Comparison of 25-hydroxyvitamin D and Calcium Levels between Polycystic Ovarian Syndrome and Normal Women
Ashraf
Moini
Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran;Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute
author
Nooshin
Shirzad
Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran;4Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Endocr
author
Marzieh
Ahmadzadeh
Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Reihaneh
Hosseini
Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Ladan
Hosseini
Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Shahideh Jahanian
Sadatmahalleh
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran;5Department of Reproductive Health and Midwifery, Faculty
author
text
article
2015
eng
Background Given the relationship of vitamin D deficiency with insulin resistance syndrome as the component of polycystic ovary syndrome (PCOS), the main aim of this study was to compare serum level of 25hydroxyvitamin D [25(OH)D] between PCOS patients and normal individuals. Materials and Methods A cross sectional study was conducted to compare 25(OH)D level between117 normal and 125 untreated PCOS cases at our clinic in Arash Hospital, Tehran, Iran, during 2011-2012. The obtained levels of 25(OH)D were classified as follows: lower than 25 nmol/ml as severe deficiency, between 25-49.9 nmol/ml as deficiency, 50-74.9 nmol/ml as insufficiency, and above 75 nmol/ml asnormal. In addition, endocrine and metabolic variables were evaluated. Results Among PCOS patients, our findings shows 3(2.4%) normal, 7(5.6%) with insufficiency, 33(26.4%) with deficiency and 82(65.6%) with severe deficiency, whereas in normal participants, 5(4.3%) normal, 4(3.4%) with insufficiency, 28(23.9%) with deficiency and 80(68.4%) with severe deficiency. Comparison of 25(OH)D level between two main groups showed no significant differences (p= 0.65). Also, the calcium and 25(OH)D levels had no significant differences in patients with overweight (p=0.22) and insulin resistance (p=0.64). But we also found a relationship between 25(OH)D level and metabolic syndrome (p=0.01). Furthermore, there was a correlation between 25(OH)D and body mass index (BMI) in control group (p=0.01), while the C-reactive protein (CRP) level was predominantly higher in PCOS group (p < 0.001). Conclusion Although the difference of 25(OH)D level between PCOS and healthy women is not significant, the high prevalence of 25(OH)D deficiency is a real alarm for public health care system and may influence our results.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
1
8
https://www.ijfs.ir/article_45279_ab1f26a624dbc76a0cb7e13d6f5f2f78.pdf
dx.doi.org/10.22074/ijfs.2015.4201
Comparison between Unilateral and Bilateral Ovarian Drilling in Clomiphene Citrate Resistance Polycystic Ovary Syndrome Patients: A Randomized Clinical Trial of Efficacy
Ziba
Zahiri Sorouri
Reproductive Health Research Center, Department of Obstetrics and Gynecology, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Seyede Hajar
Sharami
Reproductive Health Research Center, Department of Obstetrics and Gynecology, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Zinab
Tahersima
Reproductive Health Research Center, Department of Obstetrics and Gynecology, Alzahra Hospital, Guilan University of
Medical Sciences, Rasht, Iran
author
Fatemeh
Salamat
Research Vice Chancellorship, Guilan University of Medical Sciences, Rasht, Iran
author
text
article
2015
eng
Background Laparoscopic ovarian drilling (LOD) is an alternative method to induce ovulation in polycystic ovary syndrome (PCOS) patients with clomiphene citrate (CC) resistant instead of gonadotropins. This study aimed to compare the efficacy of unilateral LOD (ULOD) versus bilateral LOD (BLOD) in CC resistance PCOS patients in terms of ovulation and pregnancy rates. Materials and Methods In a prospective randomized clinical trial study, we included 100 PCOS patients with CC resistance attending to Al-Zahra Hospital in Rasht, Guilan Province, Iran, from June 2011 to July 2012. Patients were randomly divided into two ULOD and BLOD groups with equal numbers. The clinical and biochemical responses on ovulation and pregnancy rates were assessed over a 6-month follow-up period. Results Differences in baseline characteristics of patients between two groups prior to laparoscopy were not significant (p > 0.05). There were no significant differences between the two groups in terms of clinical and biochemical responses, spontaneous menstruation (66.1 vs. 71.1%), spontaneous ovulation rate (60 vs. 64.4%), and pregnancy rate (33.1 vs. 40%) (p > 0.05). Following drilling, there was a significant decrease in mean serum concentrations of luteinizing hormone (LH) (p=0.001) and testosterone (p=0.001) in both the groups. Mean decrease in serum LH (p=0.322) and testosterone concentrations (p=0.079) were not statistically significant between two groups. Mean serum level of follicle stimulating hormone (FSH) did not change significantly in two groups after LOD (p > 0.05). Conclusion Based on results of this study, ULOD seems to be equally efficacious as BLOD in terms of ovulation and pregnancy rates (Registration Number: IRCT138903291306N2).
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
9
16
https://www.ijfs.ir/article_45280_676166bc6e7035de90c14317ab9abfcd.pdf
dx.doi.org/10.22074/ijfs.2015.4202
A Randomized Clinical Trial on Comparing The Cycle Characteristics of Two Different Initiation Days of Letrozole Treatment in Clomiphene Citrate Resistant PCOS Patients in IUI Cycles
Nayereh
Ghomian
Ovulation Dysfunction Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ashraf
Khosravi
Ovulation Dysfunction Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Nezhat
Mousavifar
Ovulation Dysfunction Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2015
eng
Background There are still many questions about the ideal protocol for letrozole (LTZ) as the commonest aromatase inhibitor (AI) used in ovulation induction. The aim of this study is to compare the ultrasonographic and hormonal characteristics of two different initiation times of LTZ in clomiphene citrate (CC) failure patients and to study androgen dynamics during the cycle. Materials and Methods This randomized clinical trial was done from March to November 2010 at the Mashhad IVF Center, a university based IVF center. Seventy infertile polycystic ovarian syndrome (PCOS) patients who were refractory to at least 3 CC treatment cycles were randomly divided into two groups. Group A (n=35) receiving 5 mg LTZ on cycle days 3-7 (CD3), and group B (n=35) receiving the same amount on cycle days 5-9 (CD5). Hormonal profile and ultrasonographic scanning were done on cycle day 3 and three days after completion of LTZ treatment (cycle day 10 or 12). Afterward, 5,000-10,000 IU human chorionic gonadotropin (hCG) was injected if at least one follicle ≥18 mm was seen in ultrasonographic scanning. Intrauterine insemination (IUI) has been done 36-40 hours later. The cycle characteristics, the ovulation and pregnancy rate were compared between two groups. The statistical analysis was done using Fisher’s exact test, t test, logistic regression, and Mann-Whitney U test. Results There were no significant differences between two groups considering patient characteristics. The ovulation rate (48.6 vs. 32.4% in group A and B, respectively), the endometrial thickness, the number of mature follicles, and length of follicular phase were not significantly different between the two groups. Conclusion LTZ is an effective treatment in CC failure PCOS patients. There are no significant differences regarding ovulation and pregnancy rates between two different protocols of LTZ starting on days 3 and 5 of menstrual cycle (Registration Number: IRCT201307096467N3).
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
17
26
https://www.ijfs.ir/article_45281_323729696acdd01084083146f6af35e6.pdf
dx.doi.org/10.22074/ijfs.2015.4204
Age-Specific Serum Anti-Mullerian Hormone and Follicle Stimulating Hormone Concentrations in Infertile Iranian Women
Alireza
Raeissi
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of
Medical Sciences, Yazd, Iran
author
Alireza
Torki
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of
Medical Sciences, Yazd, Iran
author
Ali
Moradi
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of
Medical Sciences, Yazd, Iran
author
Seyed Mehdi
Mousavipoor
Department of Biochemistry, International Campus of Shahid Sadoughi University of Medical Sciences,
Yazd, Iran
author
Masoud Doosti
Pirani
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of
Medical Sciences, Yazd, Iran
author
text
article
2015
eng
Background Anti-Müllerian hormone (AMH) is secreted by the granulosa cells of growing follicles during the primary to large antral follicle stages. Abnormal levels of AMH and follicle stimulating hormone (FSH) may indicate a woman’s diminished ability or inability to conceive. Our aim is to investigate the changes in serum AMH and FSH concentrations at different age groups and its correlation with ovarian reserves in infertile women. Materials and Methods This cross-sectional study analyzed serum AMH and FSH levels from 197 infertile women and 176 healthy controls, whose mean ages were 19-47 years. Sample collection was performed by random sampling and analyzed with SPSS version 16 software. Results There were significantly lower mean serum AMH levels among infertile women compared to the control group. The mean AMH serum levels from different ages of infertile and control group (fertile women) decreased with increasing age. However, this reduction was greater in the infertile group. The mean FSH serum levels of infertile women were significantly higher than the control group. Mean serum FSH levels consistently increased with increasing age in infertile women; however mean luteinizing hormone (LH) levels were not consistent. Conclusion We have observed increased FSH levels and decreased AMH levels with increasing age in women from 19 to 47 years of age. Assessments of AMH and FSH levels in combination with female age can help in predicting ovarian reserve in infertile women.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
27
32
https://www.ijfs.ir/article_45282_351fe00ffe305b07ec67f30f6f957239.pdf
dx.doi.org/10.22074/ijfs.2015.4205
The Outcomes of Intracytoplasmic Sperm Injection and Laser Assisted Hatching in Women Undergoing In Vitro Fertilization Are Affected by The Cause of Infertility
Hsin-Fen
Lu
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Far
Eastern Memorial Hospital, New Taipei, Taiwan
author
Fu-Shiang
Peng
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Far
Eastern Memorial Hospital, New Taipei, Taiwan
author
Shee-Uan
Chen
Department of Obstetrics and Gynecology, National Taiwan University Hospital,
Taipei, Taiwan
author
Bao-Chu
Chiu
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Far
Eastern Memorial Hospital, New Taipei, Taiwan
author
Szu-Hsing
Yeh
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Far
Eastern Memorial Hospital, New Taipei, Taiwan
author
Sheng-Mou
Hsiao
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Far
Eastern Memorial Hospital, New Taipei, Taiwan
author
text
article
2015
eng
Background We sought to determine the association between factors that affected clini- cal pregnancy and live birth rates in patients who underwent in vitro fertilization (IVF) and received intracytoplasmic sperm injection (ICSI) and/or laser assisted hatching (LAH), or neither. Materials and Methods In this retrospective cohort study, the records of women who underwent IVF with or without ICSI and/or LAH at the Far Eastern Memorial Hospital, Taipei, Taiwan between January 2007 and December 2010 were reviewed. We divided patients into four groups: 1. those that did not receive ICSI or LAH, 2. those that received ICSI only, 3. those that received LAH only and 4. those that received both ICSI and LAH. Univariate and multivariate analyses were performed to determine factors associated with clinical pregnancy rate and live birth rate in each group. Results A total of 375 women were included in the analysis. Oocyte number (OR=1.07) affected the live birth rate in patients that did not receive either ICSI or LAH. Mater- nal age (OR=0.89) and embryo transfer (ET) number (OR=1.59) affected the rate in those that received ICSI only. Female infertility factors other than tubal affected the rate (OR=5.92) in patients that received both ICSI and LAH. No factors were found to affect the live birth rate in patients that received LAH only. Conclusion Oocyte number, maternal age and ET number and female infertility fac- tors other than tubal affected the live birth rate in patients that did not receive ICSI or LAH, those that received ICSI only, and those that received both ICSI and LAH, respectively. No factors affected the live birth rate in patients that received LAH only. These data might assist in advising patients on the appropriateness of ICSI and LAH after failed IVF.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
33
40
https://www.ijfs.ir/article_45283_00ca0c4a7a91a20ad0ca42dd619e31a0.pdf
dx.doi.org/10.22074/ijfs.2015.4206
Are Uterine and Ovarian Artery Doppler Velocimetry Values Good Pregnancy Predictors in Clomiphene Citrate Cycles?
Ali Irfan
Guzel
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
Selcuk
Erkılınc
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
Irfan
Ozer
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
Aytekin
Tokmak
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
Ayse
Kurt Sahin
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
Mustafa
Ugur
Division of Infertility and Gynecological Endocrinology, Department of Obstetrics and Gynecology, Dr. Zekai Tahir Burak Women’s Health Research and Education Hospital, Ankara, Turkey
author
text
article
2015
eng
Background We conducted this prospective study to evaluate the prognostic significance of uterine and ovarian artery Doppler velocimetry in clomiphene citrate (CC) cycles. Materials and Methods A total of 80 patients with unexplained infertility were given 100 mg/day of CC from day 3 to day 7 of their cycles in this current prospective study. On cycle day 3, before administration of CC, each patient underwent Doppler transvaginal ultrasonography. The Doppler velocimetries of the right and left uterine and ovarian arteries were recorded and analyzed in association with demographic and clinical parameters. Results TheThere were 6 out of 80 patients who became pregnant, the overall pregnancy rate in this population was 7.5% for the current study. The cases were divided into two groups according to whether they became pregnant or not. Demographic characteristics showed no statistically significant differences between these groups (p > 0.05). However, the duration of infertility did show statistically significant differences between the groups. Doppler velocimetry was not statistically significantly different between the two groups. Conclusion Doppler velocimetry of the uterine and ovarian arteries is not a factor in the prognosis for pregnancy in CC cycles.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
41
46
https://www.ijfs.ir/article_45284_d7167ac4604727b082d0266531e2d5b4.pdf
dx.doi.org/10.22074/ijfs.2015.4207
Chromosomal Analysis of Couples with Repeated Spontaneous Abortions in Northeastern Iran
Saeedeh
Ghazaey
Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran;Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Fatemeh
Keify
Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Farzaneh
Mirzaei
Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Masumeh
Maleki
Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Semiramis
Tootian
Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Mitra
Ahadian
Pardis Clinical and Genetics Laboratory, Mashhad, Iran
author
Mohammad Reza
Abbaszadegan
Pardis Clinical and Genetics Laboratory, Mashhad, Iran;Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of
Medical Sciences, Mashhad, Iran
author
text
article
2015
eng
Background Cytogenetic study of reproductive wastage is an important aspect in determining the genetic background of early embryogenesis. Approximately 15 to 20% of all pregnancies in humans are terminated as recurrent spontaneous abortions (RSAs). The aim of this study was to detect chromosome abnormalities in couples with RSAs and to compare our results with those reported previously. Materials and Methods In this retrospective study, the pattern of chromosomal aberrations was evaluated during a six-year period from 2005 to 2011. The population under study was 728 couples who attended genetic counseling services for their RSAs at Pardis Clinical and Genetics Laboratory, Mashhad, Iran. Results In this study, about 11.7% of couples were carriers of chromosomal aberrations. The majority of abnormalities were found in couples with history of abortion, without stillbirth or livebirth. Balanced reciprocal translocations, Robertsonian translocations, inversions and sex chromosome aneuploidy were seen in these cases. Balanced reciprocal translocations were the most frequent chromosomal anomalies (62.7%) detected in current study. Conclusion These findings suggest that chromosomal abnormalities can be one of the important causes of RSAs. In addition, cytogenetic study of families who experienced RSAs may prevent unnecessary treatment if RSA are caused by chromosomal abnormalities. The results of cytogenetic studies of RSA cases will provide a standard protocol for the genetic counselors in order to follow up and to help these families.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
47
54
https://www.ijfs.ir/article_45285_69e499da3163085f37141b9a94bce865.pdf
dx.doi.org/10.22074/ijfs.2015.4208
mRNA Expression of VEGF and Its Receptors in Fallopian Tubes of Women with Ectopic Pregnancies
Nafise
Zarezade
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran;Department of Biochemistry, Payame Noor University, Tehra
author
Samane
Saboori Darabi
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran;Department of Biochemistry, Payame Noor University, Tehra
author
Fariba
Ramezanali
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran
author
Elham
Amirchaghmaghi
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran
author
Gholamreza
Khalili
Department of Epidemiology and Reproductive Health at Reproductive Epidemiology Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran
author
Reza
Aflatoonian
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for
Reproductive Biomedicine, ACECR, Tehran, Iran
author
text
article
2015
eng
Background Establishment of viable pregnancy requires embryo implantation and placentation. Ectopic pregnancy (EP) is a pregnancy complication which occurs when an embryo implants outside of the uterine cavity, most often in a fallopian tube. On the other hand, an important aspect of successful implantation is angiogenesis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor responsible for vascular development that acts through its receptors, VEGF receptor 1 (VEGFR1) and VEGFR2. This study aims to investigate mRNA expression of VEGF and its receptors in fallopian tubes of women who have EP compared with fallopian tubes of pseudo-pregnant women. We hypothesize that expression of VEGF and its receptors in human fallopian tubes may change during EP. Materials and Methods This was a case-control study. The case group consisted of women who underwent salpingectomy because of EP. The control group consisted of women with normal fallopian tubes that underwent hysterectomy. Prior to tubal sampling, each control subject received an injection of human chorionic gonadotropin (hCG) to produce a state of pseudo-pregnancy. Fallopian tubes from both groups were procured. We investigated VEGF, VEGFR1 and VEGFR2 mRNA expressions in different sections of these tubes (infundibulum, ampulla and isthmus) by reverse transcription polymerase chain reaction (RT-PCR) and quantitative PCR (Q-PCR). Results RT-PCR showed expressions of these genes in all sections of the fallopian tubes in both groups. Q-PCR analysis revealed that expressions of VEGF, VEGFR1 and VEGFR2 were lower in all sections of the fallopian tubes from the case group compared to the controls. Only VEGFR2 had higher expression in the ampulla of the case group. Conclusion Decreased expressions of VEGF, VEGFR1 and VEGFR2 in the EP group may have a role in the pathogenesis of embryo implantation in fallopian tubes.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
55
64
https://www.ijfs.ir/article_45286_cdd8e55e3d91b67864fca74b03643ca2.pdf
dx.doi.org/10.22074/ijfs.2015.4209
The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
Ali
Risvanli
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey
author
Ahmet
Godekmerdan
Deparment of Microbiology, Faculty of Medicine, University of Yildirim Beyazit, Ankara, Turkey
author
text
article
2015
eng
Background In fertility studies, it has been shown that transforming growth factor β (TGFβ) and interlukin 10 (IL-10) play very important roles in implantation, maternal immune tolerance, placentation and fetal development, and the release beginning of release for fetal and postnatal death. The present study aims to determine the effects of the postmating administration of neutralizing antibodies against IL-10 and TGFβ, which significantly impact pregnancy in females and the conception rates in mice via assessments of blood serum and uterine fluid concentrations of IL-2, IL-4, IL-6, IL-10, IL-17, interferon γ (IFNγ), Tumor necrosis factor α (TNFα), and TGFβ. Materials and Methods In this experimental study, 21 BALB/c strain female mice were mated and randomly divided into three groups. The mice in the first group were selected as the control group. The second group of animals was injected with 0.5 mg of anti-IL-10 after mating, while those in the third group were intraperitoneally injected with 0.5 mg of anti-TGFβ. The animals in all groups were decapitated on the 13thday after mating and their blood samples were taken. The uteri were removed to determine pregnancy. The mice’s uterine irrigation fluids were also obtained. We used the multiplex immunoassay technique to determine the cytokine concentrations in uterine fluid and blood serum of the mice. Results We observed no intergroup difference with respect to conception rates. A comparison of the cytokine concentrations in the uterine fluids of pregnant mice revealed higher TGFβ concentrations (p < 0.01) in the second group injected with the anti-IL-10 antibody compared with the other groups. There was no difference detected in pregnant animals with regards to both uterine fluid and blood serum concentrations of the other cytokines. Conclusion Post-mating administration of anti-IL-10 and anti-TGFβ antibodies in mice may not have any effect on conception rates.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
65
70
https://www.ijfs.ir/article_45287_6f49459aad8fc4769c714804850c4c2c.pdf
dx.doi.org/10.22074/ijfs.2015.4210
Effect of Human Umbilical Cord Mesenchymal Stem Cells Transplantation on Nerve Fibers of A Rat Model of Endometriosis
Yan
Chen
Department of Obstetrics and Gynecology of the Third Affiliated Hospital of Zhengzhou University,
Zhengzhou, Henan 450052, China
author
Dong
Li
Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
author
Zhe
Zhang
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China
author
Natsuko
Takushige
4Department of Obstetrics and Gynaecology, University of Sydney, Sydney, NSW, 2006, Australia
author
Bei-Hua
Kong
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China
author
Guo-Yun
Wang
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China
author
text
article
2015
eng
Background Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Some researchers have identified nerve fibers in endometriotic lesions in women with endometriosis. Mesenchymal stem cells (MSCs) have attracted interest for their possible use for both cell and gene therapies because of their capacity for self-renewal and multipotentiality of differentiation. We investigated how human umbilical cord-MSCs (hUC-MSCs) could affect nerve fibers density in endometriosis. Materials and Methods In this experimental study, hUC-MSCs were isolated from fresh human umbilical cord, characterized by flow cytometry, and then transplanted into surgically induced endometriosis in a rat model. Ectopic endometrial implants were collected four weeks later. The specimens were sectioned and stained immunohistochemically with antibodies against neurofilament (NF), nerve growth factor (NGF), NGF receptor p75 (NGFRp75), tyrosine kinase receptor-A (Trk-A), calcitonin gene-related peptide (CGRP) and substance P (SP) to compare the presence of different types of nerve fibers between the treatment group with the transplantation of hUC-MSCs and the control group without the transplantation of hUC-MSCs. Results There were significantly less nerve fibers stained with specific markers we used in the treatment group than in the control group (p < 0.05). Conclusion MSC from human umbilical cord reduced nerve fiber density in the treatment group with the transplantation of hUC-MSCs.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
71
80
https://www.ijfs.ir/article_45288_c15e853a5875816592493c5670cbd7e7.pdf
dx.doi.org/10.22074/ijfs.2015.4211
Exploration of Infertile Couples’ Support Requirements: A Qualitative Study
Fatemeh
Jafarzadeh-Kenarsari
Nursing and Midwifery Student Research Center, Faculty of Nursing and Midwifery, Isfahan University of Medical
Sciences, Isfahan, Iran
author
Ataollah
Ghahiri
Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences,
Isfahan, Iran
author
Mojtaba
Habibi
Family Research Institute, Shahid-Beheshti University, Tehran, Iran
author
Ali
Zargham-Boroujeni
4Nursing and Midwifery Care Research Center, Faculty of Nursing and Midwifery, Isfahan University of Medical
Sciences, Isfahan, Iran
author
text
article
2015
eng
Background Due to high prevalence of infertility, increasing demand for infertility treatment, and provision of high quality of fertility care, it is necessary for healthcare professionals to explore infertile couples’ expectations and needs. Identification of these needs can be a prerequisite to plan the effective supportive interventions. The current study was, therefore, conducted in an attempt to explore and to understand infertile couples’ experiences and needs. Materials and Methods This is a qualitative study based on a content analysis ap- proach. The participants included 26 infertile couples (17 men and 26 women) and 7 members of medical personnel (3 gynecologists and 4 midwives) as the key informants. The infertile couples were selected from patients attending public and private infertility treatment centers and private offices of infertility specialists in Isfahan and Rasht, Iran, during 2012-2013. They were selected through purposive sampling method with maximum variation. In-depth unstructured interviews and field notes were used for data gathering among infertile couples. The data from medical personnel was collected through semi-structured interviews. The interview data were analyzed using conventional content analysis method. Results Data analysis revealed four main categories of infertile couples’ needs, including: i. Infertility and social support, ii. Infertility and financial support, iii. Infertility and spiritual support and iv. Infertility and informational support. The main theme of all these categories was assistance and support. Conclusion The study showed that in addition to treatment and medical needs, infertile couples encounter various challenges in different emotional, psychosocial, communicative, cognitive, spiritual, and economic aspects that can affect various areas of their life and lead to new concerns, problems, and demands. Thus, addressing infertile couples’ needs and expectations alongside their medical treatments as well as provision of psychosocial services by development of patient-centered approaches and couple-based interventions can improve their quality of life and treatment results and also relieve their negative psychosocial consequences.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
81
92
https://www.ijfs.ir/article_45289_69e712b103022b97a31ec868d50740f1.pdf
dx.doi.org/10.22074/ijfs.2015.4212
Effect of Phosalone on Testicular Tissue and In Vitro Fertilizing Potential
Amir
Amniattalab
Department of Pathology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
author
Mazdak
Razi
Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University,
Urmia, Iran
author
text
article
2015
eng
Background The current study aimed to evaluate the effects of phosalone (PLN) as an organophosphate (OP) compound on testicular tissue, hormonal alterations and embryo development in rats. Materials and Methods In this experimental study, we divided 18 mature Wistar rats into three groups-control, control-sham and test (n=6 per group). Animals in the test group received one-fourth the lethal dose (LD50) of PLN (150 mg/kg), orally, once per day for 45 days. DNA laddering and epi-fluorescent analyses were performed to evaluate testicular DNA fragmentation and RNA damage, respectively. Serum levels of testosterone and inhibin-B (IN-B) were evaluated. Testicular levels of total antioxidant capacity (TAC), total thiol molecules (TTM) and glutathione peroxidase (GSH-px) were analyzed. Finally, we estimated sperm parameters and effect of PLN on embryo development. Two-way ANOVA was used for statistical analyses. Results There was severe DNA fragmentation and RNA damage in testicular tissue of animals that received PLN. PLN remarkably (p < 0.05) decreased testicular TAC, TTM and GSH-px levels. Animals that received PLN exhibited significantly (p < 0.05) decreased serum levels of testosterone and IN-B. Reduced sperm count, viability, motility, chromatin condensation and elevated sperm DNA damage were observed in the test group rats. PLN resulted in significant (p < 0.05) reduction of in vitro fertilizing (IVF) potential and elevated embryonic degeneration. Conclusion PLN reduced fertilization potential and embryo development were attributed to a cascade of impacts on the testicles and sperm. PLN promoted its impact by elevating DNA and RNA damages via down-regulation of testicular endocrine activity and antioxidant status.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
93
106
https://www.ijfs.ir/article_45290_87a2e6322f846f01a0108bf72829726e.pdf
dx.doi.org/10.22074/ijfs.2015.4213
Predictive Factors of Successful Microdissection Testicular Sperm Extraction in Patients with Presumed Sertoli Cell-Only Syndrome
Tahereh
Modarresi
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Hani
Hosseinifar
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Ali
Daliri Hampa
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Mohammad
Chehrazi
Department of Epidemiology and Reproductive Health at Reproductive Epidemiology Research Center,
Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
author
Jalil
Hosseini
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Faramarz
Farrahi
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Farid
Dadkhah
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Marjan
Sabbaghian
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran
author
Mohammad Ali
Sadighi Gilani
Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedi-
cine, ACECR, Tehran, Iran;Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Te
author
text
article
2015
eng
Background To evaluate predictive factors of successful microdissection-testicular sperm extraction (MD-TESE) in patients with presumed Sertoli cell-only syndrome (SCOS). Materials and Methods In this retrospective analysis, 874 men with non-obstructive azoospermia (NOA), among whom 148 individuals with diagnosis of SCOS in prior biopsy, underwent MD-TESE at Department of Andrology, Royan Institute, Tehran, Iran. The predictive values of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) levels, testicular volume, as well as male age for retrieving testicular sperm by MD-TESE were analyzed by multiple logistic regression analysis. Results Testicular sperm were successfully retrieved in 23.6% men with presumed SCOS. Using receiver operating characteristic (ROC) curve analysis, it was shown that sperm retrieval rate in the group of men with FSH values >15.25% was 28.9%. This was higher than the group of men with FSH ≤15.25 (11.8%). Conclusion Sperm retrieval rate (SRR) was 23.6% in men with presumed SCOS and FSH level can be a fair predictor for SPR at MD-TESE. MD-TESE appears to be recommendable in such cases (SCOS with high FSH concentration) with reasonable results.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
107
112
https://www.ijfs.ir/article_45291_9b093ce7aba2317d0c524d7298ab29e3.pdf
dx.doi.org/10.22074/ijfs.2015.4214
Study on The Effect of Royal Jelly on Reproductive Parameters in Streptozotocin-Induced Diabetic Rats
Elham
Ghanbari
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
author
Vahid
Nejati
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
author
Gholamreza
Najafi
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mozafar
Khazaei
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
author
Mohammad
Babaei
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2015
eng
Background Diabetes mellitus has a variety of structural and functional effects on the male reproductive system. Diabetes results in reduced sperm parameters and libido. The present study aims to investigate the effects of royal jelly (RJ) on reproductive parameters of testosterone and malondialdehyde (MDA) production in diabetic rats. Materials and Methods This experimental study was conducted on adult male Wistar rats. The animals were divided into four groups (n=8 per group): control, RJ, diabetic and diabetic treated with RJ. Diabetes was induced by intraperitoneal injection of 60 mg/kg body weight (BW) of streptozotocin (STZ). RJ, at a dose of 100 mg/kg BW was given by gavage. The duration of treatment was six weeks. After the treatment period the rats were sacrificed. The testes were weighed and changes in sperm count, motility, viability, deformity, DNA integrity and chromatin quality were analyzed. Serum testosterone and MDA concentrations of testicular tissue were determined. Data were analyzed by oneway ANOVA with p < 0.05 as the significant level. Results STZ-induced diabetes decreased numerous reproductive parameters in rats. Testicular weight, sperm count, motility, viability and serum testosterone levels increased in the diabetic group treated with RJ. There was a significant decrease observed in sperm deformity, DNA integrity, chromatin quality, and tissue MDA levels in diabetic rats treated with RJ compared to the diabetic group (p < 0.05). Conclusion RJ improved reproductive parameters such as testicular weight, sperm count, viability, motility, deformity, DNA integrity, chromatin quality, serum testosterone and testicular tissue MDA levels in diabetic rats.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
113
120
https://www.ijfs.ir/article_45292_489e62fd4b5c5af6df562af47e88a8fa.pdf
dx.doi.org/10.22074/ijfs.2015.4215
Oxidant and Antioxidant Status in Experimental Rat Testis after Testicular Torsion/Detorsion
Tatjana
Cvetkovic
Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia;Clinic of Nephrology, Clinical Centre Nis, Nis, Serbia
author
Jablan
Stankovic
Clinic of Urology, Clinical Centre Nis, Nis, Serbia
author
Stevo
Najman
4Department of Biology, School of Medicine, University of Nis, Nis, Serbia
author
Dusica
Pavlovic
Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia
author
Dragana
Stokanovic
5Department of Pharmacology and Toxicology, School of Medicine, University of Nis, Nis, Serbia
author
Slobodan
Vlajkovic
6Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia
author
Marija
Dakovic-Bjelakovic
6Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia
author
Jovana
Cukuranovic
6Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia
author
Vladimir
Zivkovic
6Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia
author
Vladisav
Stefanovic
7School of Medicine, University of Nis, Nis, Serbia
author
text
article
2015
eng
Background The aim of this study was to determine oxidative stress (OS) parameters after testicular torsion/detorsion in adult rats. Materials and Methods In this experimental study, male adult Wistar rats were divided into four groups, each consisting of seven animals: group I-one hour right testicular torsion with subsequent orchiectomy, group II-one hour right testicular torsion followed by detorsion, group III-unilateral right-sided orchiectomy without previous torsion and group IV-control. After 30 days, bilateral orchiectomies were performed in rats with both testes and unilateral orchiectomies in rats with single testicles. Parameters of OS were determined in testicular tissue and in plasma. Results Plasma concentrations of advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS) were higher (p < 0.05 and p < 0.01, respectively), whilst the plasma concentration of the total sulfhydryl (T-SH)-groups was lower (p < 0.05) in group I compared to the control group. Group II had higher plasma concentrations of AOPP compared to group IV (p < 0.05), as well as significantly increased TBARS and decreased T-SH-group levels compared to groups III (p < 0.05 and p < 0.01, respectively) and IV (p < 0.01, for both parameters). There were significant differences in OS markers between the ipsilateral and contralateral testis, as well as significant correlations among levels of both plasma and tissue markers of OS. Conclusion The increase in TBARS levels seen throughout the experimental period indicated that OS development was caused by ischemia/reperfusion in the testicular tissue. The oxidant-antioxidant system of the testicular tissue was altered during torsion as well as detorsion.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
121
128
https://www.ijfs.ir/article_45293_8078ad4e6cb6fcaea8ff04ad7a796c6f.pdf
dx.doi.org/10.22074/ijfs.2015.4216
In Vitro Effect of Cell Phone Radiation on Motility, DNA Fragmentation and Clusterin Gene Expression in Human Sperm
Adel
Zalata
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt
author
Ayman Z
El-Samanoudy
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt
author
Dalia
Shaalan
Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt
author
Youssef
El-Baiomy
Department of Dermatology and Andrology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
author
Taymour
Mostafa
Department of Andrology and Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt
author
text
article
2015
eng
Background Use of cellular phones emitting radiofrequency electromagnetic field (RF-EMF) has been increased exponentially and become a part of everyday life. This study aimed to investigate the effects of in vitro RF-EMF exposure emitted from cellular phones on sperm motility index, sperm DNA fragmentation and seminal clusterin (CLU) gene expression. Materials and Methods In this prospective study, a total of 124 semen samples were grouped into the following main categories: i. normozoospermia (N, n=26), ii. asthenozoospermia (A, n=32), iii. asthenoteratozoospermia (AT, n=31) and iv. oligoasthenoteratozoospermia (OAT, n=35). The same semen samples were then divided into two portions non-exposed and exposed samples to cell phone radiation for 1 hour. Before and immediately after exposure, both aliquots were subjected to different assessments for sperm motility, acrosin activity, sperm DNA fragmentation and CLU gene expression. Statistical differences were analyzed using paired t student test for comparisons between two sub-groups where p < 0.05 was set as significant. Results There was a significant decrease in sperm motility, sperm linear velocity, sperm linearity index, and sperm acrosin activity, whereas there was a significant increase in sperm DNA fragmentation percent, CLU gene expression and CLU protein levels in the exposed semen samples to RF-EMF compared with non-exposed samples in OAT>AT>A>N groups, respectively (p < 0.05). Conclusion Cell phone emissions have a negative impact on exposed sperm motility index, sperm acrosin activity, sperm DNA fragmentation and seminal CLU gene expression, especially in OAT cases.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
129
136
https://www.ijfs.ir/article_45294_15db75f09df94fe2b11ed5be3d62815e.pdf
dx.doi.org/10.22074/ijfs.2015.4217
Microlithiasis of Seminal Vesicles and Severe Oligoasthenospermia in Pulmonary Alveolar Microlithiasis (PAM): Report of An Unusual Sporadic Case
Giuseppe
Castellana
District Health Center, ASL BA, via de Amicis, Conversano (70014), Bari, Italy
author
Domenico
Carone
Center of Human Reproduction and Andrology (CREA), Via Scoglio del Tonno 79/81,
Taranto (74120), Italy
author
Marco
Castellana
University of Bari, Piazza Giulio Cesare 11, Bari (70124), Italy
author
text
article
2015
eng
Pulmonary alveolar microlithiasis (PAM) is classified as an elective dysmetabolic thesaurotic pneumoalveolitis and characterized by the presence within the alveoli of the lungs of myriad of tiny calculi. The classic presentation of the chest radiography is unmistakable with multiple small "sand-like" opacities diffusely involving both lung fields. We present a case of male infertility for hypoposia and severe oligoasthenospermia in a young patient with recurrent haematuria and small calcifications in the seminal vesicles similar to pulmonary microliths. PAM was diagnosed on routine chest radiography, com- puter tomography (CT), transbronchial biopsy and bronchoalveolar lavage (BAL).
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
9
v.
1
no.
2015
137
140
https://www.ijfs.ir/article_45295_95835835b074d517ef34a134d093ed81.pdf
dx.doi.org/10.22074/ijfs.2015.4218