ORIGINAL_ARTICLE
Coding and Non-Coding RNAs, as Male Fertility and Infertility Biomarkers
Semen analysis is usually the first step in the assessment of male fertility. Although analyzes provide valuable information about male fertility, success of cytoplasmic sperm injection using this method is not predictable. In the recent years, studies have shown that sperm quality assessment helps clinicians predict male fertility status based on the expression of biomarkers. To write this article, a comprehensive study was conducted on several RNA transcripts by searching related words on medical information databases by 2018. According to the literature, spermatogenesis based disorders in male infertility have a significant relationship with the expression level of some RNA molecules (like DAZ and PRM1/PRM2 ratio) in semen and testicular tissue. Thus, they might be used as predictor biomarkersto evaluate success rate of testicular sperm extraction (TESE) procedure, but confirmation of this hypothesis requires more extensive research. By comparing the number of RNAs attributed to each fertility disorder in men, it is possible to trace the causes of disease or return fertility to some infertile patients by regulating the mentioned molecules. Further researches can provide a better understanding of the use of RNA expression profiles in the diagnosis and treatment of male infertility.
https://www.ijfs.ir/article_241304_4d19da4f3f3ba5d04280edefc2e4ee41.pdf
2021-07-01
158
166
10.22074/ijfs.2021.134602
Male infertility
Semen
Spermatogenesis
Fereshteh
Aliakbari
faezeh.azizi@sbmu.ac.ir
1
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Nahal
Eshghifar
n.eshghifar@gmail.com
2
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Reza
Mirfakhraie
reza_mirfakhraie@yahoo.com
3
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Parisa
Pourghorban
4
Department of Biology, Faculty of Biological Sciences, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
AUTHOR
Faezeh
Azizi
faezehazizi1@gmail.com
5
Non-Communicable Disease Control Department, Public Health Department, Minis try of Health and Medical Education, Tehran, Iran
LEAD_AUTHOR
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68
ORIGINAL_ARTICLE
Effects of Three-Dimensional Sodium Alginate Scaffold on Maturation and Developmental Gene Expressions in Fresh and Vitrified Preantral Follicles of Mice
Background: Prior to chemotherapy interventions, in vitro maturation (IVM) of folliclesthrough vitrification can be used to help young people conserve their fertility. Materials and Methods: This experimental study was conducted on immature female BALB/c mice (12-14 days). Follicles were gathered mechanically and placed in α-Minimal Essential Medium (α-MEM) containing 5% fetal bovine serum (FBS). Some pre-antral follicles were frozen. The fresh and vitrified follicles were cultured in different concentrations of sodium alginate (0.25%, 0.5%, and 1%) and two dimensional (2D) medium for 12 days. The samples were evaluated for viability percentage, the number of MII-phase oocytes and reactive oxygen specious (ROS) level. Additionally, Gdf9, Bmp15, Bmp7, Bmp4, Gpx, mnSOD and Gcs gene expressions were assessed in the samples. Results: The highest and lowest percentages of follicle viability and maturation in the fresh and vitrified groups were respectively 0.5% concentration and 2D culture. There was no significant difference among the concentrations of 0.25% and 1%. Viability and maturation of follicles showed a significant increase in the fresh groups in comparison with the vitrified groups. ROS levels in the both fresh and vitrified groups with different concentrations of alginate showed a significant decrease compared to the control group. ROS levels in follicles showed a significant decrease in the fresh groups in comparison with the vitrified groups (P≤0.0001). The highest gene expression levels were observed in the 0.5% alginate (P≤0.0001). Moreover, the viability percentage, follicle maturation, and gene expression levels were higher in the fresh groupsthan the vitrified groups (P≤0.0001). Conclusion: Alginate hydrogel at a proper concentration of 5%, not only helps follicle get mature, but also promotes the expression of developmental genes and reducesthe level of intracellular ROS. Follicular vitrification decreases quality of the follicles, which are partially compensated using a three dimensional (3D) cell culture medium.
https://www.ijfs.ir/article_45672_96d95fa132b6d89a3b5c0987f7ed354f.pdf
2021-07-01
167
177
10.22074/ijfs.2020.134609
Oocyte Maturation
Sodium alginate
Vitrification
Cyrus
Jalili
cjalili@yahoo.com
1
Medical Biology Research Center, Health Technology Ins titute, Kermanshah University of Medical Sciences, Kermanshah, Iran
AUTHOR
Fuzieh
Khani Hemmatabadi
fuziekhani@yahoo.com
2
Medical Biology Research Center, Health Technology Ins titute, Kermanshah University of Medical Sciences, Kermanshah, Iran
LEAD_AUTHOR
Mehrdad
Bakhtiyari
mehr_bakhtiyari@yahoo.com
3
Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
Amir
Abdolmaleki
enjoyanatomy@gmail.com
4
Medical Biology Research Center, Health Technology Ins titute, Kermanshah University of Medical Sciences, Kermanshah, Iran
AUTHOR
Fatemeh
Moradi
f7moradi@gmail.com
5
Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
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25. Peng J, Li Q, Wigglesworth K, Rangarajan A, Kattamuri C, Peterson RT, et al. Growth differentiation factor 9: bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions. Proc Natl Acad Sci USA. 2013; 110(8): E776-E785.
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26. Parrish EM, Siletz A, Xu M, Woodruff TK, Shea LD. Gene expression in mouse ovarian follicle development in vivo versus an ex vivo alginate culture sys tem. Reproduction. 2011; 142(2): 309-318.
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27. Sondermeijer HP, Witkowski P, Woodland D, Seki T, Aangenendt FJ, van der Laarse A, et al. Optimization of alginate purification using polyvinylidene difluoride membrane filtration: effects on immunogenecity and biocompatibility of three-dimensional alginate scaffolds. J Biomater Appl. 2016; 31(4): 510-520.
27
28. Wes t-Farrell ER, Xu M, Gomberg MA, Chow YH, Woodruff TK, Shea LD. The mouse follicle microenvironment regulates antrum formation and s teroid production: alterations in gene expression profiles. Biol Reprod. 2009; 80(3): 432-439.
28
29. Silva JR, van den Hurk R, Figueiredo JR. Ovarian Follicle development in vitro and oocyte competence: advances and challenges for farm animals. Domes t Anim Endocrinol. 2016; 55: 123-35.
29
30. Xu J, Lawson MS, Yeoman RR, Molskness TA, Ting AY, S touffer RL, et al. Fibrin promotes development and function of macaque primary follicles during encapsulated three-dimensional culture. Hum Reprod. 2013; 28(8): 2187-2200.
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31. Combelles CMH, Gupta S, Agarwal A. Could oxidative s tress influence the in-vitro maturation of oocytes? Reprod Biomed Online. 2009; 8(6): 864-880.
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32. Huang H, He X. Microscale materials and devices for cell vitrification by vitrification. In: He X, Bischof JC, editors. Multiscale technologies for cryomedicine-implementation from nano to macroscale. Singapore: World Scientific; 2016; 101-124.
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33. Huang H, Choi JK, Rao W, Zhao S, Agarwal P, Zhao G, et al. Alginate hydrogel microencapsulation inhibits devitrification and enables large-volume low-CPA cell vitrification. Adv Funct Mater. 2015; 25(44): 6839-6850.
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34. Zhang W, Liu Y, An Z, Huang D, Qi Y, Zhang Y. Mediating effect of ROS on mtDNA damage and low ATP content induced by arsenic trioxide in mouse oocytes. Toxicol In Vitro. 2011; 25(4): 979-984.
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35. Jin SY, Lei L, Shikanov A, Shea LD, Woodruff TK. A novel two-s tep s trategy for in vitro culture of early-s tage ovarian follicles in the mouse. Fertil S teril. 2010; 93(8): 2633-2639.
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36. Roy SK, Wang C, Mukherjee A, Chakraborty P. In vitro culture of fetal ovaries: a model to s tudy factors regulating early follicular development. Methods Mol Biol. 2012; 825: 151-171.
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37. Jalili C, Hemmatabadi FK, Mansouri K, Bakhtiyari M. Effects of sodium alginate capsules as 3D scaffolds on hormones and genes expression in preantral follicles of mice compared to 2D medium: an experimental s tudy. Int J Reprod Bio Med. 2020; 18(7): 517-525.
37
ORIGINAL_ARTICLE
Influence of Autologous In Vitro Activation of Ovaries by Stem Cells and Growth Factors on Endocrine and Reproductive Function of Patients with Ovarian Insufficiency-A Clinical Trial Study
Background: Premature ovarian failure (POF) can be found in 1% of women at the age of 35-40, mostly due to unknown causes. PI3K-Akt signaling is associated with both ovarian function and growth of primordial follicles. In this study, we examined the effects of autologous in vitro ovarian activation with stem cells and autologous growth factors on reproductive and endocrine function in patients with ovarian impairment. Materials and Methods: The longitudinal prospective observational study included 50 patients (between 30 and 50 years) with a diagnosis of POF and infertility. This multicenter study was performed at Jevremova Special Hospital in Belgrade, Saint James Hospital (Malta), and Remedica Skoplje Hospital, between 2015 and 2018. All patients went through numerous laboratory testings, including hormonal status. The autologous bone marrow mesenchymal stem cells (BMSCs) and growth factors were used in combination for activation of ovarian tissue before its re-transplantation. The software package SPSS 20.0 was used for statistical analysis of the results. Results: Differences in follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (PG) hormone concentrations before and after 3, 6, and 12 months post-transplantation were tested in correlation with the volume of transplanted ovarian tissue. A significant correlation (P=0.029) was found between the change in E2 level after 3 months and the volume of re-transplanted tissues. Also after re-transplantation, 64% of the patients had follicles resulting in aspiration of oocytes in 25% of positive women with follicles. Conclusion: The SEGOVA method could potentially solve many human reproductive problems in the future due to the large number of patients diagnosed with POF, as well asthe possibility of delaying menopause, thus improving the quality of life and general health (Registration number: NCT04009473).
https://www.ijfs.ir/article_241306_2cfc585c48a3548e33e14a7ac58691ca.pdf
2021-07-01
178
188
10.22074/ijfs.2020.134678
Growth factors
Ovarian
Premature Ovarian Failure
Stem cells
Suada
Tinjić
drtinjic@ginekologija.ba
1
Department of Gynecology, Polyclinic, Korak do Života, Tuzla, Bosnia and Herzegovina
LEAD_AUTHOR
Džihan
Abazović
adzihan@gmail.com
2
Department of Regenerative Medicine, Remedika Hospital, Belgrade, Serbia
AUTHOR
Dušica
Ljubić
pakoliliteo@yahoo.com
3
Department of Gynecology, MediGroup Hospital, Belgrade, Serbia
AUTHOR
Danilo
Vojvodić
vojvodic.danilo@gmail.com
4
Faculty of Medicine, Military Medical School, Belgrade, Serbia
AUTHOR
Tatjana
Božanović
tab145712@yahoo.com
5
Department of Gynecology and Obstetrics, Medical School, University of Belgrade, Belgrade, Serbia
AUTHOR
Mirza
Ibrišimović
mirza.ibrisimovic@ssst.edu.ba
6
Department of Gynecology, Polyclinic, Korak do Života, Tuzla, Bosnia and Herzegovina
AUTHOR
Sergije
Marković
sergije.markovic@untz.ba
7
Department of Histology, Medical School, University of Tuzla, Tuzla, Bosnia and Herzegovina
AUTHOR
Aleksandar
Ljubić
aleksljubicpriv@gmail.com
8
Department of Gynecology, Dubrovnik International University, Dubrovnik, Croatia
AUTHOR
1. Broekmans FJ, Knauff EAH, te Velde ER, Macklon NS, Fauser BC. Female reproductive ageing: current knowledge and future trends. Trends Endocrinol Metab. 2007; 18(2): 58-65.
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2. Silva JRV, van den Hurk R, van Tol HTA, Roelen BAJ, Figueiredo JR. Expression of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and BMP receptors in the ovaries of goats. Mol Reprod Dev. 2005; 70(1): 11-19.
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3. Dewailly D, Robin G, Peigne M, Decanter C, Pigny P, Catteau-Jonard S. Interactions between androgens, FSH, anti-Müllerian hormone and es tradiol during folliculogenesis in the human normal and polycys tic ovary. Hum Reprod Update. 2016; 22(6): 709-724.
3
4. Carrell DT, Peterson CM. Reproductive endocrinology and infertility. New York: Springer; 2010; 345.
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5. Qin Y, Zhao H, Xu J, Shi Y, Li Z, Qiao J, et al. Association of 8q22. 3 locus in Chinese Han with idiopathic premature ovarian failure (POF). Hum Mol Genet. 2011; 21(2): 430-436.
5
6. Tinjić S, Abazović Dž, Ljubić D, Vujović S, Vojvodić D, Božanović T, et al. Ovarian rejuvenation. Donald School J Ultrasound Obs te Gynecol. 2019; 13(2): 64-68.
6
7. Ljubić A, Abazović D, Vučetić D, Ljubić D, Pejović T, Božanović T. Case report autologous ovarian in vitro activation with ultrasound-guided orthotopic re-transplantation. Am J Clin Exp Obs tet Gynecol. 2017; 4(5): 51-57.
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8. He Y, Chen D, Yang L, Hou Q, Ma H, Xu X. The therapeutic potential of bone marrow mesenchymal s tem cells in premature ovarian failure. S tem Cell Res Ther. 2018; 9(1): 263.
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9. Evers JLH. Female subfertility. Lancet. 2002; 360(9327): 151-159.
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10. Kaspers GJ, Veerman AJ, Popp-Snijders C, Lomecky M, Van Zantwijk CH, Swinkels LM, et al. Comparison of the antileukemic activity in vitro of dexamethasone and prednisolone in childhood acute lymphoblas tic leukemia. Med Pediatr Oncol. 1996; 27(2): 114-121.
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11. Labarta E, de Los Santos MJ, Escriba MJ, Pellicer A, Herraiz S. Mitohondria as a oocyte rejuvenation. Fertil S teril. 2019; 111(2): 219-226.
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12. Hayashi K, Hikabe O, Obata Y, Hirao Y. Recons titution of mouse oogenesis in a dish from pluripotent s tem cells. Nat Protocol. 2017; 12(9): 1733-1744.
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13. Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, et al. Recons titution in vitro of the entire cycle of the mouse female germ line. Nature. 2016; 539(7628): 299-303.
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14. Morohaku K, Tanimoto R, Sasaki K, Kawahara-Miki R, Kono T, Hayashi K, et al. Complete in vitro generation of fertile oocytes from mouse primordial germ cells. Proc Natl Acad Sci USA. 2016; 113(32): 9021-9026.
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15. Friedens tein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow os teogenic s tem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet. 1987; 20(3): 263-272.
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16. Liu T, Huang Y, Guo L, Cheng W, Zou G. CD44+/CD105+ human amniotic fluid mesenchymal s tem cells survive and proliferate in the ovary long-term in a mouse model of chemotherapy-induced premature ovarian failure. Int J Med Sci. 2012; 9(7): 592-602.
16
17. Xiao GY, Liu IH, Cheng CC, Chang CC, Lee YH, Cheng WTK, Wu SC. Amniotic fluid s tem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy. PLoS One. 2014; 9(9): e106538.
17
18. Su J, Ding L, Cheng J, Yang J, Li X, Yan G, et al. Transplantation of adipose-derived s tem cells combined with collagen scaffolds res tores ovarian function in a rat model of premature ovarian insufficiency. Hum Reprod. 2016; 31: 1075-1086.
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19. Wang Z, Wang Y, Yang T, Li J, Yang X. S tudy of the reparative effects of mens trual-derived s tem cells on premature ovarian failure in mice. S tem Cell Res Ther. 2017; 8(1): 11.
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20. Lai D, Wang F, Yao X, Zhang Q, Wu X, Xiang C. Human endometrial mesenchymal s tem cells res tore ovarian function through improving the renewal of germline s tem cells in a mouse model of premature ovarian failure. J Transl Med. 2015; 13(1): 155.
20
21. Zhu SF, Hu HB, Xu HY, Fu XF, Peng DX, Su WY, et al. Human umbilical cord mesenchymal s tem cell transplantation res tores damaged ovaries. J Cell Mol Med. 2015; 19: 2108-2117.
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22. Elfayomy AK, Almasry SM, El-Tarhouny SA, Eldomiaty MA. Human umbilical cord blood-mesenchymal s tem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: possible direct and indirect effects. Tissue Cell. 2016; 48(4): 370-382.
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23. Adler DS, Lazarus H, Nair R, Goldberg JL, Greco NJ, Lassar T, et al. Safety and efficacy of bone marrow-derived autologous CD133+ s tem cell therapy. Front Biosci (Elite Ed). 2011; 3: 506-514.
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24. Bongiovanni D, Bassetti B, Gambini E, Gaipa G, Frati G, Achilli F, et al. The CD133+ cell as advanced medicinal product for myocardial and limb ischemia. S tem Cells Dev. 2014; 23(20) : 2403-2421.
24
25. Ahmadi H, Baharvand H, Ashtiani SK, Soleimani M, Sadeghian H, Ardekani JM, et al. Safety analysis and improved cardiac function following local autologous transplantation of CD133(+) enriched bone marrow cells after myocardial infarction. Curr Neurovasc Res. 2007; 4(3): 153-160.
25
26. Alvero R. Editorial: Challenging topics in reproductive endocrinology. Curr Opin Obs tet Gynecol. 2020; 32(5): 359-360.
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27. Elkhenany HA, Szojka ARA, Mulet-Sierra A, Liang Y, Kunze M, Lan X, et al. Bone marrow mesenchymal s tem cells-derived tissues are mechanically superior to meniscus cells. Tissue Eng Part A. 2020 (ahead of print).
27
28. Kasapoğlu I, Seli E. Mitochondrial dysfunction and ovarian aging. Endocrinology. 2020; 161(2): bqaa001.
28
29. Petryk N, Petryk M. Ovarian rejuvenation through platelet-rich autologous plasma (PRP)—a chance to have a baby without donor eggs, improving the life quality of women suffering from early menopause without synthetic hormonal treatment. Reprod Sci. 2020; 27(11): 1975-1982.
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30. Kawamura K, Kawamura N, Hsueh AJW. Activation of dormant follicles: a new treatment for premature ovarian failure? Curr Opin Obs tet Gynecol. 2016; 28(3): 217-222.
30
ORIGINAL_ARTICLE
Infertility Stigma: A Qualitative Study on Feelings and Experiences of Infertile Women
Background: Infertility stigma is a phenomenon associated with various psychological and social tensions especially for women. The stigma is associated with a feeling of shame and secrecy. The present study was aimed to explore the concept of infertility stigma based on the experiences and perceptions of infertile women. Materials and Methods: This qualitative conventional content analysis study was conducted in Isfahan Fertility and Infertility Center, Iran. Data were collected through in-depth interviews with 17 women who had primary infertility. All the interviews were recorded, transcribed and analyzed according to the steps suggested by Graneheim and Lundman. The Standards for Reporting Qualitative Research (SRQR) checklist was followed for this research. Results: Eight hundred thirty-six initial codes were extracted from the interviews and divided into 25 sub-categories, 10 categories, and four themes. The themes included “stigma profile, self-stigma, defensive mechanism and balancing”. Stigma profile was perceived in the form of verbal, social and same sex stigma. Self-stigma was experienced as negative feelings and devaluation. Defensive mechanism was formed from three categories of escaping from the stigma, acceptance and infertility behind the mask. Two categories; empowered women and pressure levers, created a balancing theme against the infertility stigma. Conclusion: Infertile women face social and self-stigma which threatenstheir psychosocial wellbeing and self-esteem. They use defensive response mechanisms and social support to mitigate these effects. Education focused on coping strategies might be helpful against infertility stigma.
https://www.ijfs.ir/article_243834_5626f46ff402d0bc38074a5f6c511ece.pdf
2021-07-01
189
196
10.22074/ijfs.2021.139093.1039
Female infertility
infertility
Stigma
qualitative study
Mahboubeh
Taebi
m_taebi@nm.mui.ac.ir
1
Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Nourossadat
Kariman
n_kariman@sbmu.ac.ir
2
Department of Midwifery and Reproductive Health, Midwifery and Reproductive Health Research Center, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Ali
Montazeri
montazeri@aecr.ac.ir
3
Health Metrics Research Centre, Iranian Ins titute for Health Sciences Research, ACECR, Tehran, Iran
AUTHOR
Hamid
Alavi Majd
alavimajdd@gmail.com
4
Department of Bios tatis tics, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
1. WHO. Infertility is a global public health issue. Available from: https://www.who.int/reproductivehealth/topics/infertility/perspective/en/. (26 Nov 2019).
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2. Wasilewski T, Łukaszewicz-Zając M, Wasilewska J, Mroczko B. Biochemis try of infertility. Clin Chim Acta. 2020; 508: 185-190.
2
3. Datta J, Palmer MJ, Tanton C, Gibson LJ, Jones KG, Macdowall W, et al. Prevalence of infertility and help seeking among 15 000 women and men. Hum Reprod. 2016; 31(9): 2108-2118.
3
4. Slade P, O'Neill C, Simpson AJ, Lashen H. The relationship between perceived s tigma, disclosure patterns, support and dis tress in new attendees at an infertility clinic. Hum Reprod. 2007; 22(8): 2309-2317.
4
5. Hasanpoor-Azghady SB, Simbar M, Vedadhir AA, Azin SA, Amiri-Farahani L. The social cons truction of infertility among iranian infertile women: a qualitative s tudy. J Reprod Infertil. 2019; 20(3): 178-190.
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6. Fu B, Qin N, Cheng L, Tang G, Cao Y, Yan C, et al. Development and validation of an infertility s tigma scale for chinese women. J Psychosom Res. 2015; 79(1): 69-75.
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7. Karaca A, Unsal G. Psychosocial problems and coping s trategies among Turkish women with infertility. Asian Nurs Res (Korean Soc Nurs Sci). 2015; 9(3): 243-250.
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8. Greil AL, Slauson-Blevins K, McQuillan J. The experience of infertility: a review of recent literature. Sociol Health Illn. 2010; 32(1): 140-162.
8
9. Fledderjohann JJ. 'Zero is not good for me': implications of infertility in Ghana. Hum Reprod. 2012; 27(5): 1383-1390.
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10. Pacheco Palha A, Lourenco MF. Psychological and cross-cultural aspects of infertility and human sexuality. Adv Psychosom Med. 2011; 31: 164-183.
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11. Goffman E. S tigma: notes on the management of spoiled identity. New York: Simon and Schus ter; 2009.
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12. Carter J, Applegarth L, Josephs L, Grill E, Baser RE, Rosenwaks Z. A cross-sectional cohort s tudy of infertile women awaiting oocyte donation: the emotional, sexual, and quality-of-life impact. Fertil S teril. 2011; 95(2): 711-716. e1.
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13. Donkor ES, Sandall J. The impact of perceived s tigma and mediating social factors on infertility-related s tress among women seeking infertility treatment in Southern Ghana. Soc Sci Med. 2007; 65(8): 1683-1694.
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14. S ternke EA, Abrahamson K. Perceptions of women with infertility on s tigma and disability. Sex Disabil. 2015; 33(1): 3-17.
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15. O'Brien BC, Harris IB, Beckman TJ, Reed DA, Cook DA. S tandards for reporting qualitative research: a synthesis of recommendations. Acad Med. 2014; 89(9): 1245-1251.
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16. Guba E, Lincoln Y. Effective evaluation: improving the usefulness of evaluation results throgh responsive and naturalis tic approaches. 1s t ed. San francisco: CA: Jossey-Bass; 1981.
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17. O’Connor C, Joffe H. Intercoder reliability in qualitative research: debates and practical guidelines. Int J Qual Methods. 2020; 19: 1609406919899220.
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18. LoBiondo-Wood G, Haber J. Nursing research: methods and critical appraisal for evidence-based practice. 6th ed. S t. Louis: Mosby Elsevier; 2006.
18
19. Dyer SJ, Abrahams N, Hoffman M, van der Spuy ZM. Men leave me as I cannot have children: women's experiences with involuntary childlessness. Hum Reprod. 2002; 17(6): 1663-1668.
19
20. Kearney AL, White KM. Examining the psychosocial determinants of women's decisions to delay childbearing. Hum Reprod. 2016; 31(8): 1776-1787.
20
21. Luk BH, Loke AY. The impact of infertility on the psychological well-being, marital relationships, sexual relationships, and quality of life of couples: a sys tematic review. J Sex Marital Ther. 2015; 41(6): 610-625.
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22. Mumtaz Z, Shahid U, Levay A. Unders tanding the impact of gendered roles on the experiences of infertility amongs t men and women in Punjab. Reprod Health. 2013; 10: 3.
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23. Diamond R, Meyers M, Kezur D, Scharf CN, Weinshel M. Couple therapy for infertility. Newyork: Guilford; 1991.
23
24. Dimka RA, Dein SL. The work of a woman is to give birth to children: cultural cons tructions of infertility in Nigeria. Afr J Reprod Health. 2013; 17(2): 102-117.
24
25. Younesi SJ, Akbari-Zardkhaneh S, Behjati Ardakani Z. Evaluating s tigma among infertile men and women in Iran. J Reprod Infertil. 2006; 6(5): 531-546.
25
26. Alhassan A, Ziblim AR, Muntaka S. A survey on depression among infertile women in Ghana. BMC Women's Health. 2014; 14(1): 42.
26
27. Fahami F, Quchani SH, Ehsanpour S, Boroujeni AZ. Lived experience of infertile men with male infertility cause. Iran J Nurs Midwifery Res. 2010; 15 Suppl 1: 265-271.
27
28. Gonzalez LO. Infertility as a transformational process: a framework for psychotherapeutic support of infertile women. Issues Ment Health Nurs. 2000; 21(6): 619-633.
28
29. Musa R, Ramli R, Yazmie AWA, Khadijah MBS, Hayati MY, Midin M, et al. A preliminary s tudy of the psychological differences in infertile couples and their relation to the coping s tyles. Compr Psychiatry. 2014; 55 Suppl 1: S65-S69.
29
30. Cizmeli C, Lobel M, Franasiak J, Pas tore LM. Levels and associations among self-es teem, fertility dis tress, coping, and reaction to potentially being a genetic carrier in women with diminished ovarian reserve. Fertil S teril. 2013; 99(7): 2037-2044. e3.
30
31. Anokye R, Acheampong E, Mprah WK, Ope JO, Barivure TN. Psychosocial effects of infertility among couples attending S t. Michael's Hospital, Jachie-Pramso in the Ashanti Region of Ghana. BMC Res Notes. 2017; 10(1): 690.
31
32. Kato A, Fujimaki Y, Fujimori S, Isogawa A, Onishi Y, Suzuki R, et al. Association between self-s tigma and self-care behaviors in patients with type 2 diabetes: a cross-sectional s tudy. BMJ Open Diabetes Res Care. 2016; 4(1): e000156.
32
33. Ceballo R, Graham ET, Hart J. Silent and infertile: an intersectional analysis of the experiences of socioeconomically diverse African American women with infertility. Psychol Women Q. 2015; 39(4): 497-511.
33
34. Ranjbar F, Behboodi-Moghadam Z, Borimnejad L, Ghaffari SR, Akhondi MM. Experiences of infertile women seeking assis ted pregnancy in Iran: a qualitative s tudy. J Reprod Infertil. 2015; 16(4): 221-228.
34
35. Ried K, Alfred A. Quality of life, coping s trategies and support needs of women seeking Traditional Chinese Medicine for infertility and viable pregnancy in Aus tralia: a mixed methods approach. BMC Women's Health. 2013; 13: 17.
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36. Kabeer N. Resources, agency, achievements: Reflections on the measurement of women's empowerment. Dev Change. 1999; 30(3): 435-464.
36
37. Kiesswetter M, Marsoner H, Luehwink A, Fis tarol M, Mahlknecht A, Duschek S. Impairments in life satisfaction in infertility: Associations with perceived s tress, affectivity, partnership quality, social support and the desire to have a child. Behav Med. 2020; 46(2): 130-141.
37
38. Dennis CL. Peer support within a health care context: a concept analysis. Int J Nurs S tud. 2003; 40(3): 321-332.
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39. Alavi NM, Alami L, Taefi S, Gharabagh GS. Factor analysis of self-treatment in diabetes mellitus: a cross-sectional s tudy. BMC Public Health. 2011; 11: 761.
39
ORIGINAL_ARTICLE
Follicular Fluid Zinc Level and Oocyte Maturity and Embryo Quality in Women with Polycystic Ovary Syndrome
Background: Polycystic ovary syndrome (PCOS) is considered to be one of the most common endocrine disorders in women of reproductive age. Zinc, a vital trace element in the body, plays a key role in maintaining health, especially due to its antioxidant role. On the other hand, lack of antioxidants and oxidative stress can adversely affect oocytes quality and consequently fertility rate. The available studiesthat report the effect of follicular fluid (FF) zinc in terms of the number and quality of the oocytes in infertile women with PCOS, are few and not consistent. We decided to investigate this issue. Materials and Methods: In this cross-sectional study, from the women with PCOS referring to Omolbanin Hospital, Dezful, Iran (February to December 2019), a total of 90 samples (follicular fluid, oocytes, and embryos) were collected from those who had undergone in vitro fertilization (IVF). To measure zinc level in follicular fluid, high performance liquid chromatograpy (HPLC) was utilized. Also, oocytes maturity and embryos quality evaluation was performed using inverted optical microscopy. One-way ANOVA and Fisher’s least significant difference (LSD) were used for data analysis. Results: The amount of FF zinc was not associated with any significant differences in the number of oocytes and metaphase I (MI) and germinal vesicle (GV) oocytes, but a significant decrease was observed in the number of metaphase II (MII) oocytes at zinc values lessthan 35 μg/dL. The FF zinc levels lessthan 35 μg/dL were also significantlyassociated with decreased embryo quality. Conclusion: A significant relationship was found between the level of FF zinc and the quality and the number of oocytes taken from the ovaries of infertile patients with PCOS history who were candidates for IVF treatment as well as the number of high quality embryos.
https://www.ijfs.ir/article_241407_ae2eb551d0cb53f64ea18625eb662ccc.pdf
2021-07-01
197
201
10.22074/ijfs.2021.135426.1006
embryo
Oocyte
Polycys tic Ovary Syndrome
Zinc
Sima
Janati
sjanati@ymail.com
1
Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran
AUTHOR
Mohammad Amin
Behmanesh
behmanesh.ma@yahoo.com
2
Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
AUTHOR
Hosein
Najafzadehvarzi
najafzadehvarzi@yahoo.com
3
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
AUTHOR
Zahra
Akhundzadeh
zahra.akhundzade@yahoo.com
4
School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
AUTHOR
Seyyedeh Mahsa
Poormoosavi
m.poormoosavi@ymail.com
5
Department of Histology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran
LEAD_AUTHOR
1. Özer A, Bakacak M, Kıran H, Ercan Ö, Kös tü B, Kanat-Pektaş M, et al. Increased oxidative s tress is associated with insulin resis tance and infertility in polycys tic ovary syndrome. Ginekologia Polska. 2016; 87(11): 733-738.
1
2. Hwang KR, Choi YM, Kim JJ, Chae SJ, Park KE, Jeon HW, et al. Effects of insulin-sensitizing agents and insulin resis tance in women with polycys tic ovary syndrome. Clin Exp Reprod Med. 2013; 40(2): 100-105.
2
3. Álvarez-Blasco F, Botella-Carretero JI, San Millán JL, Escobar-Morreale HF. Prevalence and characteris tics of the polycys tic ovary syndrome in overweight and obese women. Arch Intern Med. 2006; 166(19): 2081-2086.
3
4. Zhang Y, Liu L, Yin TL, Yang J, Xiong CL. Follicular metabolic changes and effects on oocyte quality in polycys tic ovary syndrome patients. Oncotarget. 2017; 8(46): 80472-80480.
4
5. Dumesic DA, Lobo RA. Cancer risk and PCOS. S teroids. 2013; 78(8): 782-785.
5
6. Afiat M, Khadem N, Nayeri E, Jalali R, Akhlaghi S, Akhgari E, et al. Comparison of oocyte and embryo quality in women with polycys tic ovary syndrome and the control group candidate for in vitro fertilization and intracytoplasmic sperm injection. Int J Women's Health Reprod Sci. 2021; 9(3): 1-7.
6
7. Arya BK, Haq AU, Chaudhury K. Oocyte quality reflected by follicular fluid analysis in poly cys tic ovary syndrome (PCOS): a hypothesis based on intermediates of energy metabolism. Med Hypotheses. 2012; 78(4): 475-478.
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8. Singh AK, Chattopadhyay R, Chakravarty B, Chaudhury K. Markers of oxidative s tress in follicular fluid of women with endometriosis and tubal infertility undergoing IVF. Reprod Toxicol. 2013; 42: 116-124.
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9. Kim YS, Kim MS, Lee SH, Choi BC, Lim JM, Cha KY, et al. Proteomic analysis of recurrent spontaneous abortion: identification of an inadequately expressed set of proteins in human follicular fluid. Proteomics. 2006; 6(11): 3445-3454.
9
10. Tolunay HE, Şükür YE, Ozkavukcu S, Seval MM, Ateş C, Türksoy VA, et al. Heavy metal and trace element concentrations in blood and follicular fluid affect ART outcome. Eur J Obs tet Gynecol Reprod Biol. 2016; 198: 73-77.
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11. Prasad AS. Zinc: mechanisms of hos t defense. J Nutr. 2007; 137(5): 1345-1349.
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12. Bedwal RS, Bahuguna A. Zinc, copper and selenium in reproduction. Experientia. 1994; 50(7): 626-640.
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13. Ménézo Y, Pluntz L, Chouteau J, Gurgan T, Demirol A, Dalleac A, et al. Zinc concentrations in serum and follicular fluid during ovarian s timulation and expression of Zn2+ transporters in human oocytes and cumulus cells. Reprod Biomed Online. 2011; 22(6): 647-652.
13
14. Özkaya MO, Nazıroğlu M, Barak C, Berkkanoglu M. Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF). Biol Trace Elem Res. 2011; 139(1): 1-9.
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15. Poormoosavi SM, Behmanesh MA, Janati S, Najafzadehvarzi H. Level of bisphenol a in follicular fluid and serum and oocyte morphology in patients undergoing IVF treatment. J Family Reprod Health. 2019; 13(3): 154-159.
15
16. Bahadori MH, Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF. Level of vitamin e in follicular fluid and serum and oocyte morphology and embryo quality in patients undergoing IVF treatment. J Family Reprod Health. 2017; 11(2): 74-81.
16
17. Scott L, Alvero R, Leondires M, Miller B. The morphology of human pronuclear embryos is positively related to blas tocys t development and implantation. Hum Reprod. 2000; 15(11): 2394-2403.
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18. Sparks A. An atlas of human gametes and conceptuses: an illus trated reference for assis ted reproductive technology. In: Veeck LL, editor.1s t ed. CRC Press; 1999.
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19. Boland M, Lonergan P, O'callaghan D. Effect of nutrition on endocrine parameters, ovarian physiology, and oocyte and embryo development. Theriogenology. 2001; 55(6): 1323-1340.
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20. Kurdoglu Z, Kurdoglu M, Demir H, Sahin HG. Serum trace elements and heavy metals in polycys tic ovary syndrome. Hum Exp Toxicol. 2012; 31(5): 452-456.
20
21. Sun Y, Lin Y, Niu M, Kang Y, Du S, Zheng B. Follicular fluid concentrations of zinc and copper are positively associated with in vitro fertilization outcomes. Int J Clin Exp Med. 2017; 10(2): 3547-3553.
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22. Seleem AK, El Refaeey AA, Shaalan D, Sherbiny Y, Badawy A. Superoxide dismutase in polycys tic ovary syndrome patients undergoing intracytoplasmic sperm injection. J Assis t Reprod Genet. 2014; 31(4): 499-504.
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23. Pang W, Leng X, Lu H, Yang H, Song N, Tan L, et al. Depletion of intracellular zinc induces apoptosis of cultured hippocampal neurons through suppression of ERK signaling pathway and activation of caspase-3. Neurosci Lett. 2013; 552: 140-145.
23
24. Kumar S, Mishra V, Thaker R, Gor M, Perumal S, Joshi P, et al. Role of environmental factors & oxidative s tress with respect to in vitro fertilization outcome. Indian J Med Res. 2018; 148 Suppl 1: S125-S133.
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25. Jeon Y, Yoon JD, Cai L, Hwang SU, Kim E, Zheng Z, et al. Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure. Mol Med Rep. 2015; 12(4): 5973-5982.
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26. Tian X, Diaz FJ. Acute dietary zinc deficiency before conception compromises oocyte epigenetic programming and disrupts embryonic development. Dev Biol. 2013; 376(1): 51-61.
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27. Tian XI, Diaz FJ. Zinc deficiency during oocyte maturation causes defects in preimplantation embryonic development. Biol Reprod. 2012; 87(Suppl 1): 199.
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28. Özkaya MO, Nazıroğlu M. Multivitamin and mineral supplementation modulates oxidative s tress and antioxidant vitamin levels in serum and follicular fluid of women undergoing in vitro fertilization. Fertil S teril. 2010; 94(6): 2465-2466.
28
ORIGINAL_ARTICLE
Clinical and Molecular Effects of GnRH Agonist and Antagonist on The Cumulus Cells in The In Vitro Fertilization Cycle
Background: Gonadotropin-releasing hormone (GnRH) analogues have been extensively utilized in the ovarian s timulationcycle for suppression of endogenous rapid enhancement of luteinizing hormone (LH surge). Exclusive propertiesand functional mechanisms of GnRH analogues in in vitro fertilization (IVF) cycles are clearly described. This s tudy wasperformed to evaluate clinical and molecular impacts of the GnRH agonis t and antagonis t protocols in IVF cycles. Forthis purpose, gene expression of cumulus cells (CCs) as well as clinical and embryological parameters were evaluatedand compared between two groups (GnRH agonis t and antagonis t) during the IVF cycle. Materials and Methods: Twenty-one infertile individuals were enrolled in this s tudy. Subjects wereselected from two groups of GnRH agonis t (n=10) treated patients and GnRH antagonis t (n=11) treated individuals.The defined clinical embryological parameters were compared between the two groups. Expression of BAX, BCL-2,SURVIVIN, ALCAM, and VCAN genes were assessed in the CCs of the participants using the real-time polymerasechain reaction (PCR) technique. Results: The mean number of cumulus oocyte complex (COC), percentage of metaphase II (MII) oocytes, grade Aembryo and clinical parameters did not show noticeable differences between the two groups. BAX gene expression inthe CCs of the group treated with GnRH agonis t was remarkably higher than those received GnRH antagonis t treatment(P<0.001). The mRNA expression of BCL-2 and ALCM genes were considerably greater in the CCs of patientswho underwent antagonis t protocol in comparison to the group that received agonis t protocol (P<0.001). Conclusion: Despite no considerable difference in the oocyte quality, embryo development, and clinical outcomes betweenthe group treated with GnRH agonis t and the one treated with antagonis t protocol, the GnRH antagonis t protocolwas slightly more favorable. However, further clinical s tudies using molecular assessments are required to elucidatethis controversial subject.
https://www.ijfs.ir/article_239411_852c657994edf7b7589e4bb3c6e6defe.pdf
2021-07-01
202
209
10.22074/ijfs.2020.136161.1012
Apoptosis
Cumulus Cells
GnRH Agonist
Saeed
Azizollahi
saeedazizollahi@yahoo.com
1
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Maryam
Bagheri
m_bagheri@yahoo.com
2
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Fedyeh
Haghollahi
fedyeh_hagh@yahoo.com
3
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
Seyyede Momeneh
Mohamadi
mohamady3@gmail.com
4
Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
AUTHOR
Batool
Hosein Rashidi
bhrashidi@gmail.com
5
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
1. Xia L, Tian L, Tan J, Zhang S, Wu QF. GnRH antagonis t versus modified prolonged GnRH agonis t protocol in polycys tic ovary syndrome (pcos): analysis using propensity score matching. 2020. Available from: https://www.researchsquare.com/article/rs-63654/ v1. (31 Aug 2020)
1
2. Wu HM, Chang HM, Leung PC. Gonadotropin-releasing hormone analogs: mechanisms of action and clinical applications in female reproduction. Front Neuroendocrinol. 2020; 60: 100876.
2
3. Lin MH, Wu FSY, Hwu YM, Lee RKK, Li RS, Li SH. Dual trigger with gonadotropin releasing hormone agonis t and human chorionic gonadotropin significantly improves live birth rate for women with diminished ovarian reserve. Reprod Biol Endocrinol. 2019; 17: 7.
3
4. Chen Q, Yu F, Li Y, Zhang AJ, Zhu XB. Comparative proteomics reveal negative effects of gonadotropin-releasing hormone agonis t and antagonis t on human endometrium. Drug Des Devel Ther. 2019; 13: 1855-1863.
4
5. Güzel Y. Follicle-s timulating hormone induced in vitro growth of small antral follicles are not affected by gonadotropin-releasing hormone agonis t or antagonis t treatment in mice. Eur Arch Med Res. 2019; 35(4): 211-214.
5
6. Tabibnejad N, Sheikhha MH, Ghasemi N, Fesahat F, Soleimani M, Aflatoonian A. Association between early embryo morphokinetics plus cumulus cell gene expression and assis ted reproduction outcomes in polycys tic ovary syndrome women. Reprod Biomed Online. 2019; 38(2): 139-151.
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7. Li Q, McKenzie LJ, Matzuk MM. Revisiting oocyte–somatic cell interactions: in search of novel intrafollicular predictors and regulators of oocyte developmental competence. Mol Hum Reprod. 2008; 14(12): 673-678.
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8. Adriaenssens T, Segers I, Wathlet S, Smitz J. The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blas tocys t formation. J Assis t Reprod Genet. 2011; 28(1): 31-40.
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9. Nagyova E, Salus tri A, Nemcova L, Scsukova S, Kalous J, Camaioni A. Versican G1 fragment es tablishes a s trongly s tabilized interaction with hyaluronan-rich expanding matrix during oocyte maturation. Int J Mol Sci. 2020; 21(7): 2267.
9
10. Wei LL, Pan YS, Tang Q, Yang ZJ, Song WQ, Gao YF, et al. Decreased ALCAM expression and promoter hypermethylation is associated with preeclampsia. Hypertens Res. 2019; 43(1): 13-22.
10
11. Adriaenssens T, Van Vaerenbergh I, Coucke W, Segers I, Verheyen G, Anckaert E, et al. Cumulus-corona gene expression analysis combined with morphological embryo scoring in single embryo transfer cycles increases live birth after fresh transfer and decreases time to pregnancy. J Assis t Reprod Genet. 2019; 36(3): 433-443.
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12. Jančar N, Kopitar AN, Ihan A, Klun IV, Bokal EV. Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blas tocys t development. J Assis t Reprod Genet. 2007; 24(2-3): 91-97.
12
13. Chermuła B, Kranc W, Jopek K, Budna-Tukan J, Hutchings G, Dompe C, et al. Human cumulus cells in long-term in vitro culture reflect differential expression profile of genes responsible for planned cell death and aging—a s tudy of new molecular markers. Cells. 2020; 9(5): 1265.
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14. Faramarzi A, Khalili MA, Jahromi MG. Is there any correlation between apoptotic genes expression in cumulus cells with embryo morphokinetics? Mol Biol Rep. 2019; 46: 3663-3670.
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15. Johnson AL, Langer JS, Bridgham JT. Survivin as a cell cycle-related and antiapoptotic protein in granulosa cells. Endocrinology. 2002; 143(9): 3405-3413.
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16. Jiang Z, Hu M, Wang Z, Huang L, Lin F, Qi S, et al. Survivin is essential for fertile egg production and female fertility in mice. Cell Death Dis. 2014; 5(3): e1154.
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17. Demiray SB, Goker ENT, Tavmergen E, Yilmaz O, Calimlioglu N, Soykam HO, et al. Differential gene expression analysis of human cumulus cells. Clin Exp Reprod Med. 2019; 46(2): 76-87.
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18. Hussein MR. Apoptosis in the ovary: molecular mechanisms. Hum Reprod Update. 2005; 11(2): 162-178.
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19. Chi XX, Zhang T, Chu XL, Zhen JL, Zhang DJ. The regulatory effect of Genis tein on granulosa cell in ovary of rat with PCOS through Bcl-2 and Bax signaling pathways. J Vet Med Sci. 2018; 80(8): 1348-1355.
19
20. Mathyk BA, Cetin BA, Vardagli D, Zengin E, Sofiyeva N, Irez T, et al. Comparison of antagonis t mild and long agonis t protocols in terms of follicular fluid total antioxidant capacity. Taiwan J Obs tet Gyne. 2018; 57(2): 194-199.
20
21. Lee SH, Oh HJ, Kim MJ, Lee BC. Exosomes derived from oviduct cells mediate the EGFR/MAPK signaling pathway in cumulus cells. J Cell Physiol. 2020; 235(2): 1386-1404.
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22. S tamperna K, Giannoulis T, Nanas I, Kalemkeridou M, Dadouli K, Moutou K, et al. Short term temperature elevation during IVM affects embryo yield and alters gene expression pattern in oocytes, cumulus cells and blas tocys ts in cattle. Theriogenology. 2020; 156: 36-45.
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23. Devjak R, Papler TB, Verdenik I, Tacer KF, Bokal EV. Embryo quality predictive models based on cumulus cells gene expression. Balkan J Med Genet. 2016; 19(1) :5-12.
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24. Clavero A, Cas tilla JA, Núñez AI, Garcı́a-Peña ML, Maldonado V, Fontes J, et al. Apoptosis in human granulosa cells after induction of ovulation in women participating in an intracytoplasmic sperm injection program. Eur J Obs tet Gynecol Reprod Biol. 2003; 110(2):181-185.
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25. Lee KS, Joo BS, Na YJ, Yoon MS, Choi OH, Kim WW. Clinical assis ted reproduction: cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF–ET. J Assis t Reprod Genet. 2001; 18(9): 490-498.
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26. Van Montfoort AP, Dumoulin JC, Kes ter AD, Evers JL. Early cleavage is a valuable addition to exis ting embryo selection parameters: a s tudy using single embryo transfers. Hum Reprod. 2004; 19(9): 2103-2108.
26
27. Varras M, Polonifi K, Mantzourani M, S tefanidis K, Papadopoulos Z, Akrivis C, et al. Expression of antiapoptosis gene survivin in luteinized ovarian granulosa cells of women undergoing IVF or ICSI and embryo transfer: clinical correlations. Reprod Biol Endocrinol. 2012; 10(1): 74.
27
28. Assou S, Haouzi D, Mahmoud K, Aouacheria A, Guillemin Y, Pantesco V, et al. A non-invasive tes t for assessing embryo potential by gene expression profiles of human cumulus cells: a proof of concept s tudy. Mol Hum Reprod. 2008; 14(12): 711-719.
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29. Geng Y, Xun Y, Hu S, Lai Q, Jin L. GnRH antagonis t versus follicular-phase single-dose GnRH agonis t protocol in patients of normal ovarian responses during controlled ovarian s timulation. Gynecol Endocrinol. 2019; 35(4): 309-313.
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30. Kara M, Aydin T, Aran T, Turktekin N, Ozdemir B. Comparison of GnRH agonis t and antagonis t protocols in normoresponder patients who had IVF-ICSI. Arch Gynecol Obs tet. 2013; 288(6): 1413-1416.
30
31. Prapas Y, Petousis S, Dagklis T, Panagiotidis Y, Papatheodorou A, Assunta I, et al. GnRH antagonis t versus long GnRH agonis t protocol in poor IVF responders: a randomized clinical trial. Eur J Obs tet Gynecol Reprod Biol. 2013; 166(1): 43-46.
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32. Takahashi K, Mukaida T, Tomiyama T, Goto T, Oka C. GnRH antagonis t improved blas tocys t quality and pregnancy outcome after multiple failures of IVF/ICSI–ET with a GnRH agonis t protocol. J Assis t Reprod Genet. 2004; 21(9): 317-322.
32
33. de Souza Jordão ÉV, Nakagawa HM, Es trela FS, de Morais RB, Gomes-Sobrinho DB, de Carvalho BR. Outcomes of GnRH agonis t and GnRH antagonis t regimens for IVF in women aged up to 40. Acta Med. 2016; 3(1): 46-49.
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34. Xiao Js, Su Cm, Zeng Xt. Comparisons of GnRH antagonis t versus GnRH agonis t protocol in supposed normal ovarian responders undergoing IVF: a sys tematic review and meta-analysis. PLoS One. 2014; 9(9): e106854.
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35. Adriaenssens T, Wathlet S, Segers I, Verheyen G, De Vos A, Van der Els t J, et al. Cumulus cell gene expression is associated with oocyte developmental quality and influenced by patient and treatment characteris tics. Hum Reprod. 2010; 25(5): 1259-1270.
35
36. Fujiwara H, Tatsumi K, Kosaka K, Sato Y, Higuchi T, Yoshioka S, et al. Human blas tocys ts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 2003; 88(7): 3437-3443.
36
37. Hernandez-Gonzalez I, Gonzalez-Robayna I, Shimada M, Wayne CM, Ochsner SA, White L, et al. Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune-related genes: does this expand their role in the ovulation process? Mol Endocrinol. 2006; 20(6): 1300-1321.
37
ORIGINAL_ARTICLE
Protective Effect of Aloe vera Gel against Cisplatin-Induced Testicular Damage, Sperm Alteration and Oxidative Stress in Rats
Background: Cisplatin is an effective antineoplastic drug that is used to treat varioustypes of cancers. However, it causes side effects on the male reproductive system. The present study aimed to investigate the possible protective effects of Aloe vera gel (known as an antioxidant plant) on cisplatin-induced changes in rat spermparameters, testicular structure, and oxidative stress markers. Materials and Methods: In this experimental study, forty-eight adult male rats were divided into 6 groups including: control, cisplatin (CIS), A. vera (AL), metformin (MET), cisplatin+A. vera (CIS-AL), and cisplatin+metformin (CIS-MET). Cisplatin was used intraperitoneally at a dose of 5 mg/kg on days 7, 14, 21, and 28 of the experiment. A. vera gel (400 mg/kg per day) and metformin (200 mg/kg per day) were administered orally for 35 days (started one week before the beginning of the experiment). Testes weight and dimensions, and morphometrical and histological alterations, activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GPx), serum testosterone concentration, lipid peroxidation level, and sperm parameters were examined. Results: Cisplatin caused a significant decrease (p <0.05) in relative weight and dimension of the testis, germinal epithelium thickness and diameter of seminiferoustubules, the numbers of testicular cells, and spermatogenesis indexes.The malondialdehyde (MDA) levels increased and antioxidant enzymes activities decreased in the CIS group compared to the control group (p <0.05). Additionally, sperm parameters (concentration, viability, motility, and normal morphology), and testosterone levels reduced significantly in cisplatin-treated rats (p <0.05). Also, cisplatin induced histopathological damages including disorganization, desquamation, atrophy, and vacuolation in the testis. However, administration of A. vera gel to cisplatin-treated rats attenuated the cisplatin-induced alterations, mitigated testicular oxidative stress and increased testosterone concentration. Conclusion: The results suggest that A. vera as a potential antioxidant plant and due to free radicals scavenging activities, has a protective effect against cisplatin-induced testicular alterations.
https://www.ijfs.ir/article_48026_d40ec39faa1d107c10f7e5ec620ce0e7.pdf
2021-07-01
210
218
10.22074/ijfs.2020.134691
Aloe vera
cisplatin
Oxidative stress
Rat
Testis
Naeem
Erfani Majd
naeemalbo@yahoo.com
1
Department of Basic Sciences, Histology Section, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
Mohammad Reza
Tabandeh
m.tabandeh@scu.ac.ir
2
Stem Cell and Transgenic Technology Research Center, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Shima
Hosseinifar
sh.hosseinifar@scu.ac.ir
3
Department of Basic Sciences, Histology Section, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Mahin
Sadeghi
sadeghimahin.86@gmail.com
4
Department of Basic Sciences, Histology Section, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
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2. Ijaz MU, Tahir A, Samad A, Ashraf A, Ameen M, Imran M, et al. Cas ticin alleviates tes ticular and spermatological damage induced by cisplatin in rats. Pak Vet J. 2020; 40 (2): 234-238.
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4. Azarbarz N, Shafiei Seifabadi Z, Moaiedi MZ, Mansouri E. Assessment of the effect of sodium hydrogen sulfide (hydrogen sulfide donor) on cisplatin-induced tes ticular toxicity in rats. Environ Sci Pollut Res Int. 2020; 27(8): 8119-8128.
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5. Almeer RS, Abdel Moneim AE. Evaluation of the protective effect of olive leaf extract on cisplatin-induced tes ticular damage in rats. Oxid Med Cell Longev. 2018: 8487248.
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35. Modaresi M, Khodaii H, Khodadadi A. The effects of Aloe vera extract on spermatogenesis and reproductive hormones in mice. J Anim Biol. 2013; 6(1): 69-76.
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36. Mehranjani MS, Taefi R. The protective role of vitamin E on the tes ticular tissue in rats exposed to sodium arsenite during the prenatal s tage till sex maturity: a s tereological analysis. Iran J Reprod Med. 2012; 10(6): 571-580.
36
37. Sönmez M, Türk G, Yüce A. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and tes tos terone levels of male Wis tar rats. Theriogenology. 2005; 63(7): 2063-2072.
37
38. Kammoun M, Miladi S, Ali YB, Damak M, Gargouri Y, Bezzine S. In vitro s tudy of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts. Lipids Health Dis. 2011; 10(1): 30.
38
ORIGINAL_ARTICLE
Assessment of Organophosphate Pesticides Exposure in Men with Idiopathic Abnormal Semen Analysis: A Cross-Sectional Pilot Study
Background: Because of the widespread use of organophosphate (OP) pesticides in agriculture, they are major environmental contaminants in developing countries. OP pesticides decrease sperm concentration and affect its quality, viability, and motility. Studies have demonstrated the association between abnormal semen analysis and OP pesticides exposure among the high-risk population. Asthere is limited data on the percentage of OP pesticides exposure, the study aimed to determine the OP pesticides exposure in Southern Indian men with idiopathic abnormal semen analysis and find the possible source of their OP pesticides exposure. Materials and Methods: In this cross-sectional pilot study, fifty men with idiopathic abnormal semen analysis as cases and fifty men with normal semen analysis as controls were recruited. Detailed history wastaken and general and systemic examinations were carried out. OP pesticides exposure was determined by assessment of pseudocholinesterase and acetylcholinesterase levels and urinary OP pesticides metabolites dialkyl phosphate (DAP) consisting of dimethyl phosphate (DMP), diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP). Results: Cases had statistically significantly lower levels of pseudocholinesterase (5792.07 ± 1969.89 vs. 10267.01 ± 3258.58 IU/L) (P=0.006) and acetylcholinesterase [102.90 (45.88-262.74) vs. 570.31 (200.24-975.30) IU/L] (P=0.001) as compared to controls. Cases had a statistically significantly higher percentage of urinary DAP positivity as compared to controls (80 vs. 38%) (p <0.0001). Hence, cases had a significantly higher percentage of OP pesticides exposure as compared to controls (20 vs. 4 %) (P=0.015). OP-exposed cases had significantly higher urinary DETP and DEDTP levels as compared to OP non-exposed cases. Also, urinary DETP and DEDTP levels were significantly negatively associated with sperm concentration, motility, and normal morphology among OP-exposed cases. Conclusion: Southern Indian men with idiopathic abnormal semen analysis had a significantly higher percentage of OP pesticides exposure as compared to men with a normal semen analysis.
https://www.ijfs.ir/article_47835_e9c29de65a47e4c696b8ca340e6251d8.pdf
2021-07-01
219
225
10.22074/ijfs.2020.134650
Acetylcholinesterase
Male infertility
Pseudocholinesterase
Organophosphate Pesticides
Induja
Manikandan
indu.5445@gmail.com
1
Department of Biochemis try, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India
AUTHOR
Sushmita
Bora
sushmitabora20@gmail.com
2
Department of Biochemis try, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India
AUTHOR
Prashant
Adole
prashant.adole@gmail.com
3
Department of Biochemis try, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India
LEAD_AUTHOR
Chitra
Thyagaraju
drchitra@yahoo.com
4
Department of Obs tetrics and Gynecology, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India
AUTHOR
Rajesh
Ganesh
drngrajesh@gmail.com
5
Department of Pathology, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India
AUTHOR
1. Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al. International Committee for Monitoring Assis ted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil S teril. 2009; 92: 1520-1524.
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8. Bonovoisin T, Utyasheva L, Knipe D, Gunnell D, Eddles ton M. Suicide by pes ticide poisoning in India: a review of pes ticide regulation and their impact on suicide trends. BMC Public Health. 2020; 20(1): 251.
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12. Contreras HR, Bus tos-Obregon E. Morphological alterations in mouse tes tis by a single dose of malathion. J Exp Zool. 1999; 284(3): 355-359.
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13. Ghafouri-Khosrowshahi A, Ranjbar A, Mousavi L, Nili-Ahmadabadi H, Ghaffari F, Zeinvand-Lores tani H, et al. Chronic exposure to organophosphate pes ticides as an important challenge in promoting reproductive health: a comparative s tudy. J Educ Health Promot. 2019; 8: 149.
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14. Miranda-Contreras L, Cruz I, Osuna JA, Gómez-Pérez R, Berrueta L, Salmen S, et al. Effects of occupational exposure to pes ticides on semen quality of workers in an agricultural community of Merida s tate, Venezuela. Inves t Clin. 2015; 56(2): 123-136.
14
15. Cremonese C, Piccoli C, Pasqualotto F, Clapauch R, Koifman RJ, Koifman S, et al. Occupational exposure to pes ticides, reproductive hormones levels and sperm quality in young Brazilian men. Reprod Toxicol. 2017; 67: 174-185.
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16. Yucra S, Gasco M, Rubio J, Gonzales GF. Semen quality in Peruvian pes ticide applicators: association between urinary organophosphate metabolites and semen parameters. Environ Health. 2008; 7: 59.
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17. Jamal F, Haque QS, Singh S, Ras togi SK. The influence of organophosphate and carbamate on sperm chromatin and reproductive hormones among pes ticide sprayers. Toxicol Ind Health. 2016; 32(8): 1527-1536.
17
18. Daoud S, Sellami A, Bouassida M, Kebaili S, Ammar Keskes L, Rebei T, et al. Routine assessment of occupational exposure and its relation to semen quality in infertile men: a cross-sectional s tudy. Turk J Med Sci. 2017; 47(3): 902-907.
18
19. Lotti F, Corona G, Vitale P, Maseroli E, Fino MG, Maggi M. Current smoking is associated with lower seminal vesicles and ejaculate volume, despite higher tes tos terone levels, in male subjects of infertile couples. Hum Reprod. 2015; 30(3): 590-602.
19
20. Matsumoto S, Beeson WL, Shavlik DJ, Siapco G, Jaceido-Sieg l, Fraser G, et al. Association between vegetarian diet and cardiovascular risk factors in non-Hispanic white participants of the Adventis t Health S tudy-2. J Nutr Sci. 2019; 8: e6.
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21. Dalvi TM, Khairnar MR, Kalghatgi SR. An Update of B.G. Prasad and Kuppuswamy socioeconomic s tatus classification scale for Indian population. Indian J Pediatr. 2020; 87(7): 567-568.
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22. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University Press; 1999.
22
23. Ellman GL, Courtney KD, Andres V Jr, Feather-S tone RM. A new and rapid colorimetric determination of acetylcholines terase activity. Biochem Pharmacol. 1961; 7: 88-95.
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24. Hemakanthi De Alwis GK, Needham LL, Barr DB. Determination of dialkyl phosphate metabolites of organophosphorous pes ticides in human urine by automated solid-phase extraction, derivatization and gas chromatography mass spectrometry. J Anal Toxicol. 2008; 32(9): 721-727.
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25. Hornung RW, Reed LD. Es timation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg. 1990; 5(1): 46-51.
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26. Nicolopoulou-S tamati P, Maipas S, Kotampasi C, S tamatis P, Hens L. Chemical pes ticides and human health: The urgent need for a new concept in agriculture. Front Public Health. 2016; 4: 148.
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27. Nishant N, Upadhyay R. Presence of pes ticide residue in vegetable crops: a review. Agricultural Review. 2016; 37(3): 173-185.
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28. Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M. Occupational exposure to pes ticides and consequences on male semen and fertility: a review. Toxicol Lett. 2014; 230(2): 146-156.
28
29. Kaur K, Kaur R. Occupational pes ticide exposure, impaired DNA repair, and diseases. Indian J Occup Environ Med. 2018; 22(2): 74-81.
29
30. Rehman S, Usman Z, Rehman S, AlDraihem M, Rehman N,Rehman I, et al. Endocrine disrupting chemicals and impact on male reproductive health. Transl Androl Urol. 2018; 7(3): 490-503.
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31. Gracia-Lor E, Cas tiglioni S, Bade R, Cas trignanò E, González-Mariño I, Hapeshi E, et al. Measuring biomarkers in was tewater as a new source of epidemiological information: current s tate and future perspectives. Environ Int. 2017; 99: 131-150.
31
32. Glorennec P, Serrano T, Fravallo M, Warembourg C, Monfort C, Cordier S, et al. Determinants of children’s exposure to pyrethroid insecticides in wes tern France. Environ Int. 2017; 104: 76-82.
32
33. Katole A, Saoji AV. Prevalence of primary infertility and its associated risk factors in urban population of central India: a community-based cross-sectional s tudy. Indian J Community Med. 2019; 44(4): 337-341.
33
34. Dutta S, Bahadur M. Effect of pes ticide exposure on the cholines terase activity of the occupationally exposed tea garden workers of northern part of Wes t Bengal, India. Biomarkers. 2019; 24(4): 317-324.
34
35. Li AJ, Kannan K. Urinary concentrations and profiles of organophosphate and pyrethroid pes ticide metabolites and phenoxyacid herbicides in populations in eight countries. Environ Int. 2018; 121(Pt 2): 1148-1154.
35
36. Muñoz-Quezada MT, Iglesias V, Lucero B, S teenland K, Barr DB, Levy K, et al. Predictors of exposure to organophosphate pes ticides in schoolchildren in the Province of Talca, Chile. Environ Int. 2012; 47: 28-36.
36
ORIGINAL_ARTICLE
Effect of Different High-Fat and Advanced Glycation End-Products Diets in Obesity and Diabetes-Prone C57BL/6 Mice on Sperm Function
Background: We aimed to compare the effects of using high-fat (HF) and advanced glycation end-products (AGEs) containing dietsto induce obesity and diabetes on sperm function in mice. Materials and Methods: In this experimental study, twenty-five 4-week old C57BL/6 mice were divided into 5 groups and were fed with control, 45% HF, 60% HF, 45% AGEs-HF, or 60% AGEs-HF diet. After 28 weeks, fast blood sugar, glucose intolerance, insulin concentration, homeostatic model assessments (HOMA) for insulin resistance (IR) and HOMA for beta cells (HOMA beta) from systematic blood were assessed. In addition, body weight, morphometric characteristics of testes, sperm parameters, DNA damage (AO), protamine deficiency (CMAA3), and sperm membrane (DCFH-DA) and intracellular (BODIPY) lipid peroxidation were measured. Results: Body mass and fasting blood sugar increased significantly in all experimental groups compared to the control group. Insulin concentration, glucose intolerance, HOMA IR, and HOMA beta were also increased significantly with higher levels of fat and AGEs in all four diets (p <0.05). The changes in the 60% HF-AGEs group, however, were more significant (p <0.001). Morphometric characteristics of the testis, sperm concentration, and sperm morphology in the diet groups did not significantly differ from the control group, while sperm motility and DNA damage in the 45%HF were significantly low. Although for protamine deficiency, both 60% HF-AGEs and 45% HF showed a significant increase compared to the control, the mean of sperm lipid in the 45% HF group and intracellular peroxidation in the 60% HF-AGEs group had the highest and the lowest increases, respectively. Conclusion: Our results, interestingly, showed that isthe negative effects of a diet containing AGEs on examined parameters are lessthan those in HF diets. One possible reason is detoxification through the activation of the protective glyoxalase pathway asthe result of the chronic AGEs increase in the body.
https://www.ijfs.ir/article_241733_87a89546f68095f5e6f33f87845cdef8.pdf
2021-07-01
226
233
10.22074/ijfs.2021.137231.1022
Advanced Glycosylation End products
Diabetes Mellitus
High-fat diet
Reactive Oxygen Species
Sperm parameters
Fahimeh
Akbarian
f.akbarian@hotmail.com
1
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Mohsen
Rahmani
rahmani.mhn@gmail.com
2
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Marziyeh
Tavalaee
tavalaee.m@royaninstitute.org
3
1. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Navid
Abedpoor
abedpoor.navid@yahoo.com
4
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Mozhdeh
Taki
mozhdeh201420@gmail.com
5
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Kamran
Ghaedi
kamranghaedi@yahoo.com
6
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
AUTHOR
Mohammad Hossein
Nasr-Esfahani
mh.nasr-esfahani@royaninstitute.org
7
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Ins titute for Biotechnology, ACECR, Isfahan, Iran
LEAD_AUTHOR
1. American Diabetes Association. Diagnosis and classification of diabetes mellitus diabetes care. 2012; 35 Suppl 1: S64-S71.
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2. World Health Organization. Laboratory manual for the examination and processing of human semen. Cambridge: Cambridge Univ Press; 2010.
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3. Bettiga A, Fiorio F, Di Marco F, Trevisani F, Romani A, Porrini E, et al. The modern wes tern diet rich in advanced glycation end-products (AGEs): an overview of its impact on obesity and early progression of renal pathology. Nutrients. 2019; 11(8): 1748.
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4. Nevin C, McNeil L, Ahmed N, Murgatroyd C, Brison D, Carroll M. Investigating the glycating effects of glucose, glyoxal and methylglyoxal on human sperm. Sci Rep. 2018; 8: 9002.
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5. Vlassara H, Palace M. Diabetes and advanced glycation endproducts. J Intern Med. 2002; 251(2): 87-101.
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6. Niwas Jangir R, Chand Jain G. Diabetes mellitus induced impairment of male reproductive functions: a review. Curr Diabetes Rev. 2014; 10(3): 147-157.
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7. Karimi J, Goodarzi M, Tavilani H, Khodadadi I, Amiri I. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Diabetes Res Clin Pract. 2011; 91(1): 61-66.
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8. Cardoso AR, Kakimoto PA, Kowaltowski AJ. Diet-sensitive sources of reactive oxygen species in liver mitochondria: role of very long chain acyl-CoA dehydrogenases. PLoS One. 2013; 8(10): e77088.
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9. Nadal-Casellas A, Amengual-Cladera E, Proenza AM, Lladó I, Gianotti M. Long-term high-fat-diet feeding impairs mitochondrial biogenesis in liver of male and female rats. Cell Physiol Biochem. 2010; 26(3): 291-302.
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10. Satapati S, Sunny NE, Kucejova B, Fu X, He TT, Méndez-Lucas A, et al. Elevated TCA cycle function in the pathology of diet-induced hepatic insulin resistance and fatty liver. J Lipid Resh. 2012; 53(6): 1080-1092.
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11. Sandu O, Song K, Cai W, Zheng F, Uribarri J, Vlassara H. Insulin resistance and type 2 diabetes in high-fat–fed mice are linked to high glycotoxin intake. Diabetes. 2005; 54(8): 2314-2319.
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12. Andrikopoulos S, Blair AR, Deluca N, Fam BC, Proietto J. Evaluating the glucose tolerance test in mice. Am J Physiol Endocrinol Metab. 2008; 295(6) :E1323- E1332.
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13. Wang CY, Liao JK. A mouse model of diet-induced obesity and insulin resistance. Methods Mol Biol. 2012; 821: 421-433.
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14. Sun C, Li X, Liu L, Conet M, Guan Y, Fan Y, et al. Effect of fasting time on measuring mouse blood glucose level. Int J Clin Exp med. 2016; 9(2): 4186-4189.
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15. Wallace TM, Levy JC, Matthews DR. Use and abuse of HOMA modeling. Diabetes Care. 2004; 27(6): 1487-1495.
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17. Afiyani AA, Deemeh MR, Tavalaee M, Razi M, Bahadorani M, Shokrollahi B, et al. Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction. Mol Reprod Dev. 2014; 81(8): 766-776.
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18. Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA. Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod. 2007; 13(4): 203-211.
18
19. Kiani-Esfahani A, Tavalaee M, Deemeh MR, Hamiditabar M, Nasr-Esfahani MH. DHR123: an alternative probe for assessment of ROS in human spermatozoa. Sys t Biol Reprod Med. 2012; 58(3): 168-174.
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20. de Assis AM, Rieger DK, Longoni A, Battu C, Raymundi S, da Rocha RF, et al. High fat and highly thermolyzed fat diets promote insulin resistance and increase DNA damage in rats. Exp Biol Med (Maywood). 2009; 234(11): 1296-1304.
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21. Karimi J, Goodarzi M, Tavilani H, Khodadadi I, Amiri I. Increased receptor for advanced glycation end products in spermatozoa of diabetic men and its association with sperm nuclear DNA fragmentation. Andrologia. 2012; 44 Suppl 1: 280-286.
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22. Mallidis C, Agbaje IM, Rogers DA, Glenn JV, Pringle R, Atkinson AB, et al. Advanced glycation end products accumulate in the reproductive tract of men with diabetes. Int J Androl. 2009; 32(4): 295-305.
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23. Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, et al. Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus. Human Reprod. 2007; 22(8): 2169-2177.
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24. Ferramosca A, Moscatelli N, Di Giacomo M, Zara V. Dietary fatty acids influence sperm quality and function. Andrology. 2017; 5(3): 423-430.
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25. Lu YC, Sudirman S, Mao CF, Kong ZL. Glycoprotein from Mytilus edulis extract inhibits lipid accumulation and improves male reproductive dysfunction in high-fat diet-induced obese rats. Biomed Pharmacother. 2019; 109: 369-376.
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26. Ferramosca A, Conte A, Moscatelli N, Zara V. A high-fat diet negatively affects rat sperm mitochondrial respiration. Andrology. 2016; 4(3): 520-525.
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27. Portela JM, Tavares RS, Mota PC, Ramalho-Santos J, Amaral S. High glucose concentrations per se do not adversely affect human sperm function in vitro. Reproduction. 2015; 150(1): 77-84.
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28. Chen MC, Lin JA, Lin HT, Chen SY, Yen GC. Potential effect of advanced glycation end products (AGEs) on spermatogenesis and sperm quality in rodents. Food Funct. 2019; 10(6): 3324-3333.
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29. Chen SJ, Aikawa C, Yoshida R, Matsui T. Methylglyoxal-derived hydroimidazolone residue of plasma protein can behave as a predictor of prediabetes in Spontaneously Diabetic Torii rats. Physiol Rep. 2015; 3(8): e12477.
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30. Antognelli C, Mancuso F, Frosini R, Arato I, Calvitti M, Calafiore R, et al. Testosterone and follicle stimulating hormone-dependent glyoxalase 1 up-regulation sus tains the viability of porcine sertoli cells through the control of hydroimidazolone- and argpyrimidine-mediated NF-κB pathway. Am J Pathol. 2018; 188(11): 2553-2563.
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31. Jain M, Nagar P, Sharma A, Batth R, Aggarwal S, Kumari S, et al. GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic s tress tolerance. Sci Rep. 2018; 8(1): 5451.
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33. Norouzirad R, González-Muniesa P, Ghasemi A. Hypoxia in obesity and diabetes: potential therapeutic effects of hyperoxia and nitrate. Oxid Med Cell Longev. 2017; 2017: 5350267.
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36. Aitken RJ. DNA damage in human spermatozoa; important contributor to mutagenesis in the offspring. Transl Androl Urol. 2017; 6 Suppl 4: S761- S764.
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37. Chen Y, Wu Y, Gan X, Liu K, Lv X, Shen H, et al. Iridoid glycoside from Cornus officinalis ameliorated diabetes mellitus-induced testicular damage in male rats: Involvement of suppression of the AGEs/RAGE/p38 MAPK signaling pathway. J Ethnopharmacol. 2016; 194: 850-860.
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38. Charalampidou S, Simitsopoulou Μ, Skoura L, Tziomalos K, Koulourida V, Goulis DG. Soluble receptor for advanced glycation end products in male infertility. Hippokratia. 2017; 21(1): 19-24.
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40
ORIGINAL_ARTICLE
Determinants of Birth Intervals Using PWP-Gap Time Model: Tehran Case Study
Background: Total fertility rate (TFR) in Iran decreased from the year 2000 and recently Iran has experienced fertility rates below replacement level. Birth interval is one of the most important determinants of fertility levels and plays a vital role in population growth rate. Due to the importance of this subject, the aim of this study was analyzing three birth intervals using three Survival Recurrent Event (SRE) models. Materials and Methods: In a 2017 cross-sectional fertility survey in Tehran, 610 married women, age 15-49 years, were selected by multi-stage stratified random sampling and interviewed using a structured questionnaire. The effects of selected covariates on first, second and third birth intervals were fitted to the data using the Prentice-Williams- Peterson-Gap Time (PWP-GT) SRE model in SAS 9.4. Results: Calendar-period had a significant effect on all three birth intervals (p <0.01). The Hazard Rate (HR) for a short birth interval for women in the most recent calendar-period (2007-2017) was lower than for the other calendarperiods. Women’s migration influenced second (P=0.044) and third birth intervals (P=0.031). The HR for both birth intervals in migrant women was 1.298 and 1.404 times shorter, respectively than non-migrant women. Women’s employment (P=0.008) and place of residence (p <0.05) also had significant effects on second birth interval; employed women and those living in developed, completely-developed and semi-developed areas, compared to unemployed women and those living in developing regions, had longer second birth intervals. Older age at marriage age increased the HR for a short third birth interval (p <0.01). Conclusion: The analysis of birth interval patterns using an appropriate statistical method provides important information for health policymakers. Based on the results of this study, younger women delayed their childbearing more than older women. Migrant women, unemployed women and women who live in developing regions gave birth to their second child sooner than non-migrant employed women, and women who lived in more developed regions. The implementation of policies which change the economic and social conditions of families could prevent increasing birth intervals and influence the fertility rate.
https://www.ijfs.ir/article_241639_6b08c6ef7573b5a9b7ef6e15df7ca597.pdf
2021-07-01
234
240
10.22074/ijfs.2021.134701
Birth Interval
Fertility
Survival analysis
Arezoo
Bagheri
abagheri_000@yahoo.com
1
National Institute for Population Research, Tehran, Iran
AUTHOR
Mahsa
Saadati
mahsa.saadati@gmail.com
2
National Institute for Population Research, Tehran, Iran
LEAD_AUTHOR
1. Khan JR, Bari W, Latif AH. Trend of determinants of birth interval dynamics in Bangladesh. BMC Public Health. 2016; 16(1): 934.
1
2. Hosseini-Chavoshi M, Abbasi-Shavazi MJ, McDonald P. Fertility, marriage, and family planning in Iran: Implications for future policy. Population Horizons. 2016; 13(1): 31-40.
2
3. Poorolajal J. Resistance economy and new population policy in Iran. J Res Health Sci. 2017; 17(1): e00367.
3
4. Sajid A, Mehmood T. Etonogestrel implant (implanon); frequency of adverse effects caused by implantation of singlerod etonogestrel implant (implanon) in females seeking birth spacing after delivery of previous pregnancy. Professional Med J. 2017; 24(5): 685-689.
4
5. Erfani A, Nojomi M, Hosseini H. Prolonged birth intervals in Hamedan, Iran: variations and determinants. J Biosoc Sci. 2017; 50(4): 457-471.
5
6. Fallahzadeh H, Farajpour Z, Emam Z. Duration and determinants of birth interval in Yazd, Iran: a population study. Iran J Reprod Med. 2013; 11(5): 379-384.
6
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