertile individuals’ educational needs. This study aims to examine infertility patients’ knowledge and subsequently their education needs given their attitudinal approach to infertility education in terms of patients who undergo assisted reproduction treatment. <br /><strong>Materials and Methods</strong><br /> This descriptive study enrolled 150 subjects by conveni- ence sampling of all patients who received their first assisted reproductive treatment between July and September 2015 at a referral fertility clinic, Royan Institute, Tehran, Iran. We used a questionnaire that measured fertility and infertility information (8 questions) as well as attitude toward education on the causes and treatment of infertility (5 questions). Chi-square, independent sample t test, and one way ANOVA analyses were conducted to examine differences by sex. P < 0.05 was considered statistically significant. <br /><strong>Results</strong><br /> Total mean knowledge was 3.08 ± 0.99. Clients’ responses indicated that the highest mean knowledge scores related to knowledge of factors that affected pregnancy (3.97 ± 1.11) and infertility treatment (3.97 ± 1.16). The lowest mean knowledge scores related to knowledge of the natural reproductive cycle (2.96 ± 1.12) and anatomy of the genital organs (2.94 ± 1.16). Most females (92.1%) and males (83.3%) were of the opinion that infertility education programs should include causes of infertility and types of treatment associated with diagnostic and laboratory procedures. No statistically significant difference existed between male and female participants (P=0.245). <br /><strong>Conclusion</strong><br /> Most participants in this study expressed awareness of factors that affect pregnancy and infertility treatment. It is imperative to educate and empower infertile individuals who seek reproduction treatment in terms of infertility causes and types of treatment, as well as diagnostic and laboratory procedures to enable them to make informed decisions about their assisted reproductive procedures.
education,Training,Knowledge,Attitude,infertility
https://www.ijfs.ir/article_45410.html
https://www.ijfs.ir/article_45410_6d51018be141c1d1efed9a83efb188b9.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
The Frequency of Staphylococcus aureus Isolated from Endocervix of Infertile Women in Northwest Iran
28
32
EN
Mohammad Taghi
Akhi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
m_t_akhi@yahoo.com
Aylin
Esmailkhani
Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
a.esmailkhani@gmail.com
Javid
Sadeghi
Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
sadeghij@tbzmed.ac.ir
Behrooz
Niknafs
Department of Anatomical Sciences, Medical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
niknafsbeh@yahoo.com
Laya
Farzadi
4Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Aydin
Akhi
5Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
aydin_akhi@yahoo.com
Elmira Najafi
Nasab
Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
10.22074/ijfs.2016.4969
<strong>Background</strong><br /> Infertility is one of the major social issues. Due to the asymptomatic cervical infection associated with Staphylococcus aureus (S. aureus), the majority of patients remain undiagnosed. The present study intended to assess the frequency of S. aureus isolated from infertile women’s endocervix in northwest Iran. <br /><strong>Materials and Methods</strong><br /> In a descriptive cross sectional study, specimens were randomly collected during vagina examination using a sterile speculum and swabbing. After performance of antibiotic susceptibility testing, polymerase chain reaction (PCR) was used to identify methicillin-resistance S. aureus (MRSA) and toxic shock syndrome toxin-1 (TSST-1). <br /><strong>Results</strong><br /> About 26 (26%) and 9 (9%) women’s urogenital tracts were colonized by S. aureus and Candida spp., respectively, of which three (11.5%) patients were infected with fungi and S. aureus, simultaneously. Antibiotic susceptibility results showed high activity of vancomycin and co-trimoxazole on isolates. Regarding PCR results, mecA sequences were detected in 7 (26.9%) strains, whilst the tst gene encoding TSST-1 was not detected in any of clinical strains. <br /><strong>Conclusion</strong><br /> The prevalence of S. aureus was very high in infertile women. Therefore, it demands all patients undergoing infertility treatment to be investigated thoroughly for this type of infection.
infertility,Endocervix
https://www.ijfs.ir/article_45411.html
https://www.ijfs.ir/article_45411_378848e50e124ad308869b9c67b20983.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Follicle Stimulating Hormone and Anti-Müllerian Hormone among Fertile and Infertile Women in Ile-Ife, Nigeria: Is there A Difference?
33
39
EN
Temitope
Okunola
Department of Obstetrics and Gynecology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria
bezaleelokunola@yahoo.com
Kayode
Olusegun Ajenifuja
Obafemi Awolowo University, Ile-Ife, Nigeria
Olabisi
Morebise Loto
Obafemi Awolowo University, Ile-Ife, Nigeria
Afolabi
Salawu
Ladoke Akintola University of Technology Teaching Hospital, Ogbomoso, Nigeria
afolabi@yahoo.com
Stephen Oluseyi
Omitinde
Department of Obstetrics and Gynecology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria
10.22074/ijfs.2016.4645
<strong>Background</strong><br /> Reduced ovarian reserve predicts poor ovarian response and poor suc- cess rates in infertile women who undergo assisted reproductive technology (ART). Ovarian reserve also decreases with age but the rate of decline varies from one woman to another. This study aims to detect differences in ovarian reserve as measured by basal serum follicle stimulating hormone (FSH) and anti-Müllerian hormone (AMH) between a matched cohort of fertile and infertile regularly menstruating women, 18-45 years of age. <br /><strong>Materials and Methods</strong><br /> This case-control study involved 64 fertile and 64 subfertile women matched by age at recruitment. Peripheral blood samples were taken from the women recruited from the Gynecological and Outpatient Clinics of Obafemi Awolowo University Teaching Hospital, Ile-Ife, Nigeria. Serum FSH and AMH were quantified using ELISA at the Metabolic Research Laboratory of LAUTECH Teaching Hospital, Ogbomoso, Nigeria. <br /><strong>Results</strong><br /> A significant difference existed in the mean FSH of fertile (6.97 ± 3.34) and infertile (13.34 ± 5.24, P=0.013) women. We observed a significant difference in AMH between fertile (2.71 ± 1.91) and infertile (1.60 ± 2.51, P=0.029) women. There was a negative correlation between FSH and AMH in both fertile (r=-0.311, P=0.01) and infertile (r=-0.374, P=0.002) women. <br /><strong>Conclusion</strong><br /> The difference in ovarian reserve observed in this study suggests that reduced ovarian reserve in regularly menstruating women may be associated with early ovarian ageing or subfertility.
infertility,OVARIAN RESERVE,Follicle Stimulating Hormone,Anti,Müllerian Hormone
https://www.ijfs.ir/article_45412.html
https://www.ijfs.ir/article_45412_3f5fd6b1cc5bd1d734e3c9c034bf95c2.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Age as A Predictor of Embryo Quality Regardless of The Quantitative Ovarian Response
40
46
EN
Juliano Brum.
Scheffer
Brazilian Institute of Assisted Reproduction, Belo Horizonte, Brazil
drjulianoscheffer@gmail.com
Bruno Brum.
Scheffer
Brazilian Institute of Assisted Reproduction, Belo Horizonte, Brazil
brunobrumscheffer@hotmail.com
Rafaela Friche
de Carvalho
Brazilian Institute of Assisted Reproduction, Belo Horizonte, Brazil
Joyce
Rodrigues
Brazilian Institute of Assisted Reproduction, Belo Horizonte, Brazil
joyceufmg@yahoo.com.br
Michael
Grynberg
Department of Reproductive Medicine, Hospital Jean Verdier (AP-HP), University Paris XIII,
and INSERM, Paris, France
michael.grynberg@gmail.com
Daniel H.
Mendez Lozano
School of Medicine, Tecnológico de Monterrey and Center for Reproductive Medicine
CREASIS San Pedro Monterrey, México
10.22074/ijfs.2016.4579
<strong>Background</strong><br /> One determining factor of a successful in vitro fertilization (IVF) cycle is embryo quality. The aim of the present study was to evaluate associations of embryo quality and reserve markers like age, FSH and AMH. <br /><strong>Materials and Methods</strong><br /> In this prospective study, 120 infertile women, aged 21-44 years, undergoing routine exploration during an unstimulated cycle preceding assisted reproductive technology (ART) at our center were studied prospectively, from February 2011 to December 2014. Descriptive parameters and patient characteristics were reported as mean (SD) or median (range) depending on the distribution. Student’s t test was performed for continuous variables, Wilcoxon and Pearson’s Test were used for not distributed variables and Fisher’s Test was performed for categorical variables. P < 0.05 was considered statistically significant. <br /><strong>Results</strong><br /> Overall, at the time of investigation, patients had a mean age of 33.03 ± 4.15 years old. On cycle day three, serum anti-Mullerian hormone (AMH) level was 3.50 ± 1.54 ng/mL, serum follicle-stimulating hormone (FSH) level was 6.29 ± 1.53 mUI/ mL, at baseline, women had 16.57 ± 7.0 antral follicles. The mean of collected oocytes was 11.80 ± 5.25, embryo I+II was 2.46 ± 2.11. A greater number of embryos I+II was observed in young patients. By evaluating 120 patients, a significant relationship was observed between age and FSH (r=0.24, P=0.01), age with AMH (r=-0.22, P=0.02), age with collected oocytes (r=-0.23, P=0.03) and age with embryo I+II (r=-0.22, P=0.03). A significant relationship was also observed between antral follicle count (AFC) and AMH (r=0.29, P=0.01), AFC and the number of transferred embryo (r=-0.18, P=0.03), AFC and total dose of the drugs (r=-0.23, P=0.03). Significant relationship of FSH with total dose of drugs (r=0.19, P=0.02) was also observed. In addition, we determined significant relationships between AMH and the number of collected oocytes (r=0.38, P=0.01), AMH and the number of metaphase II oocytes (r= 0.35, P=0.01), AMH and the number of embryo (r=0.19, P=0.04) as well as AMH and total dose of the drugs (r=-0.25, P=0.01). <br /><strong>Conclusion</strong><br /> Commonly used clinical markers of ovarian reserve are reflection of the ovarian reserve, while the outcome measurements of ART and age are the best predictors of embryo quality.
age,Anti,Mullerian Hormone,Follicle,Stimulating Hormone
https://www.ijfs.ir/article_45413.html
https://www.ijfs.ir/article_45413_db79a40afe7e6dc2e977d07d63653a6a.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Changes of The Uterine Tissue in Rats with Polycystic Ovary Syndrome Induced by Estradiol Valerate
47
55
EN
Ghadire
Mirabolghasemi
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
mirabolghasemi@khu.ac.ir
Zahra
Kamyab
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
kamyab-zahra@yahoo.com
10.22074/ijfs.2016.4794
<strong>Background</strong><br /> Polycystic ovary syndrome (PCOS) is one of the most common hormonal disorders that can lead to irregular menstrual cycles and hyperandrogenism. Reduced levels of progesterone and increased estrogen in these women can perpetually stimulate the endometrial tissue of the uterus. In this study, we assess the effect of PCOS induction by estradiol valerate (EV) in a rat model. <br /><strong>Materials and Methods</strong><br /> In this experimental study, adult female Wistar rats that weighed approximately 200 g were divided into control, sham, and experimental groups (n=6 per group). The experimental group received subcutaneous injections of 2 mg EV for induction of PCOS. We confirmed the presence of PCOS in the experimental group rats. Rats from all groups were subsequently killed, after which their uteri were removed and fixed for histological and cytological analyses. The uterine tissue sections were stained with hematoxylin and eosin (H&E) and iron hematoxylin (iron-H). We examined epithelium height, thickness of the uterus wall, and frequency of the mitotic cells. The data were assessed at α=0.05. <br /><strong>Results</strong><br /> Uterine tissue findings from the experimental group showed significant increases in the height of the uterus luminal epithelium, the thickness of the uterus wall, and the frequency of eosinophils in the endometrial stroma. We observed an increased frequency of mitotic cells in the experimental group in both luminal and glandular epithelia of the uterus. An increased rate of the glandular epithelium region was noticeable and significant. <br /><strong>Conclusion</strong><br /> Induction of PCOS by EV could change the proliferation rate in the endo- metrial tissue of the uterus.
Uterus,Estradiol valerate,Polycystic Ovary Syndrome,Mitosis,Rat
https://www.ijfs.ir/article_45414.html
https://www.ijfs.ir/article_45414_c967343f169a65de4a765a7e4d671e49.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
International Journal of Fertility and Sterility
2008-076X
2008-0778
11
1
2017
04
01
Bull Fertility and Its Relation with Density Gradient Selected Sperm
55
62
EN
Lynda
Allouche
Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, Ferhat Abbas Setif 1 University,
Setif, Algeria
lyallouche@gmail.com
Toufik
Madani
Department of Agronomy, Faculty of Nature and Life Sciences, Ferhat Abbas Setif 1 University, Setif, Algeria
madani2000dz@yahoo.fr
Mohamed
Mechmeche
National Center for Artificial Insemination and Genetic Improvement, Algiers, Algeria
vetoconseils@yahoo.fr
Laetitia
Clement
4National Laboratory for Health Control in Breeding stock (LNCR), Allice, Maison Alfort, France
laetitia.clement@lncr.org
Allaoua
Bouchemal
National Center for Artificial Insemination and Genetic Improvement, Algiers, Algeria
bouchemalla2@yahoo.fr
10.22074/ijfs.2016.4721
<strong>Background</strong><br /> Sperm selection method is usually used to collect these cells for in vitro-assisted reproduction. Few studies reported the relationship of in vivo fertility and semen parameters after sperm selection; hence, the present study attempted to assess different semen parameters after post-thaw or sperm selection, using density gradient separation BoviPure®, to predict in vivo fertility. <br /><strong>Materials and Methods</strong><br /> In this experimental study, frozen semen quality of four Montbeliarde bulls were assessed after post-thaw (PT) or after sperm selection (SSp), using density gradient separation BoviPure®, to predict the fertility rate in vivo. In addition to PT or SSp, semen was examined for concentration, motility, morphology abnormalities, viability, acrosome and plasma membrane integrities. Fertility was measured as non-return rates within 56 days after the first insemination (NRR) or as corrected NRR, expressed as CNRR, to the factors influencing fertility using linear mixed model. Non-parametric Kruskal-Wallis test was performed to compare semen parameter variables. Fertility rates were compared using Chi-square test. Pearson correlation analysis was used to test the relationship between CNRR and semen parameters. Data was analysed using SPSS package program, version 21.0. <br /><strong>Results</strong><br /> Most of the examined bulls exhibited a high fertility rate (3/4 bulls, 62.1- 81.8% for NRR or 67.2-98.5% for CNRR). Fertility rate, expressed as CNRR, was significantly related to semen parameters after SSp, but not after PT. Thus, CNRR was increased with decrease of total motility, progressive spermatozoa and abaxial implantation frequencies after SSp (r=-0.999, P=0.001; r=-0.990, P=0.010; r=-0.988, P= 0.012, respectively); while, CNRR was decreased with decrease of SSp immotile spermatozoa (r=+0.995, P=0.005), underlying that maximal limit of determined immotile spermatozoa is 47%. <br /><strong>Conclusion</strong><br /> High frequencies of total and progressive motility spermatozoa, and abaxial implantation in gradient selected sperm appear to be not favorable for fertility in vivo.
frozen semen,Fertility,Bull
https://www.ijfs.ir/article_45415.html
https://www.ijfs.ir/article_45415_ace8e1e51613bb53560ab491521db986.pdf