@article { author = {Absalan, Forouzan and Movahedin, Mansoureh and Mowla, Seyed Javad}, title = {Combination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells}, journal = {International Journal of Fertility and Sterility}, volume = {2}, number = {3}, pages = {115-120}, year = {2008}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2008-076X}, eissn = {2008-0778}, doi = {10.22074/ijfs.2008.45722}, abstract = {Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. Materials and methods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (α-MEM) supplemented with 10% fetal calf serum (FCS) for three weeks. The identity of the cells was confirmed through immunocytochemistry against Oct-4 and Vimentin. Results Best results were achieved from the co-culture system spermatogonia which continued to proliferate, and eventually, type A spermatogonia colonies were found. Most of the cells in these colonies were Oct-4 positive. Conclusion Bilateral cryptorchid surgery model is a good model for enrichment of spermatogonial stem cells (SSCs). These cells can be used for molecular characterization, genetic manipulation and restoration of male fertility.}, keywords = {Co-culture,Colonization,Spermatogonial,Cryptorchidism}, url = {https://www.ijfs.ir/article_45722.html}, eprint = {https://www.ijfs.ir/article_45722_dee1edd08a746120c38980070717a866.pdf} }