The Pregnancy Outcome of Singletons in IVF/ ICSI Cycles: A Cross-Sectional Study
Mahnaz
Ashrafi
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Robabeh
Khalesi
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Tahereh
Madani
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Kiandokht
Kiani
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Fatemeh
Rastegar
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Fatemeh
Shabani
Epidemiology Department, Royan Institute
author
text
article
2007
eng
Background: The aim of this study was to compare prenatal outcome of intra cytoplasmic sperm injection (ICSI) pregnancies with pregnancies obtained through in vitro fertilization (IVF).Materials and Methods: Retrospectively 532 pregnancies of ART cycles were assessed during 1999-2000. The main outcome measured including abortions, ectopic pregnancies, prematurity, low birth weight, cesarean section, prenatal mortality and malformation compared between IVF and ICSI groups.Results: The mean age was similar in both IVF and ICSI groups, as well as treatment protocols, and the number of transferred embryos. There were significant differences in duration of infertility, and times of ART cycles between groups (p <0.05).The total spontaneous abortion rate (19.09% in IVF and 16.42% in ICSI) showed no significant difference between groups. In addition, we could not find significantly differences about the first and second trimester abortion between two groups. ICSI patients experienced similar ectopic pregnancy rate (1.2%) to IVF (0.9%).The rate of cesarean section was 42.5% in ART cycles. ICSI group showed a significant difference with IVF in cesarean section rate (46.9% VS. 35.8%) (p=0.043). The rate of low birth weights and preterm labor at birth demonstrated similarity between ICSI and IVF groups (5.1% versus 6.4% and 5.4% versus 5.5%, respectively). In ICSI, seven congenital anomalies were detected. The rate of congenital anomalies was 2.08% in ICSI and 0.9% in IVF (p>0.05). Prenatal mortality was also similar between groups. Conclusion: erinatal outcome after ICSI showed similarity in the number of pathologies in comparison with IVF.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
93
102
https://www.ijfs.ir/article_45651_7826a7e01b70cdb8f3536dd6144c2002.pdf
dx.doi.org/10.22074/ijfs.2007.45651
Novel Approach of Differential Staining to Detect Necrotic Cells in Preimplantation Embryos
Seyyed Morteza
Hosseini
Clinical and Experimental Embryology Department, Royan Institute
author
Mehdi
Hajian
Clinical and Experimental Embryology Department, Royan Institute
author
Vajihe
Asgari
Anatomy Department, Isfahan University of Medical Sciences
author
Mohsen
Forozanfar
Faculty of Basic Science, Islamic Azad University, Marvdasht Branch
author
Parvaneh
Abedi
Clinical and Experimental Embryology Department, Royan Institute
author
Mohammad Hossein
Nasr-Esfahani
Clinical and Experimental Embryology Department, Royan Institute
author
text
article
2007
eng
Background: This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst.Materials and Methods: Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were then freed from residual dyes by thoroughly washing in warm phosphate buffer saline free of calcium and magnesium (PBS-), fixed in 2.5% glutharaldehyde and washed again in PBS- . Stained embryos afterwards were mounted in a drop of glycerol over a microscopic slide. Prepared samples were examined under an epifluorescent microscope using the same excitation wavelength (330-385nm) and barrier filter (400nm) to distinguish necrosed vs. viable blastomers as being appeared in red and blue, respectively.Results: Obtained results showed that in cells with altered cell membrane such as late apoptotic or necrotic cells, PI and H33342 readily enter through the cytoplasmic barriers and so the chromatin materials are stained by both, but since PI quenches bisbenzimide fluorescence, necrotic blastomeres are seen in red to pinky red, while live cells are seen just as blue.Conclusion: Obtained results clearly indicated that this novel approach can be used as a simple, feasible and precise method for every embryology lab and with all the mammalian blastocysts produced either in vitro or in vivo. The basic assay can be completed in 60 min, and valuable and reliable information can be obtained about the quality of the embryos.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
103
106
https://www.ijfs.ir/article_45652_f551dc5119a235e9ff2651ef3179bb08.pdf
dx.doi.org/10.22074/ijfs.2007.45652
Evaluation of Varicocele Frequency in Adolescents in the City of Isfahan
Homayoun
Abbasi
Embryology and Andrology Department, Royan Institute, Isfahan Campus
author
Amir
Ghanbarian
Urology Department, Isfahan University of Medical Sciences
author
Saeid
Salimi Khoozani
Embryology and Andrology Department, Royan Institute, Isfahan Campus
author
Mohammad Hossein
Nasr-Esfahani
Embryology and Andrology Department, Royan Institute, Isfahan Campus
author
text
article
2007
eng
Background Although varicocele can be treated, it is considered as one of the most common causes of infertility. This study was performed in order to estimate prevalence of varicocele in adolescents aging 12-14 years in the city of Isfahan. Materials and methods During this descriptive analytic research, 800 secondary school students who were 12 to 14 years old were randomly chosen from all the five areas of the city. Physical examination results were considered absolutely private. Examinations could screen grades I, II, III, unilateral and bilateral varicocele. Finally, recorded data was analyzed. Results From 800 students examined, 133 individuals had varicocele. 74 students out of 133 individuals with varicocele, had grade I (55.6%) in the left side, 44 students had grade II (33.1%) in the left side, 4 students had grade III (3%) in the left side, and 11 students had bilateral varicocele (8.3%). Conclusion Considering the significant frequency of varicocele among adolescents more attention must be paid to early treatment of varicocele in order to restore the testicular function and therefore, future fertility. However late treatment may lead to testicular atrophy and irreversible consequences. Thus examination, diagnosis and treatment of possible varicocele in individuals at this range of age is highly recommended. In addition, the results of this study highlight the importance of parents' education about the consequences of varicocele on the future fertility of their children.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
107
112
https://www.ijfs.ir/article_45653_e4ddb9420979fdbefe4bb999faf8ab69.pdf
dx.doi.org/10.22074/ijfs.2007.45653
Anti-Thyroid Peroxidase and Risk of Recurrent Spontaneous Abortion
Mahnaz
Ashrafi
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Reza
Salman Yazdi
Andrology Department, Royan Institute
author
Tahereh
Madani
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Alireza
Bazrafshan
Faculty Medicine, Iran University of Medical Sciences
author
text
article
2007
eng
Background Approximately 2-4% of all women have recurrent spontaneous abortion (RSA); however, the cause is determined in only 50% of cases. Recent studies have shown an association between thyroid autoantibodies as a sign of thyroid autoimmunity and abortion. The aim of the present study was to determine whether circulating anti-thyroid peroxidase (anti-TPO) was associated with RSA. Materials and methods In this observational analytic study, Sera from 58 non-pregnant women with a history of RSA and also 58 healthy, fertile subjects with at least one live birth as control (Aging from 18 to 45 years) were tested for thyroid peroxidase antibodies by means of a standard Anti-TPO ELISA kit. We used data collection forms and SPSS software for data analysis. Results Of 116 women, 8 (13.8%) of the control subjects and 12 (20.7%) of the women with a history of RSA had positive results for anti-TPO. There was not any significant association between presence of anti-TPO and RSA. Conclusion We did not find any correlation between the presence of TPO antibodies and abortion in women with a history of RSA. On the basis of this study, testing for anti-TPO doesn’t seem to be useful in the evaluation of patients with a history of RSA.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
113
116
https://www.ijfs.ir/article_45654_5d341f1170754e466a81174764f6d3ab.pdf
dx.doi.org/10.22074/ijfs.2007.45654
0ocyte Related Factors and Chance of Implantation
Tahereh
Madani
Institute Royan, Center Research Medicine Reproductive, Department Infertility Female and E
author
Mahnaz
Ashrafi
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
Kiandokht
Kiani
Endocrinology and Female Infertility Department, Reproductive Medicine Research Center, Royan Institute
author
sorraya
khafri
Epidemiology Department, Royan Institute of Infertility
author
text
article
2007
eng
Background Endometriosis has been widely implicated as one of the causes of chronic pelvic pain, dysmenorrhea and infertility. The purpose of our study was to define the presumed correlation between visual and histologic diagnostic features of endometriosis based on the peritoneal findings identified in laparoscopy as a diagnostic trial with a standardized technique. So, all the specimens including complete excised lesions suggestive of endometriosis and systematic biopsies of normal appearing pelvic peritoneum were analyzed. This study was performed in Mahdieh hospital and IRHRC1 between 2004 and 2005. Materials and methods A diagnostic study of 30 patients (14 to 45 years) undergoing diagnostic laparoscopy for the evaluation of chronic pelvic pain, infertility, dysmenorrhea and dyspareunia was carried out (average age was 28.6±5.14). All areas suggestive of endometriosis were excised and examined pathologically. Peritoneal biopsy specimens were obtained from areas of normal appearing peritoneum to rule out microscopic endometriosis. The positive predictive value, sensitivity, negative predictive value and specificity were determined for identified endometriosis versus the histological findings. Results The mean prevalence of abnormalities visually consistent with endometriosis was 63% while 42% confirmed histologically. The positive predictive value was 42.1%, sensitivity 88.8%, negative predictive 90.9% and specificity 47.6% for visual versus histological diagnosis of endometriosis. Conclusion A diagnosis of endometriosis should be established only after histologic confirmation.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
117
122
https://www.ijfs.ir/article_45659_8a4746ddb70eca50d66ebb82767d8b9e.pdf
dx.doi.org/10.22074/ijfs.2007.45659
Evaluation of Diagnostic Visual Findings at Laparoscopy in Endometriosis
Saghar
Salehpour
Gynecology Department, Infertility and Reproductive Health Research Center (IRHRC), Shaheed Beheshti Medical University
author
Hanieh
Zhaam
Pathology Department, Shaheed Beheshti Medical University
author
Mahnaz
Hakimifard
Gynecology Department, Shaheed Beheshti Medical University
author
Leila
Khalili
Gynecology Department, Shaheed Beheshti Medical University
author
Yaedollah
Azar Gashb
Research Consultant, Shaheed Beheshti Medical University
author
text
article
2007
eng
Background Endometriosis has been widely implicated as one of the causes of chronic pelvic pain, dysmenorrhea and infertility. The purpose of our study was to define the presumed correlation between visual and histologic diagnostic features of endometriosis based on the peritoneal findings identified in laparoscopy as a diagnostic trial with a standardized technique. So, all the specimens including complete excised lesions suggestive of endometriosis and systematic biopsies of normal appearing pelvic peritoneum were analyzed. This study was performed in Mahdieh hospital and IRHRC1 .between 2004 and 2005. Materials and methods A diagnostic study of 30 patients (14 to 45 years) undergoing diagnostic laparoscopy for the evaluation of chronic pelvic pain, infertility, dysmenorrhea and dyspareunia .(was carried out (average age was 28.6±5.14 All areas suggestive of endometriosis were excised and examined pathologically. Peritoneal biopsy specimens were obtained from areas of normal appearing peritoneum to rule out .microscopic endometriosis The positive predictive value, sensitivity, negative predictive value and specificity were .determined for identified endometriosis versus the histological findings . Results The mean prevalence of abnormalities visually consistent with endometriosis was 63% while 42% confirmed histologically. The positive predictive value was 42.1%, sensitivity 88.8%, negative predictive 90.9% and specificity 47.6% for visual versus histological diagnosis .of endometriosis. Conclusion A diagnosis of endometriosis should be established only after histologic confirmation.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
123
126
https://www.ijfs.ir/article_45660_c0de41e4209f0e19b7ca297f18b3495e.pdf
dx.doi.org/10.22074/ijfs.2007.45660
Access to Fertility Treatments for Homosexual and Unmarried Persons, through Iranian Law and Islamic Perspective
Reza
Omani Samani
Ethics Department, Royan Institute, Royan Institute
author
Ahmad
Vosough Taghi Dizaj
Ethics Department, Royan Institute, Royan Institute
author
Mohammad Reza
Rezania Moalem
Ethics Department, Royan Institute, Royan Institute
author
Seyed Taha
Merghati
Ethics Department, Royan Institute, Royan Institute
author
Leila
Alizadeh
Ethics Department, Royan Institute, Royan Institute
author
text
article
2007
eng
Background A family traditionally consisted of a married man, his wife, and their children. The new reproductive technologies including use of the third party as egg, sperm and embryo donation and also surrogacy have changed the traditional meanings of lineage, ancestry and family. The increasing number of lesbians and gays in addition to single women requesting fertility treatment from IVF centers in the world has brought a debate: “Can fertility treatment be offered to single persons or homosexuals?” According to ASRM and ESHRE taskforces, there is no such limitation, but as legislations and law, also ethical and social considerations depending on the culture, believes and religion of societies must be considered as limitations in some contexts like Iran. Materials and methods In this study we review the Iran law and also some Islamic resources about offering the fertility treatment to single mothers or homosexuals. Results Homosexuality is not accepted in Islamic contexts and is considered as a crime. Children should have a family with both mother and father, unless they lose one of them or both. In the latter situation Islamic court will hand over the custody of the child to someone else like uncle or grandfather. Conclusion We concluded that according to Islam and Iran’s law, fertility treatment cannot be offered to single mothers and homosexuals mostly because of welfare of the child.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
127
130
https://www.ijfs.ir/article_45661_d400b7d7b1dbb7fc5e9c9335973513ec.pdf
dx.doi.org/10.22074/ijfs.2007.45661
In Vitro Oocyte Maturation in Polycystic Ovarian Syndrome Patients
Fatemeh
Ramazanzadeh
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
Mansur
Malek Aksa
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
Said
Amanpour
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
Maryam
Bagheri
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
Mina
Jafarabadi
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
Maryam
Nazm Bojnordi
Vali-e-asr Reproductive Health Research Center, Tehran University of Medical Science
author
text
article
2007
eng
Prevalence of Polycystic Ovarian Syndrome(PCOS) in Iran is more than 6%. Therefore we encounter with many PCOS patients. In Vitro Maturation (IVM) of oocytes as an attractive method in ART is considered. Studies show that changes in culture conditions should be administered to make IVM protocol more successful .For this purpose in this study we have set up the beneficial cultures for IVM procedure. Fourteen PCOS patients received FSH, 75 IU or 150 IU per day for 3 days initiating on day 3 of menstruation. Oocyte retrieval was performed transvaginally using an ultrasound-guided 17-gauge single lumen needle and filtered through a 70 micron gauge filter. Viable oocytes were put to maturation in TCM-199 supplemented with 10% Patient serum, recombinant FSH, pyruvate, penicillin, streptomycin sulphate and human chorionic gonadotropin..Oocytes were then inseminated by ICSI. The results indicated that 43.4% of oocytes matured to metaphase II. After 48 hours 47.5 % of M II oocytes fertilized by ICSI and cleaved to 2- and 4-cell stage. No pregnancy observed in PCOS patients. The oocytes maturation rate (43.4%) and embryo formation (47.5 %) from immature oocytes obtained in our IVM and ICSI culture system indicate that the present system may be nearly good, even though the number of patients were too small to draw significant conclusions.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
131
134
https://www.ijfs.ir/article_45662_c4f875e8a30ac63bb0d810cc36e05faf.pdf
dx.doi.org/10.22074/ijfs.2007.45662
Oocyte Enucleation Phase in Mouse Cloning
Abdolhossein
Shahverdi
Embryology Department ,Royan Institute
author
Rahman
Fakhery
Embryology Department ,Royan Institute
author
Azam
Dalman
Embryology Department ,Royan Institute
author
text
article
2007
eng
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
1
v.
3
no.
2007
135
135
https://www.ijfs.ir/article_45663_ac3e5f9caa5cc13e5f4aefb1afb46987.pdf
dx.doi.org/10.22074/ijfs.2007.45663