Association between Environmental Dioxin-Related Toxicants Exposure and Adverse Pregnancy Outcome: Systematic Review and Meta-Analysis
Xinjuan
Pan
College of Public Health, Zhengzhou University, Zhengzhou, China;Medical College, Henan University of Science and Technology, Luoyang, China
author
Xiaozhuan
Liu
College of Public Health, Zhengzhou University, Zhengzhou, China;Medical College, Henan University of Science and Technology, Luoyang, China
author
Xing
Li
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Nannan
Niu
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Xinjuan
Yin
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Ning
Li
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Zengli
Yu
College of Public Health, Zhengzhou University, Zhengzhou, China
author
text
article
2015
eng
Dioxin-related compounds are associated with teratogenic and mutagenic risks in laboratory animals, and result in adverse pregnancy outcomes. However, there were inconsistent results in epidemiology studies. In view of this difference, we conducted a systematic review and meta-analysis to examine this association and to assess the heterogeneity among studies. Comprehensive literature searches were performed to search for relevant articles published in English up to 15 May 2012. In total, we identified 15 studies which included 9 cohort and 6 case control studies. The Cochrane Q test and index of heterogeneity (I2) were used to evaluate heterogeneity. In either cohort studies (I2=0.89, p < 0.0001) or case control studies (I2=0.69, p=0.02), significant heterogeneity of risk estimates were observed. Subgroup analyses found no significant increased risk of adverse pregnancy outcome with air dioxin-related compounds exposure (RR=0.99, 95% CI:0.85–1.16), no significant increased risk of spontaneous abortion (SAB) with exposure to food dioxin-related compounds (RR=1.05, 95% CI:0.80–1.37), higher significant risks of low birth weight (LBW) with exposure to food dioxin-related compounds (RR=1.55, 95% CI:1.24–1.94), and higher significant risks of birth defects with maternal solid contaminants dioxin exposure (OR=1.24, 95% CI:1.19–1.29). In conclusion, more evidences are needed to confirm the association between environmental dioxin-related compounds exposure and pregnancy outcome.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
351
366
https://www.ijfs.ir/article_45263_a3747e9f9f8f2a86d2e79a0a96a8bc2a.pdf
dx.doi.org/10.22074/ijfs.2015.4174
Pregnancy Complications and Neonatal Outcomes in Multiple Pregnancies: A Comparison between Assisted Reproductive Techniques and Spontaneous Conception
Mahbod
Kaveh
Women Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Mahsa
Ghajarzadeh
Tehran University of Medical Sciences, Tehran, Iran
author
Fatemeh
Davari Tanha
Department of Obstetrics and Genecology, Women Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Fatemeh
Nayeri
4Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
author
Zahra
Keramati
Tehran University of Medical Sciences, Tehran, Iran
author
Mamak
Shariat
5Maternal, Fetal and Neonatal Research Center-Breastfeeding Research Center, Tehran University of Medical Sciences,
Tehran, Iran
author
Azadeh
Ghaheri
6Department of Epidemiology and Reproductive Health at Reproductive Epidemiology Research Center, Royan Institute
for Reproductive Biomedicine, ACECR, Tehran, Iran
author
text
article
2015
eng
Background This study compared neonatal outcome and maternal complications in multiple pregnancies after assisted reproductive technologies (ART) to spontaneous pregnancies. Materials and Methods In this cross-sectional study, we reviewed medical records of 190 multiple pregnancies and births conceived by ART or spontaneous conceptions between 2004 and 2009 in Women Hospital. Obstetric history and outcomes were recorded and compared between these two groups. SPSS version 13 was used for data analysis. The results were analyzed using student’s t test, chi square and logistic regression (p < 0.05). Results There were 106 deliveries from spontaneous conceptions and 84 that resulted from ART. Parity history and mode of delivery significantly differed between the two groups (p < 0.001). The ART group had significantly higher preterm labor and premature rupture of membranes (PROM) whereas pregnanc-induced hypertension (PIH) was higher in the spontaneous group (p=0.01). Newborn intensive care unit (NICU) admission, duration of hospitalization, still birth and low gestational age were significantly higher in the ART group while neonatal jaundice was higher in the spontaneous group. Logistic regression analysis by considering neonatal complications as the dependent variable showed that respiratory distress syndrome (RDS), NICU admission and Apgar score were independent predictors for neonatal complications. Conclusion Obstetric and neonatal outcomes must be considered in multiple pregnancies conceived by ART.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
367
372
https://www.ijfs.ir/article_45264_83b1dc47b15264fc8923e6296cdd86cc.pdf
dx.doi.org/10.22074/ijfs.2015.4175
The Higher Response of Vascular Endothelial Growth Factor and Angiotensin-II to Human Chorionic Gonadotropin in Women with Polycystic Ovary Syndrome
Junwei
Qu
Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital and Institute, Nanjing 210009,
China
author
Yena
Che
Translational Medicine Center and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing
University, Nanjing 210093, China
author
Pei
Xu
Translational Medicine Center and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing
University, Nanjing 210093, China
author
Yanjie
Xia
Translational Medicine Center and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing
University, Nanjing 210093, China
author
Xiaoke
Wu
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Heilongjiang University of Chinese
Medicine, Harbin 150040, China
author
Yong
Wang
Translational Medicine Center and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing
University, Nanjing 210093, China
author
text
article
2015
eng
Background This research investigated the response of vascular active factors, vascular endothelial growth factor (VEGF) and angiotensin-II (AT-II) to ovarian stimulation during 24 hours in patients with polycystic ovary syndrome (PCOS). Materials and Methods In this clinical trial study, 52 patients with PCOS and 8 control cases were stimulated with human chorionic gonadotropin (HCG) on the 4th to 7th day of the patients’ natural or induced menstrual cycles. We measured VEGF and AT-II by radioimmunoassay before the injection (0 hour) and 3, 8, 12, 18 and 24 hours after the stimulation. Results After ovarian stimulation, there was substantially higher level of VEGF in typical PCOS patients than the other three groups at the 3 hour time point (p < 0.05), while there were no significant differences in VEGF at all the other time points among the four groups. As for AT-II, before and at all time points after the ovarian stimulation, it seemed that the AT-II levels in patients’ sera with different phenotypes of PCOS by the Rotterdam criteria were all higher than in the control group although the differences were not statistically significant. The level of AT-II in typical PCOS patients was also significantly higher than the other three groups at the 3 hour time point (p < 0.05), while no significant differences at all the other time points among the four groups were observed. Conclusion The response to the stimulation varied among patients with different phenotypes of PCOS according to the Rotterdam criteria. Serum VEGF and AT-II were possible contributors to an increased risk of developing ovarian hyperstimulation syndrome (OHSS) in patients with typical PCOS during the early follicular phase (3 hours) after ovarian stimulation (Registration Number: NCT02265861).
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
373
378
https://www.ijfs.ir/article_45265_efe17ccbdda73241cbf5acec6346aaf7.pdf
dx.doi.org/10.22074/ijfs.2015.4176
The comparison between Intrauterine Insemination and Fallopian Tube Sperm Perfusion Using FAST®System in Patients with Unexplained Infertility
Sepideh
Peivandi
Department of Gynecology and Obstetrics, IVF Unit, Imam-Khomeini Hospital, Faculty of Medicine, Mazandaran
University of Medical Sciences, Sari, Iran
author
Aghdas
Ebadi
Department of Gynecology and Obstetrics, Imam-Khomeini Hospital, Faculty of Medicine, Mazandaran University
of Medical Sciences, Sari, Iran
author
Shila
Modanlu
Department of Gynecology and Obstetrics, Imam-Khomeini Hospital, Faculty of Medicine, Mazandaran University
of Medical Sciences, Sari, Iran
author
text
article
2015
eng
Background Controlled ovarian stimulation (COH) with intrauterine insemination (IUI) is commonly offered to infertile couples with patent fallopian tubes because it is simple, non-invasive and cost-effective technique. Another non-invasive method is fallopian tube sperm perfusion (FSP). This study was performed to compare the relative efficacy between FSP using fallopian sperm transfer (FAST) system and standard IUI in patients with unexplained infertility. Materials and Methods This prospective randomized study was conducted at the IVF Unit, Department of Gynecology and Obstetrics, Mazandaran University of Medical Sciences, Sari, Iran, from March 2011 to February 2012. A total of ninety patients with unexplained infertility underwent ovarian stimulation with clomiphene citrate and human menopausal gonadotropin (HMG). Patients were then randomly assigned into either group I (n=45) to undergo standard IUI or group II (n=45) to undergo FSP using FAST system. Results The patients’ basic characteristics, including age, primary infertility and duration of infertility, were not significantly different between two study groups. In the group I, there were 9 pregnancies (a pregnancy rate per cycle of 20%), whereas in the group II, 8 pregnancies occurred (a pregnancy rate per cycle of 17.8%, p > 0.05). Conclusion FSP using FAST system offers no advantage over the standard IUI in order to increase pregnancy rate in patients with unexplained infertility.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
379
384
https://www.ijfs.ir/article_45266_9fdf60c997201e49bf0ccb035d265bbe.pdf
dx.doi.org/10.22074/ijfs.2015.4177
Efficacy of Highly Purified Urinary FSH versus Recombinant FSH in Chinese Women over 37 Years Undergoing Assisted Reproductive Techniques
Xuemei
Liu
author
Cuifang
Hao
author
Jinfang
Wang
author
text
article
2015
eng
Background Urine derived follicle-stimulating hormone (uFSH) contains a higher proportion of acidic isoforms, whereas recombinant FSH (rFSH) contains a higher proportion of less-acidic isoforms. Less-acidic isoforms have a faster clearance, and thus a shorter half-life than the acidic FSH isoforms. The slow clearance of the acidic isoforms has a longer half-life and higher biological activity. This study was designed to determine whether uFSH or rFSH is more effective in older Chinese women undergoing assisted reproductive techniques (ART). Materials and Methods This is a prospective, randomized, controlled cohort study. A total of 508 Chinese women over 37 years were randomized into two following study groups for their in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles: i. group A (n=254) were treated with rFSH, and ii. group B (n=254) were treated with uFSH. Both groups were suppressed with a gonadotropin-releasing hormone (GnRH) analogue using a long down-regulation protocol. The main outcomes for comparison were days of stimulation, estradiol (E2) on the day of human chorionic gonadotropin (hCG) administration, number of oocytes collected, amount of FSH used, quantity of FSH/oocyte, endometrial thickness at hCG day, M П oocyte rate, 2PN zygote rate, grade І embryo rate, number of embryos cryopreserved, pregnancy rate, implantation rate, abortion rate and the rate of no transferable embryos. Results Twenty two cycles including 16 cycles with poor ovarian response and six cycles with ovarian hyperstimulation syndrome were cancelled. There were 243 cycles left in each group. The patients treated with uFSH had a significantly higher 2PN zygote rate (87.4 vs. 76.6%, p < 0.001), grade І embryo rate (49.8 vs. 40.8%, p < 0.001) and endometrial thickness on day of hCG (11.8 mm vs. 11.2 mm, respectively, p=0.006) and a lower rate of no transferable embryos (1.2 vs. 5.3%, p=0.019) than women treated with rFSH. The other measures evaluated showed no statistically significant differences between groups (p > 0.05). Conclusion This study showed that uFSH produced a significantly higher proportion of grade І embryos than rFSH in older Chinese women and there was a significantly lower chance of no transferable embryos in uFSH cycles. The clinical efficacy of the two gonadotropins was equivalent.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
385
392
https://www.ijfs.ir/article_45267_af68e6af166ec8959ada65c907925057.pdf
dx.doi.org/10.22074/ijfs.2015.4178
The Correlations of Anti-Mullerian Hormone, Follicle-Stimulating Hormone and Antral Follicle Count in Different Age Groups of Infertile Women
Ludmila
Barbakadze
Department of Reproductology, Obstetrics and Gynecology, Medical Faculty, Ivane Javakhishvili Tbilisi State
University, Tbilisi, Georgia
author
Jenara
Kristesashvili
Department of Reproductology, Obstetrics and Gynecology, Medical Faculty, Ivane Javakhishvili Tbilisi State
University, Tbilisi, Georgia
author
Natalia
Khonelidze
Clinic for IVF and Human Reproductive Health, Tbilisi, Georgia
author
Gia
Tsagareishvili
Clinic for IVF and Human Reproductive Health, Tbilisi, Georgia
author
text
article
2015
eng
Background The objective of our study was to identify the correlations between the tests currently used in ovarian reserve assessment: anti-Mullerian hormone (AMH), follicle stimulating hormone (FSH) and antral follicle count (AFC) and to distinguish the most reliable markers for ovarian reserve in order to select an adequate strategy for the initial stages of infertility treatment. Materials and Methods In this prospective study, 112 infertile women were assessed. Subjects were divided into three age groups: group I
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
393
398
https://www.ijfs.ir/article_45268_68fa1c7cb57ccd0e613740ef2750a41b.pdf
dx.doi.org/10.22074/ijfs.2015.4179
Do Endometrial Movements Affect The Achievement of Pregnancy during Intrauterine Insemination?
Ari
Kim
Department of Obstetrics and Gynecology, Institute of Wonkwang Medical Science, College of Medicine,
Wonkwang University, Iksan, Korea
author
Ji
Young Lee
Department of Obstetrics and Gynecology, College of Medicine, Konkuk University, Seoul, Korea
author
Yong
Il Ji
Department of Obstetrics and Gynecology, College of Medicine, Inje University, Busan, Korea
author
Hae
Hyeog Lee
4Department of Obstetrics and Gynecology, College of Medicine, Soon Chun Hyang University,
Seoul, Korea
author
Eun
Sil Lee
4Department of Obstetrics and Gynecology, College of Medicine, Soon Chun Hyang University,
Seoul, Korea
author
Heung
Yeol Kim
5Department of Obstetrics and Gynecology, College of Medicine, Kosin University, Busan, Korea
author
text
article
2015
eng
Background This study was aimed to assess the effect of endometrial movements on pregnancy achievement in intrauterine insemination (IUI) cycles. Materials and Methods The population of this observational study was composed of unexplained infertility couples undergoing first-time IUI with clomiphene citrate between September 2010 and October 2011. Not only endometrial movements, but also thickness, volume, pattern, and echogenic change of endometrium were analyzed prospectively in prediction of pregnancy. Results The total number of 241 cycles of IUI with 49 intrauterine pregnancies (20.3%) was analyzed. Pregnancy was not related to endometrial thickness and endometrial volume, but significantly related to endometrial movements associated with the number of contraction, strong movement, cervicofundal direction, and hyperechoic change (p < 0.05). Pregnant group showed higher cervicofundal movement rate (89.8 vs. 75.5%). Conclusion For IUI cycles stimulated by clomiphene citrate in unexplained infertility women, endometrial movements on the day of IUI could be a predictor of pregnancy.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
399
408
https://www.ijfs.ir/article_45269_0f9531a8ff1bfda9e15b2a7a79845468.pdf
dx.doi.org/10.22074/ijfs.2015.4180
The Social Consequences of Infertility among Iranian Women: A Qualitative Study
Syedeh Batool
Hasanpoor-Azghdy
Department of Reproductive Health and Midwifery, Faculty of Nursing and Midwifery, Iran University of Medical
Science, Tehran, Iran
author
Masoumeh
Simbar
The Research Center for Safe Motherhood , Department of Reproductive Health and Midwifery, Faculty of Nursing
and Midwifery, Shahid Beheshti Medical Sciences University, Tehran, Iran
author
Abouali
Vedadhir
Department of Anthropology, Faculty of Social Sciences, University of Tehran, Tehran, Iran
author
text
article
2015
eng
Background Infertility may prevent couples to achieve the desired social roles and lead to some social and psychological problems. This study aimed to explain the social consequences of infertility in Iranian women seeking treatment. Materials and Methods A qualitative content analysis was conducted based on 32 semi-structured interviews with 25 women affected by primary and secondary infertility with no surviving children. The participants were purposefully selected with maximum variability from a fertility health research center in Tehran, Iran, from January to October 2012. Data were collected using semi-structured interviews and analyzed using the conventional content analysis method. Results Our findings indicate that the consequences of infertility are divided into five main categories: 1. violence including psychological violence and domestic physical violence, 2. marital instability or uncertainty, 3. social isolation including avoiding certain people or certain social events and self-imposed isolation from family and friends, 4. social exclusion and partial deprivation including being disregarded by family members and relatives and reducing social interactions with the infertile woman and 5. social alienation. Conclusion This study reveals that Iranian women with fertility issues seeking treatment face several social problems that could have devastating effects on the quality of their lives. It is, therefore, recommended that, in Iran, infertility is only considered as a biomedical issue of a couple and pay further attention to its sociocultural dimensions and consequences.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
409
420
https://www.ijfs.ir/article_45270_aab9f34dcabd311b65a85063d609fd8d.pdf
dx.doi.org/10.22074/ijfs.2015.4181
Outcomes of Micro-Dissection TESE in Patients with Non-Mosaic Klinefelter’s Syndrome without Hormonal Treatment
Hakan
Ozveri
Department of Urology, Acibadem Kozyatagi Hospital, School of Medicine, Acibadem University,
Istanbul, Turkey
author
Furkan
Kayabasoglu
Istanbul Kosuyolu Gynecology Center, Istanbul, Turkey
author
Cem
Demirel
Department of Obstetrics and Gynecology, Istanbul Acibadem Hospital, Kadikoy,
Istanbul, Turkey
author
Ersan
Donmez
4Embryology Division, IVF Unit, Istanbul Acibadem Hospital, Kadikoy, Istanbul,
Turkey
author
text
article
2015
eng
Background Klinefelter syndrome (KS) is the most common sex chromosomal disorder in males and historically patients have been labeled as sterile. After the introduction of microdissection testicular sperm extraction (micro-TESE), successful sperm retrievals for intracytoplasmic sperm injection (ICSI) have been reported. Materials and Methods A retrospective study was undertaken on ten patients with non-mosaic KS undergoing micro-TESE for ICSI. The testicular volume and FSH and LH levels of each patient were measured. Karyotypes were confirmed by analyzing peripheral lymphocyte metaphases. Physical examination of the external genitalia was performed in all patients to rule out any co-existing anomaly. Micro-TESE was performed in order to investigate the presence of seminiferous tubules which may contain spermatozoa. When testicular spermatozoa were found in micro-TESE, ICSI was performed. Embryos were evaluated for further development. Fertilization was considered to have occurred after the visualization of the two pro-nuclei stage of the oocyte 24 hours after the intracytoplasmic injection of the motile spermatozoa. Pregnancy was confirmed by visualization of an intrauterine gestational sac under ultrasonographic examination. Results Testicular biopsy revealed motile spermatozoa in 6 of 9 patients (66.6 %). Fertilization rate per embryo-transfer was 40%. One patient was able to conceive and fathered a healthy boy weights 3410 g at the 39th week of gestation. Conclusion Retrieval of testicular spermatozoa by micro-TESE is possible for azoospermic men with KS when assisted reproductive techniques are applied. For patients with KS who want to conceive, assisted reproductive techniques (ART) should be recommended.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
421
428
https://www.ijfs.ir/article_45271_c616489a59fd68e4d12b940ed7875696.pdf
dx.doi.org/10.22074/ijfs.2015.4182
The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis
Longyu
Zhang
Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China;Anhui No.2 Provincial People’s Hospital, China
author
Ying
Liu
Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China
author
Yuping
Xu
Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China
author
Huan
Wu
Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China
author
Zhaolian
Wei
Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China
author
text
article
2015
eng
Background To investigate the expression of Beclin-1 mRNA and protein in eutopic and ectopic endometrium of women with and without endometriosis, and evaluate the association of Beclin-1 protein expression and serum CA125 levels in the endometriosis group due to CA125 being a well-known biomarker of endometriosis. Materials and Methods The expression levels (mean ± SD) of the mRNA and protein of Beclin-1 were examined in uterine endometria from 26 women without endometriosis and in eutopic and ectopic endometria from 26 endometriosis patients through experimental study, as reverse transcription PCR and Western-blotting assays. Serum CA125 levels in the endometriosis and control groups were compared and the correlation between Beclin-1 protein expression and serum CA125 was evaluated in the endometriosis group. Results Both eutopic (0.12 ± 0.04, 1.25 ± 0.42) and ectopic (0.12 ± 0.05, 1.09 ± 0.50) endometriotic tissue from 26 women with endometriosis expressed significantly lower levels of Beclin-1 mRNA and protein than endometrium from 26 normal women (0.15 ± 0.02, 1.67 ± 0.44) (p < 0.05). Serum CA125 levels were found to be significantly higher in the endometriosis group (p < 0.05). In addition, Beclin-1 protein expression of eutopic endometria in patients with endometriosis was negatively correlated with serum CA125 (r= -0.57, p < 0.01). Conclusion The present study strongly suggests that Beclin-1 may play a role in the formation and progression of endometriosis.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
429
436
https://www.ijfs.ir/article_45272_0b21547da5ff2ad8c9f2b7188ccc1231.pdf
dx.doi.org/10.22074/ijfs.2015.4183
The 763C>G Polymorphism of The Secretory PLA2IIa Gene Is Associated with Endometriosis in Iranian Women
Mehdi
Sahmani
Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin
University of Medical Sciences, Qazvin, Iran
author
Masoud
Darabi
Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical
Sciences, Tabriz, Iran
author
Maryam
Darabi
Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin
University of Medical Sciences, Qazvin, Iran
author
Talaat
Dabaghi
Kosar Hospital, Qazvin, Iran
author
Safar Ali
Alizadeh
Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin
University of Medical Sciences, Qazvin, Iran
author
Reza
Najafipour
Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin
University of Medical Sciences, Qazvin, Iran
author
text
article
2015
eng
Background Endometriosis is a chronic gynecological disease resulting from complex interactions between genetic, hormonal, environmental and oxidative stress and intrinsic inflammatory components. The aim of this study was to investigate the potential association of the 763C>G polymorphism in the secretory phospholipase A2 group IIa gene (PLA2G2A) with the risk of endometriosis in Iranian women. Materials and Methods Ninety seven patients with endometriosis along with 107 women who were negative for endometriosis after laparoscopy and laparatomy, and served as the control group, were enrolled for this cross-sectional study. Samples were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method. Results Multivariate analysis was used to examine the association between the risk of endometriosis and the 763C>G polymorphism of PLA2G2A. Genotype distributions of PLA2G2A were significantly different between patients and the controls (p < 0.001, OR=0.22, 95% CI=0.21-0.39). Correlation analysis showed that there was a significant association between the normal homozygous genotype and susceptibility to endometriosis (p < 0.001). Conclusion The present study suggests that the 763C>G polymorphism of PLA2G2A plays an important role as an independent factor in the risk of endometriosis in Iranian women.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
437
444
https://www.ijfs.ir/article_45273_7038a2b4adfb1281414b5278a851158c.pdf
dx.doi.org/10.22074/ijfs.2015.4184
Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy
Hai-Yan Zhu
Zhu
author
Xiao-Mei Tong
Tong
author
Xiao-Na Lin
Lin
author
Ling-Ying Jiang
Jiang
author
Jun-Xia Wang
Wang
author
Song-Ying
Zhang
author
text
article
2015
eng
Background S100P is a member of the S100 family of calcium-binding proteins, and it participates in pathophysiological events, such as tumor growth and invasion. Based on the striking similarities between trophoblast cells and tumor cells with regard to proliferative and invasive properties, we raised the question of whether and how S100P expresses in trophoblast cells during development. This study aimed to investigate the expression pattern of S100P in the human placenta during pregnancy development. Materials and Methods In this experimental study, we collected 16 first-trimester placental tissues, 10 second-trimester placental tissues, and 12 term placentas. The mRNA expression levels of S100P were detected by reverse-transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR, the protein expression levels were detected by western blot, and the localization of S100P was measured by immunohistochemical staining. The values obtained from PCR and western blot analysis were expressed as the mean ± SD. Levene’s test was used to test equal variances, and one-way analysis of variance (ANOVA) was used to evaluate differences between groups. Results Protein and mRNA expression of S100P could be detected in placenta during pregnancy, with minor higher levels in first-trimester (p > 0.05). Immunohistochemical staining revealed that S100P protein was strongly expressed in syncytiotrophoblasts, and moderate expression was detected in villous cytotrophoblasts and cytotrophoblast columns. The S100P protein was localized to both cytoplasm and nuclei in syncytiotrophoblasts, while it only existed in the cytoplasm of cytotrophoblasts. Conclusion S100P was strongly detected in human placenta during pregnancy. The specific expression and distribution of S100P in human placenta throughout gestation suggested that S100P function might vary with its location in the placenta.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
445
452
https://www.ijfs.ir/article_45274_3d285ad31759deda4dea85c4fbb1aa26.pdf
dx.doi.org/10.22074/ijfs.2015.4189
A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran
Maryam
Gholinezhad Chari
Fatemehzahra Infertility and Reproductive Health Research Center, Babol University of Medical Sciences,
Babol, Iran;Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran
author
Abasalt
Hosseinzadeh Colagar
Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
author
Ali
Bidmeshkipour
Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran
author
text
article
2015
eng
Background To investigate the level of correlation between large-scale deletions of the mitochondrial DNA (mtDNA) with defective sperm function. Materials and Methods In this analytic study, a total of 25 semen samples of the nor- mozoospermic infertile men from North of Iran were collected from the IVF center in an infertility clinic. The swim-up procedure was performed for the separation of spermatozoa into two groups; (normal motility group and abnormal motility group) by 2.0 ml of Ham’s F-10 medium and 1.0 ml of semen. After total DNA extraction, a long-range polymerase chain reaction (PCR) technique was used to determine the mtDNA deletions in human spermatozoa. Results The products of PCR analysis showed a common 4977 bp deletion and a novel 4866 bp deletion (flanked by a seven-nucleotide direct repeat of 5΄-ACCCCCT-3΄ within the deleted area) from the mtDNA of spermatozoa in both groups. However, the frequency of mtDNA deletions in abnormal motility group was significantly higher than the normal motility group (56, and 24% for 4866 bp-deleted mtDNA and, 52, and 28% for 4977 bp-deleted mtDNA, respectively). Conclusion It is suggested that large-scale deletions of the mtDNA is associated with poor sperm motility and may be a causative factor in the decline of fertility in men.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
453
463
https://www.ijfs.ir/article_45275_922785263a5e4c37d89f978d5818bd06.pdf
dx.doi.org/10.22074/ijfs.2015.4185
Association between Maternal MTHFR Polymorphisms and Nonsyndromic Cleft Lip with or without Cleft Palate in Offspring, A Meta-Analysis Based on 15 Case-Control Studies
Xinjuan
Pan
College of Public Health, Zhengzhou University, Zhengzhou, China;Medical College, Henan University of Science and Technology, Luoyang, China
author
Ping
Wang
Medical College, Henan University of Science and Technology, Luoyang, China
author
Xinjuan
Yin
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Xiaozhuan
Liu
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Di
Li
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Xing
Li
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Yongchao
Wang
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Hongle
Li
College of Public Health, Zhengzhou University, Zhengzhou, China
author
Zengli
Yu
College of Public Health, Zhengzhou University, Zhengzhou, China
author
text
article
2015
eng
Background The methylenetetrahydrofolate reductase (MTHFR) is thought to be involved in the development of nonsyndromic cleft lip with or without cleft palate (NSCL/P). However, conflicting results have been obtained when evaluating the association between maternal MTHFR C677T and A1298C polymorphisms and the risk of NSCL/P. In light of this gap, a meta-analysis of all eligible case-control studies was conducted in the present study. Materials and Methods A total of 15 case-control studies were ultimately identified after a comprehensive literature search and Hardy-Weinberg equilibrium (HWE) examination. Cochrane’s Q test and index of heterogeneity (I2) indicated no obvious heterogeneity among studies. Results Fixed or random-effects models were used to calculate the pooled odds ratios (ORs). The results showed that the TT genotype in mothers increased the likelihood of having NSCL/P offspring 1.25 times (95% CI: 1.047-1.494) more than the CC homozygotes. Meanwhile, maternal TT genotype increased the risk of producing NSCL/P offspring in recessive model (OR=1.325, 95% CI: 1.124-1.562). However, the CT heterozygote and the CT+TT dominant models had no association with NSCL/P offspring compared with the CC wild-type homozygote model. Subgroup analyses based on ethnicity indicated that maternal TT genotype increased the likelihood of having NSCL/P offspring in Whites (OR=1.308, 95% CI: 1.059-1.617) and Asians (OR=1.726, 95% CI: 1.090-2.733) in recessive model. Also, subgroup analyses based on source of control showed that mothers with the 677TT genotype had a significantly increased susceptibility of having NSCL/P children in hospital based population (HB) when compared with CC homozygotes (OR=1.248, 95% CI: 1.024-1.520) and un- der the recessive model (OR=1.324, 95% CI: 1.104-1.588). Furthermore, maternal A1298C polymorphism had no significant association with producing NSCL/P offspring (dominant model OR=0.952, 95% CI: 0.816-1.111, recessive model OR=0.766, 95% CI: 0.567-1.036). Conclusion MTHFR C677T polymorphism is associated with the risk of generating NSCL/P offspring, and being a 677TT homozygote is a risk factor. MTHFR A1298C polymorphism was not associated with generating NSCL/P offspring. However, further work should be performed to confirm these findings.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
463
480
https://www.ijfs.ir/article_45276_36f505cb831fa4a64a23ceb2f1eeba3c.pdf
dx.doi.org/10.22074/ijfs.2015.4186
Tuberculosis Endometritis Presenting as A Leiomyoma
Mahboobeh
Shirazi
Maternal, Fetal and Neonatal Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences,
Tehran, Iran
author
Fatemeh
Shahbazi
Department of Biology, Payame Noor University, Iran
author
Leila
Pirzadeh
Maternal, Fetal and Neonatal Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences,
Tehran, Iran
author
Seyed Rahim
Mohammadi
Maternal, Fetal and Neonatal Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences,
Tehran, Iran
author
Parisa
Ghaffari
Maternal, Fetal and Neonatal Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences,
Tehran, Iran
author
Tahereh
Eftekhar
Maternal, Fetal and Neonatal Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences,
Tehran, Iran;Obstetrics Gynecology, Gynecology Ward, Emam Hospital, Keshavarz blvd,
Tehran, Iran
author
text
article
2015
eng
Genitourinary tuberculosis is a common extrapulmonary manifestation of tuberculosis. Taking into consideration that genitourinary tuberculosis may be associated with a diversity of presentations, its diagnoses may be difficult. A young woman with an initial presumptive diagnosis of a uterine leiomyoma presented with abdominal pain and a pelvic mass that after further investigations, she was diagnosed with genital tuberculosis.
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
481
484
https://www.ijfs.ir/article_45277_29a8fc6fab9649ab981848d668311da7.pdf
dx.doi.org/10.22074/ijfs.2015.4187
Methodological Concerns Regarding Levels of Vascular Endothelial Growth Factor (VEGF) in Serum of Women with Endometriosis
Umberto
Leone Roberti Maggiore
Department of Obstetrics and Gynecology, San Martino Hospital and National Institute for Cancer Research, University of Genoa, Genoa, Italy
author
Simone
Ferrero
Department of Obstetrics and Gynecology, San Martino Hospital and National Institute for Cancer Research, University of Genoa, Genoa, Italy
author
text
article
2015
eng
International Journal of Fertility and Sterility
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
2008-076X
8
v.
4
no.
2015
485
486
https://www.ijfs.ir/article_45278_c4b57933f0113713ffb8776bfff3dc4e.pdf
dx.doi.org/10.22074/ijfs.2015.4188