%0 Journal Article %T The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche %J International Journal of Fertility and Sterility %I Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR) %Z 2008-076X %A Borzouie, Zahra %A Hekmatimoghaddam, Seyedhossein %A Jebali, Ali %D 2020 %\ 07/01/2020 %V 14 %N 2 %P 150-153 %! The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche %K Azoospermia %K Human Serum Albumin %K Scaffold %K Spermatogenesis %K Testis %R 10.22074/ijfs.2020.6086 %X Azoospermia is one of the challenging disorders affecting couples who are afflicted with infertility. Human testisderived cells (hTCs) are suitable candidates for the initiation of in-vitro spermatogenesis for these types of patients. The current study aimed to assess the proliferation of hTCs through the cell culture on the three-dimensional (3D) porous scaffolds. Cells harvested from the testicular sperm extraction (TESE) samples of the azoospermic patients were cultured on the 3D porous scaffolds containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) for two weeks. The proliferation/viability of the cells was assessed using the MTT assay, along with H&E histological staining method. The MTT assay showed that hTCs could stay alive on this scaffold with 50 and 66.66% viability after 7 and 14 days, respectively. Such viability was not significantly different when compared with cells grown on monolayer flask culture (P > 0.05). Therefore, 3D HSA/TCP NPs scaffolds could be used for the reconstitu- tion of the artificial human somatic testicular niche for future applications in regenerative medicine for male infertility. %U https://www.ijfs.ir/article_45595_b681cf4cd34889d6b72143cc3e45e95b.pdf