ORIGINAL_ARTICLE
Does Islamic Fasting Affect Gonadotropin around Female Ovulation?
Background The pattern of Islamic fasting differs from other forms of fasting, therefore its effect on health also differs. This research studies the effect of Islamic fasting on gonadotropin hormones around the time of the ovulatory cycle and ovulation. Materials and methods This self-controlled study was performed on 24 adult females. Blood sampling was performed during Ramadan and two months later to determine the levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen and progesterone. Ultrasonography was done in order to detect ovulation and the results were compared. Results The mean values of FSH, LH and estrogen during the 14th day of menstruation were comparable with non-fasting values. There was no significant difference in ovulation. Conclusion Islamic fasting causes neither significant variation in hormone secretion around ovulation nor does it influence the occurrence of ovulation.
https://www.ijfs.ir/article_45803_1ccbd2e4a955ea186e581fb7bf29ba7f.pdf
2010-10-01
94
97
10.22074/ijfs.2010.45803
Fasting
Islamic
Gonadotropin
Ovulation
Sima
Shahabi
1
Physiology and Pharmacology Department, Babol University of Medical Science, Babol, Iran
AUTHOR
Seddigheh
Esmaeilzadeh
2
Fatemeh-Zahra Infertility and Reproductive Health Research Center, Babol University of Medical Science, Babol, Iran
LEAD_AUTHOR
Masoumeh
Golsorkhtabar Amiri
3
Fatemeh-Zahra Infertility and Reproductive Health Research Center, Babol University of Medical Science, Babol, Iran
AUTHOR
Mahboubeh
Faramarzi
4
Obstetrics and Gynecology Department, Babol University of Medical Science, Babol, Iran
AUTHOR
Ali Reza
Firouzjahee
5
Hematology Department, Babol University of Medical Science, Babol, Iran
AUTHOR
Tayebeh
Esmaeili
6
Medical Statistics Department, Babol University of Medical Science, Babol, Iran
AUTHOR
ORIGINAL_ARTICLE
Fertilization and Embryo Development of Fresh and Cryopreserved Sibling Oocytes
Background The pattern of Islamic fasting differs from other forms of fasting, therefore its effect on health also differs. This research studies the effect of Islamic fasting on gonadotropin hormones around the time of the ovulatory cycle and ovulation. Materials and methods This self-controlled study was performed on 24 adult females. Blood sampling was performed during Ramadan and two months later to determine the levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen and progesterone. Ultrasonography was done in order to detect ovulation and the results were compared. Results The mean values of FSH, LH and estrogen during the 14th day of menstruation were comparable with non-fasting values. There was no significant difference in ovulation. Conclusion Islamic fasting causes neither significant variation in hormone secretion around ovulation nor does it influence the occurrence of ovulation.
https://www.ijfs.ir/article_45804_632a3e5d613818f5a2d18d80c7a4c93f.pdf
2010-10-01
98
103
10.22074/ijfs.2010.45804
Cryopreservation
Fertilization
Embryo Quality
ICSI
oocytes
Navid
Esfandiar
1
Toronto Centre for Advanced Reproductive Technology, TCART , Toronto, Canada
LEAD_AUTHOR
Murid H.
Javed
2
Toronto Centre for Advanced Reproductive Technology, TCART , Toronto, Canada
AUTHOR
Zohreh
Nazemian
3
Toronto Centre for Advanced Reproductive Technology, TCART , Toronto, Canada
AUTHOR
Robert F.
Casper
4
Toronto Centre for Advanced Reproductive Technology, TCART , Toronto, Canada
AUTHOR
ORIGINAL_ARTICLE
Can Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis?
Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. On the other hand, DNA damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with DNA fragmentation and chromatin abnormalities in human ejaculates is well documented, in particular in men with poor semen quality. Therefore, the aim of this study is to evaluate HSPA2 expression and its relation with DNA fragmentation, protamine deficiency involved in DNA packaging and semen parameters in varicocele patients in comparison to fertile men before and after varicocelectomy. Materials and methods This study included 52 fertile individuals as the control group and 70 infertile individuals with varicocele as the experimental group. Sperm DNA fragmentation, protamine deficiency and relative HSPA2 expression were evaluated by the sperm chromatin dispersion test, chromomycin A3 staining and RT-PCR, respectively. Results The mean values of abnormal morphology, protamine deficiency and DNA fragmentation were significantly lower in varicocele individuals following varicocelectomy when compared to fertile individuals. The correlation between these parameters were studied and discussed in the text. Conclusion There is a decrease in relative HSPA2 expression which is possibly due to chronic induced hyperthermia in varicocele individuals. Removal of this stress increases HSPA2 expression and results in the proper folding of proteins involved in spermatogenesis; therefore resulting in improved DNA packaging, as well as better sperm morphology and motility which may indirectly reduce sperm DNA fragmentation.
https://www.ijfs.ir/article_45805_3830e49e309f6d3c727fa5c51ba53062.pdf
2010-10-01
104
113
10.22074/ijfs.2010.45805
Varicocele
HSPA2
Protamines
DNA fragmentation
surgery
Mohammad Hossein
Nasr Esfahani
mh_nasr-esfahani@royaninstitute.org
1
Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran nimal Biotechnology, ACECR, Isfahan, Iran
LEAD_AUTHOR
Homayoun
Abbasi
2
Isfahan Fertility and Infertility Center, Isfahan, Iran
AUTHOR
Zahra
Mirhosseini
3
Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran nimal Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Nazem
Ghasemi
4
Department of Anatomy, Isfahan Medical University, Isfahan, Iran
AUTHOR
Shahnaz
Razavi
5
Department of Anatomy, Isfahan Medical University, Isfahan, Ira
AUTHOR
Marziyeh
Tavalaee
6
Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran nimal Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Somayeh
Tanhaei
7
Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Mohammad Reza
Deemeh
8
Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran nimal Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Kamran
Ghaedi
9
Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran iotechnology, ACECR, Isfahan, Iran
AUTHOR
Farzaneh
Zamansoltani
10
Department of Anatomy, Ghazvin Medical University, Ghazvin, Iran
AUTHOR
Farzad
Rajaei
11
Department of Anatomy, Ghazvin Medical University, Ghazvin, Iran
AUTHOR
ORIGINAL_ARTICLE
Profound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique
Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. Materials and methods This was a nested case-control study. Ten sperm samples were obtained from infertile patients [n=5 (two aliquots each from five samples; one before the swim-up process and one after)] and donors [n=5 (two aliquots from five samples, one before the swim-up process and one after)]. Oligonucleotide microarrays were employed to study the genome-wide expression of pooled samples from infertile patients vs. donors. A total of four microarrays were perfomed: two with sperm sample aliquots before swim-up and two with sperm samples aliquots after swim-up, from both the case and control groups. The results were evaluated to detect which genes expressed differentially [fold change (FC)>5 and p <0.05] and which genes were exclusive to each of the groups, both before and after swim-up. Results Profound differences were detected between the fresh sperm samples of donors vs. infertile patients with respect to both differentially and exclusively expressed genes. Nevertheless these differences seemed to decrease after the swim-up selection process. Conclusion There are important differences between the expression profiles of sperm samples of fertile donors vs. infertile patients who require assisted reproduction techniques (ART). These differences are potential forecasters of fertility success, although their reliability needs to be explored further.
https://www.ijfs.ir/article_45807_56225a51f928a7d4ae9851e4eece379f.pdf
2010-10-01
114
121
10.22074/ijfs.2010.45807
Gene expression
infertility
Microarray Analysis
Spermatozoa
Sandra
García-Herrero
1
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
LEAD_AUTHOR
Nicolás
Garrido
2
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
AUTHOR
José Antonio
Martínez-Conejero
3
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
AUTHOR
José
Remohí
4
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
AUTHOR
Antonio
Pellicer
5
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
AUTHOR
Marcos
Meseguer
6
Valencian Infertility Institute (IVI), Valencia University, Valencia, Spain
AUTHOR
ORIGINAL_ARTICLE
Evaluation of the Prevalence of Polycystic Ovarian Syndrome among Adolescent (15-18 Years Old) Girls in Tehran during 2005-2006
Background Polycystic ovarian syndrome (PCOS) was first introduced by Leventhal and Stein in 1935. It has a wide range of manifestations such as hyperandrogenism, irregular menstruation and central body obesity. The lack of definite diagnostic criteria has made the diagnosis a difficult task. Moreover, the disturbing aspects of the syndrome range from hirsutism in an adolescent girl to infertility in a young woman. Based on the Rotterdam 2003 Criteria, current diagnostic criteria must include two of the following three symptoms: 1. Either oligo-ovulation or anovulation, 2. Clinical or laboratory manifestations of hyperandrogenism, 3. Polycystic ovaries on sonographic examination (more than 12 follicles of less than 10 mm in diameter in each ovary). In this study, we have focused on the enrollment of a large sample size to assess the prevalence and clinical features of PCOS such that a wide range of basic information can be utilized for further investigations. Materials and methods This was a cross-sectional study with multi-stage random sampling. Subjects were selected from 15-18 years old girls from a number of high schools in Tehran. After interview and clinical examination, those individuals with either menstrual irregularities, hirsutism or obesity were referred for further laboratory evaluations and abdominal sonography in order to diagnose PCOS and estimate its prevalence. We also aimed to assess the predominant features of this disorder among adolescents. Results The overall prevalence of the syndrome was 3.42%. Out of the 1430 girls, 49 were diagnosed with PCOS. Conclusion Despite its high prevalence, this syndrome has not been widely studied. Most of the conducted studies have enrolled small samples within a short time period. Thus, more accurate policy making in this regard can be achieved through definition of the prevalence of PCOS and accurate estimation of its burden.
https://www.ijfs.ir/article_45808_3cfa692a659f21ff78d6bae8f5827778.pdf
2010-10-01
122
127
10.22074/ijfs.2010.45808
Saghar
Salehpour
saghar_salehpour@yahoo.com
1
Infertility and Reproductive Health Research Center, Taleghani Training Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Hamidreza
Esmaeilnia Shirvani
2
Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Abbas
Entezari
3
Infertility and Reproductive Health Research Center, Taleghani Training Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
The Prevalence of Chlamydia trachomatis Infection in Women Attending Health Clinics in Shiraz, Islamic Republic of Iran
Background Chlamydia trachomatis (CT) is a common cause of sexually transmitted infections (STI) and a prevalent microorganism found in the vaginal discharge of sexually active women. The infection usually has no symptoms; although it may cause chronic complications such as pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain, tubal infertility and cervical cancer. This study was done, for the first time, in Shiraz (southern Iran) to determine the prevalence of genital C.T in women Materials and methods A total of 402 women who referred for routine pap smears to randomly selected health centers in Shiraz were tested with the IMAGEN Chlamydia test, a direct immunofluorescence test for the detection of Chlamydia in human urogenital specimens. The results of this test were compared to the socio-demographic condition of each participant as well as their sexually transmitted disease (STD) symptoms, both present and past. Results The prevalence rate of Chlamydia infection was 8% (32/402). No correlation was found between this infection and age, marital age, number of children, education and occupational status of the participants. On the other hand, the correlation between the presence of infection and a positive history of STD symptoms, both present and past was significant. Conclusion Chlamydia infection is quite frequent in our society, where knowledge about STD infections is scant. Considering the sequelae of this infection and the fact that this is a quiet disease, it seems rational to screen sexually active women and inform them more about this issue.
https://www.ijfs.ir/article_45809_f360697b473f4aab7ef1c39db51902e9.pdf
2010-10-01
128
133
10.22074/ijfs.2010.45809
sexually transmitted infection
Chlamydia trachomatis
Direct Immunofluorescence
Endocervical Specimen
Negin
Hadi
hadin@sums.ac.ir
1
Department of Community Medicine, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
Fariba
Barazandeh
2
Shiraz Emergency Medical Services, Shiraz, Iran
AUTHOR
Fariborz
Azad
3
Immunology Lab, Department of Pathology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
ORIGINAL_ARTICLE
C677T and A1298C Mutations in the Methylenetetrahydrofolate Reductase Gene in Patients with Recurrent Abortion from the Iranian Azeri Turkish
Background To assess whether the C677T and A1298C mutations in the methylenetetrahydrofolate reductase (MTHER) gene are associated with recurrent abortion (RA), we determined the frequencies of the T677 and C1298 mutations in patients and controls. Materials and methods Mutations were determined by a RFLP-PCR method in 53 patients and 61 matched controls. Results The frequencies of T alleles were 0.26 in patients and 0.29 in controls. The frequencies of C/C, T/C and T/T genotypes were 34 (55.7%), 22 (36.1%) and 5 (8.2%) in patients, and 27 (50.9%), 21 (39.6%) and 5 (9.43%) in controls. The C allele frequencies were 0.38 in patients and controls. C/C, A/C and A/A genotype distributions were 9 (14.8%), 28 (45.9%) and 24 (39.3%) in patients, and 8 (15.1%), 24 (45.3%) and 21 (39.6%) in controls. Conclusion There were no significant differences between patients and controls concerning the T677 and C1298 mutations.
https://www.ijfs.ir/article_45810_b56cfe096d8ae47ad83bfc18e4c2627d.pdf
2010-10-01
134
139
10.22074/ijfs.2010.45810
MTHFR
Pregnancy
Recurrent abortion
Morteza
Bagheri
mortezabagheri@umsu.ac.ir
1
Genetics Department, Urmia University of Medical Sciences, Urmia, Iran
LEAD_AUTHOR
Isa
Abdi Rad
2
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
AUTHOR
Mir Davood
Omrani
3
Genetics Department, Urmia University of Medical Sciences, Urmia, Iran
AUTHOR
Fariba
Nanbakhsh
4
Obstetrics and Gynecology Department, Urmia University of Medical Sciences, Urmia, Iran
AUTHOR
ORIGINAL_ARTICLE
Ectopic Pregnancy in Cesarean Section Scar
Pregnancy implantation within the scar of a previous cesarean delivery is one of the rarest locations for an ectopic pregnancy. Early diagnosis of this condition with the use of ultrasound imaging allows for preserving the uterus and subsequent fertility. However, a delay in either diagnosis or treatment can lead to uterine rupture, hysterectomy and significant maternal morbidity. With increasing incidence of cesarean sections (CS) worldwide and more liberal use of transvaginal sonography (TVS) in early pregnancy, more cases of cesarean scar pregnancy (CSP) are diagnosed. Thus every woman with a previous CS presenting to the early pregnancy unit should have a routine check for the cesarean scar appearance. We present the case of a 34 year-old multigravid woman who was found to have an ectopic pregnancy in a cesarean scar at six weeks gestation, with significantly elevated beta-human chorionic gonadotrophin (Beta-hCG) level.
https://www.ijfs.ir/article_45811_83093cbd1bb76d49e4fab1649a160c47.pdf
2010-10-01
140
142
10.22074/ijfs.2010.45811
Cesarean Section
Ectopic pregnancy
Pregnancy
Firoozeh
Ahmadi
1
Reproductive Imaging Department, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
LEAD_AUTHOR
Fatemeh
Zafarani
2
Reproductive Imaging Department, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
AUTHOR
Hadieh
Haghighi
3
Reproductive Imaging Department, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
AUTHOR
Maryam
Niknejadi
mniknejadi@royaninstitute.org
4
Reproductive Imaging Department, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
AUTHOR