An Update on Ovarian Aging and Ovarian Reserve Tests
Ramazan
Amanvermez
Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey
author
Migraci
Tosun
Department of Obstetrics and Gynecology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey
author
text
article
2016
eng
Ovaries are the female organs that age more quickly than other tissues such as the uterus, the pituitary gland or pancreas. Different from males, an interesting question is why and how the females lose fertility so rapidly. During the aging process, both the number and quality of the oocytes in the ovaries decrease and reach to a point beyond that no more viable offspring may be produced and the associated cyclic endocrinological activities cease, entering the menopause in females at an average age of 50 years. Females who delayed childbearing with or without their willing until their 30 years or 40 years constitute the largest portion of the total infertility population. Ovarian reserve tests (ORTs) provide an indirect estimate of a female’s diminishing ovarian reserve or remaining follicular pool. This article briefly reviews recent progresses in relation to ovarian aging and ORTs.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
411
415
https://www.ijfs.ir/article_45331_392a8e8d2eeccc91e75a3896a8c02dc7.pdf
dx.doi.org/10.22074/ijfs.2016.4591
Oxidative Stress during Ovarian Torsion in Pediatric and Adolescent Patients: Changing The Perspective of The Disease
Antonio Simone
Laganà
Unit of Gynecology and Obstetrics, Department of Human Pathology in Adulthood and Childhood, G. Barresi,
University of Messina, Messina, Italy
author
Vincenza
Sofo
Department of Biomedical Sciences, Dentistry and Morphological and Functional Imaging, University of
Messina, Messina, Italy
author
Francesca Maria
Salmeri
Department of Biomedical Sciences, Dentistry and Morphological and Functional Imaging, University of
Messina, Messina, Italy
author
Vittorio Italo
Palmara
Unit of Gynecology and Obstetrics, Department of Human Pathology in Adulthood and Childhood, G. Barresi,
University of Messina, Messina, Italy
author
Onofrio
Triolo
Unit of Gynecology and Obstetrics, Department of Human Pathology in Adulthood and Childhood, G. Barresi,
University of Messina, Messina, Italy
author
Milan Milosav
Terzić
University of Belgrade, School of Medicine, Belgrade, Serbia;4Clinic for Gynecology and Obstetrics, Clinical Center of Serbia, Belgrade, Serbia
author
Tito Silvio
Patrelli
5University of Parma, Parma, Italy;6Vicenza General Hospital, Vicenza, Italy
author
Adolf
Lukanovic
7Department of Gynecology and Obstetrics, University Clinical Center, Ljubljana, Slovenia
author
Eda Vrtcnik
Bokal
7Department of Gynecology and Obstetrics, University Clinical Center, Ljubljana, Slovenia
author
Giuseppe
Santoro
8Department of Biomedical Sciences and Morpho-Functional Images, University of Messina, Messina, Italy
author
text
article
2016
eng
Among the different causes of gynecological acute pelvic pain, ovarian torsion represents a surgical emergency. It is a rare case in the pediatric/adolescent aged group that must be included in the differential diagnosis of any girl with abdominal pain or pelvic/abdominal mass. Current recommendations suggest that laparoscopic detorsion should be performed in order to preserve the integrity of the ovaries and fertility, although oophoropexy may be considered in case of severe necrosis. Nevertheless, maintaining the circulation of the ovary after detorsion deteriorates the tissue injury and leads to a pathologic process called ischaemia/reperfusion (I/R) injury, which is characterized by oxidative stress. During the detorsion process, an excess amount of molecular oxygen is supplied to the tissues, and reactive species of oxygen (ROS) such as superoxide radical (O2-), hydrogen peroxide (H2O2), hydroxyl radical (OH•), as well as reactive nitrogen species (RNS) are produced in excess. ROS, RNS and their toxic products cause DNA damage and lipid peroxidation in the cellular and mitochondrial membranes, leading to cell death. In spite of attention on this topic, currently there is no shared and clear evidence about the use of anti-inflammatory and antioxidant agents to prevent I/R damage after laparoscopic ovarian detorsion. Considering this element, future research should aim to develop shared protocols for the clinical use (route of application, dosage and time of application) of antioxidants after laparoscopic management of this condition.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
416
423
https://www.ijfs.ir/article_45332_ba6e28ed90a2d1692f8361eeb043b908.pdf
dx.doi.org/10.22074/ijfs.2016.4598
Epidemiology of Uterine Myomas: A Review
Radmila
Sparic
Clinic for Gynecology and Obstetrics, Clinical Center of Serbia, Belgrade, Serbia
author
Ljiljana
Mirkovic
Clinic for Gynecology and Obstetrics, Clinical Center of Serbia, Belgrade, Serbia;Faculty of Medicine, University of Belgrade, Belgrade, Serbia
author
Antonio
Malvasi
Department of Obstetrics and Gynecology, Santa Maria Hospital, Bari, Italy;4International Translational Medicine and Biomodelling Research Group Department of Applied Mathematics,
Moscow Institute of Physics and Technol
author
Andrea
Tinelli
4International Translational Medicine and Biomodelling Research Group Department of Applied Mathematics,
Moscow Institute of Physics and Technology (State University), Moscow Region, Russia;5Division of Experimental End
author
text
article
2016
eng
Myomas are the most common benign tumors of the genital organs in women of childbearing age, causing significant morbidity and impairing their quality of life. In our investigation, we have reviewed the epidemiological data related to the development of myomas in order to homogenize the current data. Therefore, a MEDLINE and PubMed search, for the years 1990-2013, was conducted using a combination of keywords, such as "myoma," "leiomyoma," "fibroids," "myomectomy," "lifestyle," "cigarette," "alcohol," "vitamins," "diet," and "hysterectomy". Randomized controlled studies were selected based upon the authors’ estimation. Peer-reviewed articles examining myomas were sorted by their relevance and included in this research. Additional articles were also identified from the references of the retrieved papers and included according to authors’ estimation. Many epidemiologic factors are linked to the development of myomas; however, many are not yet fully understood. These factors include age, race, heritage, reproductive factors, sex hormones, obesity, lifestyle (diet, caffeine and alcohol consumption, smoking, physical activity and stress), environmental and other influences, such as hypertension and infection. Some of the epidemiological data is conflicting. Thus, more research is needed to understand all the risk factors that contribute to myoma formation and how they exactly influence their onset and growth.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
424
435
https://www.ijfs.ir/article_45333_50e7262682eaa3d81b3384a33ab8a7db.pdf
dx.doi.org/10.22074/ijfs.2016.4599
A Preliminary Report of A Low-Dose Step-Up Regimen of Recombinant Human FSH for Young Women Undergoing Ovulation Induction with IUI
Hsin-Fen
Lu
Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan
author
Fu-Shiang
Peng
Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan
author
Shee-Uan
Chen
Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan
author
Bao-Chu
Chiu
Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan
author
Szu-Hsing
Yeh
Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan
author
Sheng-Mou
Hsiao
Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan
author
text
article
2016
eng
Background The aim of this study was to evaluate the efficacy and safety of a recombinant human follicle stimulating hormone (r-FSH) low-dose step-up regimen for controlled ovarian hyperstimulation in patients undergoing ovulation induction (OI) with intrauterine insemination (IUI). Materials and Methods The study was conducted in the Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, New Taipei, Taiwan. In this prospective, observational study, consecutive infertile women (20-35 years) with regular menstrual cycles and a normal baseline FSH level were prospectively enrolled between January 2010 and September 2010. A starting dose of 112.5 IU/day r-FSH was administered on day 3 and increased by 37.5 IU/day every 2 days until a follicle ≥11 mm in diameter was present. Recombinant human chorionic gonadotropin (r-hCG) was administered when a follicle ≥18 mm was noted. Monifollicular development was defined as only one follicle with a diameter ≥16 mm. Clinical pregnancy was defined as a pregnancy diagnosed by ultrasonographic visualization of one or more gestational sacs. Results A total of 29 women and 30 cycles were included. The mean daily dose of r-FSH to achieve a follicle of ≥11 mm in diameter was 131.3 ± 23.6 IU and the mean total dose was 1030.0 ± 383.2 IU. Approximately 41% of the cycles were monofollicular. Clinical pregnancy was observed in 9 (30.0%) cycles, and a fetal heart beat was observed in 7 (23.3%). There were no multiple pregnancies. Mild ovarian hyperstimulation syndrome, which was resolved with conservative management, was observed in 3 (10.0%) cycles. Conclusion This r-FSH low-dose step-up regimen seems to be a feasible and practical method for OI in younger infertile women undergoing IUI.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
436
441
https://www.ijfs.ir/article_45334_1e80c19dac2b0dd1a9e35e9350a6116e.pdf
dx.doi.org/10.22074/ijfs.2016.4600
Effect of In Vitro Maturation Technique and Alpha Lipoic Acid Supplementation on Oocyte Maturation Rate: Focus on Oxidative Status of Oocytes
Saeed
Zavareh
School of Biology, Damghan University, Damghan, Iran;Institute of Biological Sciences, Damghan University, Damghan, Iran
author
Isaac
Karimi
Laboratory of Molecular and Cellular Biology, Department of Basic Veterinary Sciences, School of Veterinary
Medicine, Razi University, Kermanshah, Iran
author
Mojdeh
Salehnia
4Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Ali
Rahnama
School of Biology, Damghan University, Damghan, Iran
author
text
article
2016
eng
Background Therapeutic potential of in vitro maturation (IVM) in infertility is growing with great promise. Although significant progress is obtained in recent years, existing IVM protocols are far from favorable results. The first aim of this study was to investigate whether two step IVM manner change reactive oxygen species (ROS) and total anti- oxidant capacity (TAC) levels. The second aim was to find the effect of alpha lipoic acid (ALA) supplementation on oocyte maturation rate and on ROS/TAC levels during IVM. Materials and Methods In this experimental study, mouse germinal vesicle (GV) oocytes divided into cumulus denuded oocytes (DOs) and cumulus oocyte complexes (COCs) groups. GVs were matured in vitro in the presence or absence of ALA only for 18 hours (control) or with pre-culture of forskolin plus cilostamide for an additional 18 hours. Matured oocytes obtained following 18 and 36 hours based on experimental design. In parallel, the ROS and TAC levels were measured at different time (0, 18 and 36 hours) by 2',7'-dichlorodihydrofluorescein (DCFH) probe and ferric reducing/antioxidant power (FRAP) assay, respectively. Results Maturation rate of COCs was significantly higher than DOs in control group (P < 0.05), while there was no significant difference between COCs and DOs when were pre-cultured with forskolin plus cilostamide. ROS and TAC levels was increased and decreased respectively in DOs after 18 hours while in COCs did not change at 18 hours and showed a significant increase and decrease respectively at 36 hours (P < 0.05). ROS and TAC levels in the presence of ALA were significantly decreased and increased respectively after 36 hours (P < 0.05) whereas, maturation rates of COCs and DOs were similar to their corresponding control groups. Conclusion ALA decreased ROS and increased TAC but could not affect maturation rate of both COCs and DOs in one or two step IVM manner.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
442
451
https://www.ijfs.ir/article_45335_e0da29cf29240f7ecc40a05b48c10ff3.pdf
dx.doi.org/10.22074/ijfs.2016.4601
Increased Length of Awareness of Assisted Reproductive Technologies Fosters Positive Attitudes and Acceptance among Women
Chelsea
Fortin
Department of Psychology, Miami University, Oxford, OH, USA;Department of Obstetrics and Gynecology, Cleveland Clinic, Cleveland, OH, USA
author
Susanne
Abele
Department of Psychology, Miami University, Oxford, OH, USA
author
text
article
2016
eng
Background The field of infertility medicine has witnessed a surge of scientific developments in recent years, but research on public attitudes towards infertility treatments has remained minimal. This study examined the social and demographic factors that affect women’s attitudes towards assisted reproductive technology (ART) in general, as well as their opinions of specific issues related to ART. Materials and Methods This cross-sectional study was conducted from March 2011 to April 2011 by means of an online survey administered to a sample of 287 women. Results Women with a longer length of awareness of ART had significantly greater attitudinal favorability towards ART. Political affiliation was also significantly related to general attitudes, as well as several specific aspects of ART issues. Conclusion The results of this study suggest that several factors influence attitudes that women hold in regards to ART. Identifying some of these factors serves as a crucial starting point for devising strategies to increase public acceptance of ART.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
452
464
https://www.ijfs.ir/article_45336_0b3c87f4bdaaf9655c53f047a56b57c8.pdf
dx.doi.org/10.22074/ijfs.2016.4603
Sexual Satisfaction and Sexual Reactivity in Infertile Women: The Contribution of The Dyadic Functioning and Clinical Variables
Anna
Czyżkowska
Faculty of Psychology, University of Finance and Management in Warsaw, Warsaw, Poland
author
Katarzyna
Awruk
Faculty of Psychology, University of Finance and Management in Warsaw, Warsaw, Poland
author
Konrad
Janowski
Faculty of Psychology, University of Finance and Management in Warsaw, Warsaw, Poland
author
text
article
2016
eng
Background Infertility is a factor which has been linked to higher prevalence of sexual dysfunctions in women; however, ambiguous results have been reported about the impact of infertility on women’s sexual satisfaction. The purpose of this study was to compare sexual and dyadic functioning in infertile and fertile women. Furthermore, the associations between sexual variables and clinical variables (depressive symptoms, period trying to conceive, and treatment period) were assessed in infertile women sample. Materials and Methods The cross-sectional study involved 50 women with the history of infertility and 50 fertile women recruited from the general population. The Sexual Satisfaction Scale (SSS), Mell-Krat Scale (women’s version), Family Assessment Measure (FAM-III), and Beck Depression Inventory (BDI) were administered to all participants. Results Infertile women reported lower sexual satisfaction and more maladaptive patterns of dyadic functioning in comparison to the control group. As many as 45 (90%) of infertile women, compared to 13 (26%) of the control group, reported the scores on the Mell-Krat Scale indicative of the presence of dysfunctions in sexual reactivity (P≤0.001). Infertile women reported significantly higher levels of depressive symptoms than the women from the control group (P≤0.001). Negative correlations were observed between sexual satisfaction and dyadic functioning in both groups (P≤0.05); however, the patterns of these associations were different in infertile and fertile women. For example, negative correlations were found between satisfaction with control and task accomplishment, role performance, affective involvement, and values and norms in infertile women. However, these relationships were not observed in the control group. No correlations were revealed between sexual reactivity and dyadic functioning in infertile women and the control group. Negative correlations were observed between satisfaction with control and relationship duration and treatment period as well as between sexual reactivity and period of trying to conceive. Multiple regression analyses also revealed different predictors of sexual satisfaction in both groups: affective involvement (P≤0.05) and relationship duration (P≤0.05) in infertile women, whereas communication (P≤0.05), affective expression (P≤0.05) and depressive symptoms (P≤0.05) in the control group. Conclusion Infertility is an important factor affecting sexual and dyadic functioning and is linked to higher depressive symptoms in infertile women.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
465
476
https://www.ijfs.ir/article_45337_01b0007248a73e2795f6c10f416684ea.pdf
dx.doi.org/10.22074/ijfs.2016.4604
Menstrual Pattern following Tubal Ligation: A Historical Cohort Study
Shahideh Jahanian
Sadatmahalleh
Department of Midwifery and Reproductive Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Saeideh
Ziaei
Department of Midwifery and Reproductive Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Anoshirvan
Kazemnejad
Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Eesa
Mohamadi
Department of Nursing, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
text
article
2016
eng
Background Tubal ligation (TL) is recommended for women who have completed their family planning. The existence of the menstrual disorders following this procedure has been the subject of debate for decades. This study was conducted to identify the relationship between tubal ligation and menstrual disorders. Materials and Methods A historical cohort study was carried out on 140 women undergoing tubal ligation (TL group) and on 140 women using condom as the main contraceptive method (Non-TL group). They aged between 20 and 40 years and were selected from a health care center in Rudsar, Guilan Province, Iran, during 2013-2014. The two groups were comparable in demographic characteristics, obstetrical features and menstrual bleeding pattern using a routine questionnaire. A validated pictorial blood loss assessment chart (PBLAC) was also used to measure the menstrual blood loss. Results Women with TL had more menstrual irregularity than those without TL (24.3 vs. 10%, P=0.002). Women with TL had more polymenorrhea (9.3 vs. 1.4%, P=0.006), hypermenorrhea (12.1 vs. 2.1%, P=0.002), menorrhagia (62.9 vs. 22.1%, P < 0.0001) and menometrorrhagia (15.7 vs. 3.6%, P=0.001) than those without TL. There is a significant difference in the PBLAC score between women with and without TL (P < 0.0001). According to logistic regression, age odds ratio [(OR=1.08, con- fidence interval (CI):1.07-1.17, P=0.03)], TL (OR=5.95, CI:3.45-10.26, P < 0.0001) and cesarean section (OR=2.72, CI:1.49-4.97, P=0.001) were significantly associated with menorrhagia. Conclusion We found significant differences in menstrual disorders between women with and without TL. Therefore, women should be informed by the health providers regarding the advantages and disadvantages of TL before the procedures.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
477
482
https://www.ijfs.ir/article_45338_8d313ae1020538445f267fb4ed508031.pdf
dx.doi.org/10.22074/ijfs.2016.4605
Performance of Circulating Placental Growth Factor as A Screening Marker for Diagnosis of Ovarian Endometriosis: A Pilot Study
Cinzia
Zucchini
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy
author
Paola
De Sanctis
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy
author
Chiara
Facchini
Pelvic Endoscopy and Minimally Invasive Gynaecologic Surgery, St. Orsola Malpighi University Hospital, Bologna, Italy
author
Nadine
Di Donato
Pelvic Endoscopy and Minimally Invasive Gynaecologic Surgery, St. Orsola Malpighi University Hospital, Bologna, Italy
author
Giulia
Montanari
Pelvic Endoscopy and Minimally Invasive Gynaecologic Surgery, St. Orsola Malpighi University Hospital, Bologna, Italy
author
Valentina
Bertoldo
Pelvic Endoscopy and Minimally Invasive Gynaecologic Surgery, St. Orsola Malpighi University Hospital, Bologna, Italy
author
Antonio
Farina
Department of Medicine and Surgery DIMEC, Division of Prenatal Medicine, St. Orsola Malpighi Hospital, University of
Bologna, Bologna, Italy
author
Alessandra
Curti
Department of Medicine and Surgery DIMEC, Division of Prenatal Medicine, St. Orsola Malpighi Hospital, University of
Bologna, Bologna, Italy
author
Renato
Seracchioli
Pelvic Endoscopy and Minimally Invasive Gynaecologic Surgery, St. Orsola Malpighi University Hospital, Bologna, Italy
author
text
article
2016
eng
Background The aim of this study is to compare the circulating placental growth factor (PlGF) concentration in women with and without endometrioma to verify the performance of this marker to diagnose the disease. Materials and Methods In this case-control study, thirteen women with histological diagnosis of ovarian endometriosis were compared with women without endometriosis disease. PlGF plasma levels of endometriotic patients and controls were investigated using a fluorescence immunoassay technique. Results PlGF showed a direct correlation with body mass index (BMI) only in the control group (P=0.013). After adjustment for BMI values, PlGF median value in endometriosis group (14.7 pg/mL) resulted higher than in control group (13.8 pg/ mL, P=0.004). Conclusion PlGF is a promising peripheral blood marker that can discriminate between patients with and without ovarian endometriosis.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
483
489
https://www.ijfs.ir/article_45339_be6dae844d04d1f0179e9f6a089cd92b.pdf
dx.doi.org/10.22074/ijfs.2016.4606
Metabolic Syndrome in Patients with Polycystic Ovary Syndrome in Iran
Ziba
Zahiri
Reproductive Health Research Center, Obstetrics and Gynecology Department, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Seyedeh Hajar
Sharami
Reproductive Health Research Center, Obstetrics and Gynecology Department, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Forozan
Milani
Reproductive Health Research Center, Obstetrics and Gynecology Department, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Fereshteh
Mohammadi
Guilan University of Medical Sciences, Rasht, Iran
author
Ehsan
Kazemnejad
Guilan University of Medical Sciences, Rasht, Iran
author
Hannan
Ebrahimi
Reproductive Health Research Center, Obstetrics and Gynecology Department, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Seyedeh Fatemeh
Dalil Heirati
Reproductive Health Research Center, Obstetrics and Gynecology Department, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
text
article
2016
eng
Background The prevalence of metabolic syndrome (MetS) in polycystic ovary syndrome (PCOS) has been studied in different populations, but their results were so controversial regarding Iranian women. These controversial data indicated the need for more investigation of MetS characteristics in PCOS patients in our population. So this study aimed to evaluate the clinical and laboratory characteristics and metabolic features of patients with PCOS in Rasht. Materials and Methods This prospective cross sectional study was conducted on 215 PCOS women who lived in Rasht, north of Iran, from March 2010 to July 2012. The participants were then divided into two groups of women with MetS (n=62) and women without MetS (n=153). The diagnosis of PCOS and MetS were based on the Rotterdam 2003 criteria and the Adult Treatment Panel III (ATP III) criteria, respectively. Demographic characteristics, fertility characteristics, family history and laboratory findings were assessed. Results The prevalence of MetS in women with PCOS was 28.8%. In PCOS women of both groups, the waist circumference (WC) exceeded 88cm in 72.6%, hypertension [systolic blood pressure (SBP) and/or diastolic blood pressure (DBP) ≥130/85mm Hg] was prevalent in 9.3%, fasting blood sugar (FBS) level was ≥110 mg/dl in 6%, triglycerides (Tg) level were ≥150 mg/dl in 47%, and high-density lipoprotein (HDL) level was
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
490
496
https://www.ijfs.ir/article_45340_19f0d53a59a0b0064c94a7a0086850c2.pdf
dx.doi.org/10.22074/ijfs.2015.4607
Comparison of Pregnancy Outcome between Ultrasound- Guided Tubal Recanalization and Office-Based Microhysteroscopic Ostial Dilatation in Patients with Proximal Blocked Tubes
Emaduldin Mostafa
Seyam
Department of Obstetrics and Gynecology, College of Medicine, El Minya University, Minya, Egypt
author
Momen Mohamed
Hassan
Department of Obstetrics and Gynecology, College of Medicine, El Minya University, Minya, Egypt
author
Mohamed
Tawfeek Mohamed Sayed Gad
Department of Obstetrics and Gynecology, College of Medicine, El Minya University, Minya, Egypt
author
Hazem Salah
Mahmoud
Department of Obstetrics and Gynecology, Al Fayoum General Hospital, Faiyum, Egypt
author
Mostafa Gamal
Ibrahim
Department of Obstetrics and Gynecology, Al Fayoum General Hospital, Faiyum, Egypt
author
text
article
2016
eng
Background The current research to the best of my knowledge is the first to compare the pregnancy outcome between ultrasound-guided tubal recanalization (UGTR) using a special fallopian tubal catheter, and office-based micrhysteroscopic ostial dilatation (MHOD) using the same tubal catherter in infertile women with previously diagnosed bilateral proximal tubal obstruction (PTO). Materials and Methods This prospective study reported the pregnancy outcomes for 200 women in private infertility care center in Arafa hospital in Fayoum and in El Minya University Hospital in the period between January 2010 and October 2013 treated as outpatients for their bilateral PTO after the routine hysterosalpingography (HSG). A Cook’s catheter, special fallopian tubal catheter, were used to recanalize the blocked tubes in 100 women (group A) under UGTR, and the same Cook’s tubal catheter was used through 2mm microhysteroscope to cannulate both ostia using MHOD in another 100 women (group B). Pregnancy outcome was determined after the procedures for a 12-month period follow-up. Results The number of the recanalization of PTO was not significantly different between two groups. As of the 200 blocked fallopian tubes in group A, 140 tubes (70%) were successfully recanalized by passing the ultrasound-guided special cannula, while 150 tubes (75%) were successfully recanalized in group B, using the same tubal catheter through a 2mm microhysteroscope. The cumulative pregnancy rate after the two procedures was not statistically different between two groups. It was 25.9% in group A, while it was 26.3% in group B, after a 12-month period follow-up. Conclusion UGTR is highly recommended as the first step to manage infertile women due to PTO, as it is easier procedure; however, there is possible to obtain nearly similar results after MHOD.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
497
505
https://www.ijfs.ir/article_45341_1692e94860e0aebc49c1238260ca7198.pdf
dx.doi.org/10.22074/ijfs.2015.4608
Correlation of Serum CA-125 and Progesterone Levels with Ultrasound Markers in The Prediction of Pregnancy Outcome in Threatened Miscarriage
Maged
Al Mohamady
Departement of Obstetrics and Gynecology, Cairo University, Giza, Egypt
author
Ghada Abdel
Fattah
Departement of Obstetrics and Gynecology, Cairo University, Giza, Egypt
author
Eman
Elkattan
Departement of Obstetrics and Gynecology, Cairo University, Giza, Egypt
author
Rasha
Bayoumy
Departement of Obstetrics and Gynecology, Cairo University, Giza, Egypt
author
Dalia Ahmed
Hamed
Department of Chemical Pathology, Cairo University, Giza, Egypt
author
text
article
2016
eng
Background The aim of this study was to evaluate the relationship between ultrasonographic findings and serum progesterone and cancer antigen-125 (CA-125) levels in threatened miscarriage and to predict pregnancy outcome. Materials and Methods In a prospective comparative case-control study, serum CA-125 and progesterone levels were measured for 100 pregnant women with threatened miscarriage who attended the outpatient clinic or the causality department of Obstetrics and Gynecology at Kasr El-Aini Hospital, Giza, Egypt, during the period from March 2013 to October 2013. Ultrasound was performed for fetal viability, crown-rump length (CRL), gestational sac diameter (GSD) and fetal heart rate (FHR). The patients were followed up and divided into two groups based on the outcome: 20 women who miscarried (group 1), and 80 women who continued pregnancy (group 2). The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall accuracy were tested for CA-125 and progesterone levels in prediction of the pregnancy outcome. Correlation of these chemical markers with the ultrasound markers was also examined. Results In the group that miscarried, CA-125 level was significantly higher (P < 0.001) and serum progesterone level was significantly lower (P < 0.001). For prediction of the outcome of pregnancy, the cut-off limit of 31.2 IU/ml for CA-125 level yielded sensitivity, specificity and an overall accuracy of 96.2, 100 and 99.4% respectively. The cut-off limit of 11.5 ng/ml for progesterone level yielded sensitivity, specificity and an overall accuracy of 97.5, 100 and 99.8% respectively. CA-125 level had a negative correlation with progesterone level and FHR levels (r=-0.716, P < 0.001) and (r=-0.414, P < 0.001) respectively. Serum progesterone level correlated with GSD (r=0.521, P < 0.001) and with CRL (r=0.407, P < 0.001) and FHR (r=0.363, P < 0.001). CA-125 level was significantly higher in the group that showed hematoma as compared with the group without hematoma (P < 0.001). Also, serum progesterone level was significantly lower in the group that showed hematoma as compared with the group without hematoma (P=0.017). Conclusion Serum CA-125 and progesterone levels are valid early predictors of the outcome of pregnancy in women with threatened miscarriage. They are correlated with some ultrasonographic markers (GSD, CRL, and FHR).
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
506
511
https://www.ijfs.ir/article_45342_a31acec5bf1a93e052c5fa3736bb5a41.pdf
dx.doi.org/10.22074/ijfs.2016.4609
Comparison of Toxicity of CdSe: ZnS Quantum Dots on Male Reproductive System in Different Stages of Development in Mice
Gholamreza
Amiri
Department of Physic, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
author
Akram
Valipoor
Department of Physiology, Basic Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
author
Kazem
Parivar
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Mehrdad
Modaresi
4Department of Physiology, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
author
Ali
Noori
5Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
author
Hamideh
Gharamaleki
6Department of Biology, Payame Noor University, Tehran, Iran
author
Jafar
Taheri
7Department of Chemistry, Islamic Azad University, Shahrekord, Iran
author
Ali
Kazemi
7Department of Chemistry, Islamic Azad University, Shahrekord, Iran
author
text
article
2016
eng
Background Quantum dots (QDs) are new types of fluorescent materials for biological labeling. QDs toxicity study is an essential requirement for future clinical applications. Therefore, this study aimed to evaluate cytotoxic effects of CdSe: ZnS QDs on male reproductive system. Materials and Methods In this experimental study, the different concentrations of CdSe: ZnS QDs (10, 20 and 40 mg/kg) were injected to 32 male mice (adult group) and 24 pregnant mice (embryo group) on day 8 of gestation. The histological changes of testis and epididymis were studied by a light microscopy, and the number of seminiferous tubules between two groups was compared. One-way analysis of variance (one-way Anova) using the Statistical Package for the Social Sciences (SPSS, SPSS Inc., USA) version 16 were performed for statistical analysis. Results In adult group, histological studies of testis tissues showed a high toxicity of CdSe: ZnS in 40 mg/kg dose followed by a decrease in lamina propria; destruction in interstitial tissue; deformation of seminiferous tubules; and a reduction in number of spermatogonia, spermatocytes, and spermatids. However, there was an interesting result in fetal testis development, meaning there was no significant effect on morphology and structure of the seminiferous tubules and number of sperm stem cells. Also histological study of epididymis tissues in both groups (adult and embryo groups) showed no significant effect on morphology and structure of tubule and epithelial cells, but there was a considerable reduction in number of spermatozoa in the lumen of the epididymal duct in 40 mg/kg dose of adult group. Conclusion The toxicity of QDs on testicular tissue of the mice embryo and adult are different before and after puberty. Due to lack of research in this field, this study can be an introduction to evaluate the toxicity of QDs on male reproduction system in different stages of development.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
512
520
https://www.ijfs.ir/article_45343_d204fb9fbec95ec9397b6ba471340f9d.pdf
dx.doi.org/10.22074/ijfs.2016.4610
Effects of Adding Sodium Nitroprusside to Semen Diluents on Motility, Viability and Lipid Peroxidation of Sperm in Holstein Bulls
Hamidreza
Khodaei
Depatment of Animal Science, Golpayegan Branch, Islamic Azad University, Golpayegan, Iran
author
Mohammad
Chamani
Department of Animal Physiology, Faculty of Agriculture and Natural Resources, Science and Research
Branch, Islamic Azad University, Tehran, Iran
author
Behnaz
Mahdavi
Depatment of Animal Science, Golpayegan Branch, Islamic Azad University, Golpayegan, Iran
author
Ali Asghar
Akhondi
Department of Animal Physiology, Faculty of Agriculture and Natural Resources, Science and Research
Branch, Islamic Azad University, Tehran, Iran
author
text
article
2016
eng
Background Nitric oxide (NO) that plays important role in all sexual activities of animals is made from the amino acid L-arginine by the enzymatic action of NO synthase (NOS). NO makes a band with sulfur-iron complexes, but due to production of steroid sexual hormones related to the enzymes involved in this complex, NO can change the activity of these enzymes. NO affects many cells including vein endothelial cells, macrophages and mast cells. These cells are also found in Leydig cells; therefore, they are important source of NO in testis tissue. Therefore, minimizing damages to sperm at the time of freezing thawing process are really important. The aim of this study was to determine the appropriate NO concentration to be added to the freezing extender to improve the quality of thawed sperm. Materials and Methods In this experimental randomized study, sperms of four Holstein bulls with an average age of 4 were collected twice a week for 3 weeks. They received sodium nitroprusside (SNP) in concentrations of 0, 10, 50 and 100 nmol/ml. Data analysis was performed using the special issue and static (SAS) 98 software. Also, mean comparison was done using Duncan’s multiple ranges test (P < 0.05).This research was conducted at the laboratory of Science and Research Branch, Islamic Azad University, Tehran at spring and summer of 2013. Results All concentrations of SNP used was found to increase motility and viability of spermatozoa at 1, 2 and 3 hours after thawing, significantly (P < 0.05), but there was no significant difference at zero time. Different concentrations of SNP reduced the membrane lipid peroxidation level of sperm and increased acrosome membranes integrity, implying that SNP generally improved samples membranes, especially in 50 and 100 nmol/ml concentrations. Conclusion According to the obtained results, addition of SNP to semen diluents increases motility and viability of spermatozoa. Also, it reduces membrane lipid peroxidation level that leads to improved sperm function.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
521
526
https://www.ijfs.ir/article_45344_d34725ee77745b779a780ae679dfbd0a.pdf
dx.doi.org/10.22074/ijfs.2016.4611
Accuracy Evaluation of The Depth of Six Kinds of Sperm Counting Chambers for both Manual and Computer-Aided Semen Analyses
Jin-Chun
Lu
Department of Laboratory Medicine, Nanjing Hospital, Jiangsu Corps, the Armed Police Force, PLA, Nanjing 210028,
Jiangsu, China
author
Ru-Qian
Yue
Geoffrey Laboratory for Semen Analysis, Jiangsu Jingcheng Pharmaceuticals Co., Ltd., Nanjing 210036, Jiangsu, China
author
Rui-Xiang
Feng
Department of Laboratory Medicine, Nanjing Hospital, Jiangsu Corps, the Armed Police Force, PLA, Nanjing 210028,
Jiangsu, China
author
Ling-Zhu
Kong
Geoffrey Laboratory for Semen Analysis, Jiangsu Jingcheng Pharmaceuticals Co., Ltd., Nanjing 210036, Jiangsu, China
author
Yuan-Cheng
Xu
Geoffrey Laboratory for Semen Analysis, Jiangsu Jingcheng Pharmaceuticals Co., Ltd., Nanjing 210036, Jiangsu, China
author
text
article
2016
eng
Background Although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. We measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy. Materials and Methods In this prospective study, the depths of six kinds of sperm counting chambers for both manual and computer-aided semen analyses, including Makler (n=24), Macro (n=32), Geoffrey (n=34), GoldCyto (n=20), Leja (n=20) and Cell-VU (n=20), were measured with the Filmetrics F20 Spectral Reflectance Thin-Film Measurement System, then the mean depth, the range and the coefficient of variation (CV) of each chamber, and the mean depth, relative deviation and acceptability of each kind of chamber were calculated by the closeness to the nominal value. Among the 24 Makler chambers, 5 were new and 19 were used, and the other five kinds were all new chambers. Results The depths (mean ± SD, μm) of Makler (new), Macro and Geoffrey chambers were 11.07 ± 0.41, 10.19 ± 0.48 and 10.00 ± 0.28, respectively, while those of GoldCyto, Leja and Cell-VU chambers were 23.76 ± 2.15, 20.49 ± 0.22 and 24.22 ± 2.58, respectively. The acceptability of Geoffrey chambers was the highest (94.12%), followed by Macro (65.63%), Leja (35%) and Makler (20%), while that of the other two kinds and the used Makler chamber was zero. Conclusion There existed some difference between the actual depth and the corresponding nominal value for sperm counting chambers, and the overall acceptability was very low. Moreover, the abrasion caused by the long use, as of Makler chamber, for example, may result in unacceptability of the chamber. In order to ensure the accuracy and repeatability of sperm concentration results, the depth of the sperm counting chamber must be checked regularly.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
527
533
https://www.ijfs.ir/article_45345_2a1cee00ae43f5db3f77730dc83564a1.pdf
dx.doi.org/10.22074/ijfs.2016.4612
Reducing Inter-Laboratory Differences between Semen Analyses Using Z Score and Regression Transformations
Esther
Leushuis
Department of Obstetrics and Gynecology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands;Academic Medical Center, Center for Reproductive Medicine, Amsterdam, The Netherlands
author
Alex
Wetzels
Radboud University Nijmegen Medical Centre, Department of Obstetrics and Gynaecology, Nijmegen,
The Netherlands
author
Jan Willem
van der Steeg
4Jeroen Bosch Ziekenhuis, Department of Obstetrics and Gynaecology, ’s-Hertogenbosch, The Netherlands
author
Pieternel
Steures
5University Medical Center Utrecht (UMC Utrecht), Department of Obstetrics and Gynaecology, Utrecht,
The Netherlands
author
Patrick M.M.
Bossuyt
6Academic Medical Center, Department of Clinical Epidemiology and Biostatistics, Amsterdam,
The Netherlands
author
Netty
van Trooyen
7Department of Clinical Chemical, St. Antonius Hospital, Nieuwegein, The Netherlands
author
Sjoerd
Repping
Radboud University Nijmegen Medical Centre, Department of Obstetrics and Gynaecology, Nijmegen,
The Netherlands
author
Frans A.L. van
der Horst
8Diagnostic Center SSDZ Reinier De Graaf Group, Department of Clinical Chemistry, Delft, The Netherlands
author
Peter G.A. Hompes
Hompes
Department of Obstetrics and Gynecology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands
author
Ben Willem J.
Mol
Academic Medical Center, Center for Reproductive Medicine, Amsterdam, The Netherlands
author
Fulco van
der Veen
Academic Medical Center, Center for Reproductive Medicine, Amsterdam, The Netherlands
author
text
article
2016
eng
Background Standardization of the semen analysis may improve reproducibility. We assessed variability between laboratories in semen analyses and evaluated whether a transformation using Z scores and regression statistics was able to reduce this variability. Materials and Methods We performed a retrospective cohort study. We calculated between-laboratory coefficients of variation (CVB) for sperm concentration and for morphology. Subsequently, we standardized the semen analysis results by calculating laboratory specific Z scores, and by using regression. We used analysis of variance for four semen parameters to assess systematic differences between laboratories before and after the transformations, both in the circulation samples and in the samples obtained in the prospective cohort study in the Netherlands between January 2002 and February 2004. Results The mean CVBwas 7% for sperm concentration (range 3 to 13%) and 32% for sperm morphology (range 18 to 51%). The differences between the laboratories were statistically significant for all semen parameters (all P < 0.001). Standardization using Z scores did not reduce the differences in semen analysis results between the laboratories (all P < 0.001). Conclusion There exists large between-laboratory variability for sperm morphology and small, but statistically significant, between-laboratory variation for sperm concentration. Standardization using Z scores does not eliminate between-laboratory variability.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
534
540
https://www.ijfs.ir/article_45346_05ae3d0f75e8a75f4f54f03312933913.pdf
dx.doi.org/10.22074/ijfs.2016.4613
Protective Effects of Thymoquinone against Methotrexate-Induced Germ Cell Apoptosis in Male Mice
Fatemeh
Sheikhbahaei
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Mozafar
Khazaei
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Arezou
Rabzia
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Kamran
Mansouri
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Ali
Ghanbari
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
text
article
2016
eng
Background Toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. This study investigated the protective effect of thymoquinone (TQ) as a fraction of Nigella sativa on methotrexate (MTX)- induced germ cell apoptosis in male mice. Materials and Methods In this experimental study, thirty male Balb/c mice were divided randomly into 5 groups (n=6). A single dose of MTX (20 mg/kg) and different concentrations of TQ were administrated for 4 consecutive days. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed on paraffin embedded tissue sections to analysis the occurrence of apoptosis in the testis. Reverse transcription polymerase chain reaction (RT-PCR) of apoptosis-related genes was performed with RNA extracted from testes of the mice. Statistical analysis was done using one-way ANOVA. Results In the MTX group, there was a significant increase in morphologic sign of germ cell degeneration of tubules (48 ± 0.6%), apoptotic index (AI; 2.3 ± 0.6%), as well as mRNA expression of p53 (P=0.008), caspase 8 (P=0.002), caspase 3 (P=0.005), caspase 9 (P=0.000), bax (P=0.004) and the ratio of bax/bcl-2 (P=0.000), whereas there was an decrease in the expression of bcl-2 (P=0.003), as compared to control group. In MTX+TQ groups, the data showed that different concentrations of TQ could improve the harmful effects caused by the MTX. The best protective effects were achieved in MTX+TQ (10 mg/kg). Conclusion TQ protects testicular germ cell against MTX-induced apoptosis by affecting related genes regulation.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
541
547
https://www.ijfs.ir/article_45347_1402297bf767a09879c6f360e456b36a.pdf
dx.doi.org/10.22074/ijfs.2016.4614
Protection against Cyclosporine-Induced Reprotoxicity by Satureja khuzestanica Essential Oil in Male Rats
Gholamreza
Najafi
Histology and Embryology Research Laboratories, Department of Basic Sciences, Faculty of Veterinary Medicine,
Urmia University, Urmia, Iran
author
Farah
Farokhi
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
author
Ali
Shalizar Jalali
Histology and Embryology Research Laboratories, Department of Basic Sciences, Faculty of Veterinary Medicine,
Urmia University, Urmia, Iran
author
Zahra
Akbarizadeh
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
author
text
article
2016
eng
Background The effects of cyclosporine (Cs), a fungal cyclic polypeptide with potent immunosuppressive activity, on fertility have assumed greater significance with the increasing numbers of transplantations being performed all over the world. Current study was undertaken to investigate the potential of Satureja khuzestanica Essential Oil (SEO) as an antioxidant to mitigate Cs-induced reprotoxicity. Materials and Methods In this experimental study (April-July 2012), thirty-two adult male Wistar rats were randomly divided into 4 groups of 8 animals each. Two groups of rats were administered Cs [40 mg/kg/day, per oral (p.o.)] for 45 days. One of these groups received SEO (225 mg/kg/day, p.o.) four hours after Cs administration. A vehicle-treated control group and a SEO control group were also included. Epididymal sperm characteristics, in vitro fertilizing capacity as well as embryo development were evaluated. For statistical analysis, one-way ANOVA and Tukey’s post-hoc test were used, and the value of P < 0.05 was considered as the criterion for statistical significance. Results Sperm count and viability along with fertilization and blastocyst development rates were significantly decreased by Cs treatment. Moreover, Cs-treated group showed significant increases in DNA damage, protamine deficiency of the sperm cells and proportion of spermatozoa with cytoplasmic droplet. Notably, aforementioned parameters were improved to near normal level by SEO co-administration. Conclusion These results suggest that SEO has a protective action against Cs-induced reprotoxicity in a rat model.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
548
557
https://www.ijfs.ir/article_45348_fdbf1b827f77ac4911725e459ebc1a2c.pdf
dx.doi.org/10.22074/ijfs.2016.4615
Prenatal Caffeine Exposure Impairs Pregnancy in Rats
Maryam
Yadegari
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Faculty of
Medicine, Yazd, Iran;Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kerman
author
Mozafar
Khazaei
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Morteza
Anvari
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Faculty of
Medicine, Yazd, Iran
author
Mohadeseh
Eskandari
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
text
article
2016
eng
Background In recent years, concerns have been raised about human reproductive disorders. Caffeine consumption is increasing by the world’s population and there is a relationship between caffeine intake and adverse reproductive outcomes. The aim of this study was to evaluate the effects of caffeine on implantation sites, number of live births, birth weight, crown-rump length (CRL) and abnormality in pregnant rats. Materials and Methods In this experimental study, 40 female albino rats (170-190 g) were randomly divided into two experimental and two control groups (n=10/each group). In both experimental groups, animals received caffeine intraperitoneally (IP: 150 mg/kg/day) on days 1-5 of pregnancy. In experimental group 1, treated animals were euthanized on day 7of pregnancy and the number of implantation sites was counted. In experimental group 2, treated animals maintained pregnant and after delivery, the number of live births, birth weight, CRL and abnormality of neonates were investigated. In control group, animals received IP injections of distilled water. Data were analyzed by independent t test. Results Results showed that administration of caffeine significantly decreased the number of implantation sites, number of live births and CRL as compared with control group (P < 0.05). There were no significant differences regarding birth weight and abnormality of neonate rats between experimental and control groups. Conclusion These results suggest that caffeine caused anti-fertility effect and significantly decreased CRL in neonate rats.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
558
562
https://www.ijfs.ir/article_45349_aec8682a526e5f2111613a2ac0e973cb.pdf
dx.doi.org/10.22074/ijfs.2016.4616
The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells
Masood
Bazrgar
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, Iran;Department of Developmental Biology, University of Science and Culture, Tehran, Iran
author
Hamid
Gourabi
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, Iran
author
Poopak
Eftekhari-Yazdi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive
Biomedicine, ACECR, Tehran, Iran
author
Hamed
Vazirinasab
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, Iran
author
Mostafa
Fakhri
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine,
ACECR, Tehran, Iran
author
Fatemeh
Hassani
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive
Biomedicine, ACECR, Tehran, Iran
author
Mohamad
Chehrazi
4Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
author
Mojtaba Rezazadeh
Valojerdi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive
Biomedicine, ACECR, Tehran, Iran
author
text
article
2016
eng
Background A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. Materials and Methods In this cohort study, we used fluorescence in situ hybridi- zation (FISH) to reanalyze surplus blastocysts undergoing preimplantation genetic diagnosis (PGD) on day 3 postfertilization. They were randomly studied on days 6 or 7 following fertilization. Results Of the 30 analyzed blastocysts, mosaicism was observed in 26(86.6%), while 2(6.7%) were diploid, and 2(6.7%) were triploid. Of those with mosaicism, 23(88.5%) were determined to be diploid-aneuploid and 3(11.5%) were aneuploid mosaic. The total frequency of embryos with more than 50% diploid cells was 33.3% that was lower on day 7 in comparison with the related value on day 6 (P < 0.05); however, there were no differences when the embryos were classified according to maternal age, blastocyst developmental stage, total cell number on day 3, and embryo quality. Conclusion Although mosaicism is frequently observed in blastocysts, the prolonged single culture of blastocysts does not seem to increase the rate of normal cells.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
563
573
https://www.ijfs.ir/article_45350_c0a259beb9c45d075c868ddae0204763.pdf
dx.doi.org/10.22074/ijfs.2016.4617
A Case of Bilateral Testicular Tumors Subsequently Diagnosed as Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
Yan-Kun
Sha
Department of Nephrology, First Affiliated Hospital of Liaoning Medical University, Jinzhou City 121000, Liaoning
Province, China
author
Yan-Wei
Sha
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Lu
Ding
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Wei-Wu
Liu
Department of Radiation, The Second Hospital of Jilin University, Changchun City 130041, Jilin Province, China
author
Yue-Qiang
Song
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Jin
Lin
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Xue-Mei
He
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Ping-Ping
Qiu
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Ling
Zhang
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
Ping
Li
Reproductive Medicine Center, Maternal and Child Health Hospital of Xiamen City 361005, Xiamen City, Fujian
Province, China
author
text
article
2016
eng
21-hydroxylase deficiency (21-OHD) caused congenital adrenal hyperplasia (CAH) is a group of autosomal recessive genetic disorders resulting from mutations in genes involved with cortisol (CO) synthesis in the adrenal glands. Testicular adrenal rest tumors (TARTs) are rarely the presenting symptoms of CAH. Here, we describe a case of simple virilizing CAH with TARTs, in a 15-year-old boy. The patient showed physical signs of precocious puberty. The levels of blood adrenocorticotropic hormone (ACTH), urinary 17-ketone steroids (17-KS), dehydroepiandrosterone sulfate (DHEA-S), and serum progesterone (PRGE) were elevated, whereas those of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and CO were reduced. Computed tomography (CT) of the adrenal glands and magnetic resonance imaging (MRI) of the testes showed a soft tissue density (more pronounced on the right side) and an irregularly swollen mass (more pronounced on the left side), respectively. Pathological examination of a specimen of the mass indicated polygonal/circular eosinophilic cytoplasm, cord-like arrangement of interstitial cells, and lipid pigment in the cytoplasm. Immunohistochemistry results precluded a diagnosis of Leydig cell tumors. DNA sequencing revealed a hackneyed homozygous mutation, I2g, on intron 2 of the CYP21A2 gene. The patient’s symptoms improved after a three-month of dexamethasone therapy. Recent radiographic data showed reduced hyperplastic adrenal nodules and testicular tumors. A diagnosis of TART should be considered and prioritized in CAH patients with testicular tumors. Replacement therapy using a sufficient amount of dexamethasone in this case helps combat TART.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
574
580
https://www.ijfs.ir/article_45351_2ae9cdfab26e2d6ab354a12ef1209b44.pdf
dx.doi.org/10.22074/ijfs.2016.4618
Molecular Dissection Using Array Comparative Genomic Hybridization and Clinical Evaluation of An Infertile Male Carrier of An Unbalanced Y;21 Translocation: A Case Report and Review of The Literature
Alfredo
Orrico
Molecular Medicine Unit, Azienda Ospedaliera Universitaria Senese, Siena, Italy;Medical Genetics, Misericordia Hospital, Grosseto, Italy
author
Giuseppina
Marseglia
Diagnostic Genetic Unit, Department of Laboratory, Careggi University Hospital, Firenze, Italy
author
Chiara
Pescucci
Diagnostic Genetic Unit, Department of Laboratory, Careggi University Hospital, Firenze, Italy
author
Ambra
Cortesi
Medical Genetics, Misericordia Hospital, Grosseto, Italy
author
Paola
Piomboni
4Department of Molecular and Developmental Medicine, University of Siena, Azienda Ospedaliera Universitaria
Senese, Siena, Italy
author
Andrea
Giansanti
5Genitourinary Unit, University of Siena, Azienda Ospedaliera Universitaria Senese, Siena, Italy
author
Francesca
Gerundino
Diagnostic Genetic Unit, Department of Laboratory, Careggi University Hospital, Firenze, Italy
author
Roberto
Ponchietti
5Genitourinary Unit, University of Siena, Azienda Ospedaliera Universitaria Senese, Siena, Italy
author
text
article
2016
eng
Chromosomal defects are relatively frequent in infertile men however, translocations between the Y chromosome and autosomes are rare and less than 40 cases of Y-autosome translocation have been reported. In particular, only three individuals has been described with a Y;21 translocation, up to now. We report on an additional case of an infertile man in whom a Y;21 translocation was associated with the deletion of a large part of the Y chromosome long arm. Applying various techniques, including conventional cytogenetic procedures, fluorescence in situ hybridisation (FISH) analysis and array comparative genomic hybridization (array-CGH) studies, we identified a derivative chromosome originating from a fragment of the short arm of the chromosome Y translocated on the short arm of the 21 chromosome. The Y chromosome structural rearrangement resulted in the intactness of the entire short arm, including the sex-determining region Y (SRY) and the short stature homeobox (SHOX) loci, although translocated on the 21 chromosome, and the loss of a large part of the long arm of the Y chromosome, including azoospermia factor-a (AZFa), AZFb, AZFc and Yq heterochromatin regions. This is the first case in which a (Yp;21p) translocation has been ascertained using an array-CGH approach, thus reporting details of such a rearrangement at higher resolution.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
4
no.
2016
581
585
https://www.ijfs.ir/article_45352_5ba067c88c1f2fb49bee877d66a2f201.pdf
dx.doi.org/10.22074/ijfs.2015.4619