Journal Title: Volume 3,Number 3,Nov-Dec 2009, Pages: 87-156
Page From: 111 To: 118
Article title: Comparison of Different Vitrification Procedures on Developmental Competence of Mouse Germinal Vesicle Oocytes in the Presence or Absence of Cumulus Cells
Background: An evaluation of the developmental competence of vitrified mouse germinal vesicle
(GV) oocytes with various equilibration and vitrification times; in the presence or absence of
cumulus cells and by comparison between the cryotop method and straws was performed.
Materials and Methods: Mouse GV oocytes were considered in cumulus-denuded oocytes
(CDOs) and cumulus-oocyte complexes (COCs) groups. Their survival and developmental rates
were studied in the following experiments: (I) exposure to different equilibration times (0, 3 and
5 minutes) and vitrification (1, 3 and 5 minutes) without plunging in LN2 as toxicity tests, (II)
oocytes were vitrified using straws followed by exposure to equilibration solution for 0, 3 and 5
minutes and vitrification solution for 1 and 3 minutes, and (III) oocytes were vitrified by cryotop
following exposure to equilibration for 5 minutes and vitrification for 1 minute, respectively.
Results: Maturation and developmental rates of the COCs were higher than CDOs in the nonvitrified
group (p<0.05). The survival and maturation rates were low in all oocytes exposed to
vitrification solution for 5 minutes (p <0.05). In vitrified CDOs and COCs using straws, the survival
rates ranged from 56.9% to 85.4% and 44.0% to 84.5%, and the maturation rates from 35.3% to
56.8% and 25.8% to 56.2%, respectively; which were lower than non-vitrified samples (p <0.05).
Cryotop vitrified oocytes showed higher survival, maturation and fertilization rates when compared
to straw in both CDOs and COCs (p <0.05).
Conclusion: The presence of cumulus cells improves developmental competence of GV oocytes
in control groups but it did not affect the vitrified group. Vitrification of mouse GV oocytes using
cryotop was more effective than straws, however both vitrification techniques did not improve the
Oocytes, Cryopreservation, Cumulus Cells, In vitro
Received: 14 Jun 2009 Accepted: 19 Sep 2009
* Corresponding Address: P.O.Box: 14115-111 Anatomy Department
Faculty of Medical Sciences Tarbiat Modares University Tehran Iran
|Saeed Zavareh ||First Author|
|Mojdeh Salehnia ||Other Authorfirstname.lastname@example.org|
|Adel Saberivand ||Other Author|