Past Issue

Volume 7, Number 4, Jan-Mar 2014, Pages: 313-322

Protective Effect of Melatonin against Inequality-Induced Da mages on Testicular Tissue and Sper m Para meters


Shiva Nasiraei-Moghadam Nasiraei-Moghadam, Ph.D, 1, *, Kazem Parivar, Ph.D, 1, Abolhasan Ahmadiani, Ph.D, 2, Mansoureh Movahhedin, Ph.D, 3, Mohammad Reza Vaez Mahdavi, Ph.D, 4,
Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Clinical Trial Iranian Traditional Medicine Research Center and Health Equity, College of Medicine, Shahed University, Tehran, Iran
* Corresponding Address: P.O.Box: 1477893855 Department of Biology Faculty of Basic Sciences Science and Research Branch Islamic Azad University Tehran Iran Email: shiva_nasiraei@yahoo.com

Abstract

Background:

The goals of the study are evaluation the effects of food deprivation and isolation situation as a social stress on fertility; and in the following, investigation of the improving effect of melatonin as an antioxidant component.

Materials and Methods:

In this experimental study, We investigated histopathological and serological effects of melatonin and social stress (food deprivation and isolation) on different features of sperm and testicular tissue among 42 male rats in 7 groups including control, sham, melatonin received (M), food deprivation (FD), Food deprivation and melatonin treatment (FDM), Food deprivation and isolation situation (FDi), and Food deprivation and melatonin treatment and isolation situation (FDMi) groups. Epididymal sperms of all rats were also counted. Histopathological evaluation of the testes was done under a light microscopy to determine the number of spermiogenic cells. Serological evaluation of testosterone, corticosterone, and melatonin was performed, as well. For statistical analysis, oneway ANOVA and Tukey’s post hoc test were used, and the value of p≤0.05 was considered statistically significance.

Results:

The result showed that food deprivation increased the number of abnormal, immotile, and dead sperms, while decreased the number of normal sperms (p<0.05). Isolation could improve sperm motility and viability, while enhanced the number of sper- matogenic cells. Melatonin had a protective effect on sperm count, motility, and viability, while reduced sperm abnormality.

Conclusion

Our results demonstrated that melatonin treatment and isolation situation improve the parameters related to epididymal sperms and spermatogenic cells after food deprivation.