%0 Journal Article %T Combination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells %J International Journal of Fertility and Sterility %I Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR) %Z 2008-076X %A Absalan, Forouzan %A Movahedin, Mansoureh %A Mowla, Seyed Javad %D 2008 %\ 11/01/2008 %V 2 %N 3 %P 115-120 %! Combination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells %K Co-culture %K Colonization %K Spermatogonial %K Cryptorchidism %R 10.22074/ijfs.2008.45722 %X Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. Materials and methods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (α-MEM) supplemented with 10% fetal calf serum (FCS) for three weeks. The identity of the cells was confirmed through immunocytochemistry against Oct-4 and Vimentin. Results Best results were achieved from the co-culture system spermatogonia which continued to proliferate, and eventually, type A spermatogonia colonies were found. Most of the cells in these colonies were Oct-4 positive. Conclusion Bilateral cryptorchid surgery model is a good model for enrichment of spermatogonial stem cells (SSCs). These cells can be used for molecular characterization, genetic manipulation and restoration of male fertility. %U https://www.ijfs.ir/article_45722_dee1edd08a746120c38980070717a866.pdf